Identification of patients arm microorganisms to improve existing disinfection practices in phlebotomy / Nur Najihah Mohd Raslam

Phlebotomist plays a crucial role in the pre-analytical phase as they need to perform venipuncture for collecting blood specimen for most of the blood tests. Before a venipuncture process, the patient’s antecubital areas need to disinfect thoroughly to minimize contamination, particularly from human...

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Main Author:	Mohd Raslam, Nur Najihah
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Cultures and culture media Culture technique. Bacteria Examination of the blood
Online Access:	https://ir.uitm.edu.my/id/eprint/50744/1/50744.pdf
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id	my-uitm-ir.50744
record_format	uketd_dc
spelling	my-uitm-ir.507442021-09-28T03:14:44Z Identification of patients arm microorganisms to improve existing disinfection practices in phlebotomy / Nur Najihah Mohd Raslam 2019-07 Mohd Raslam, Nur Najihah Cultures and culture media. Culture technique. Bacteria Public health. Hygiene. Preventive Medicine Examination of the blood Phlebotomist plays a crucial role in the pre-analytical phase as they need to perform venipuncture for collecting blood specimen for most of the blood tests. Before a venipuncture process, the patient’s antecubital areas need to disinfect thoroughly to minimize contamination, particularly from human skin normal flora. The hand microbiome compositions are affected by many sources of variability, both intrinsic and extrinsic factors. The study of hand microbiome can help in understanding hostorganism relationships thus helping in the use of potent antimicrobial agents on the patient’s arm to maximize disinfection prior to blood donation or blood culture collection, as these two are critical for microbiological contamination. The objectives of this study are to identify the commonest isolated microorganisms at the antecubital fossa of study group by gram stain and biochemical test, to determine the contribution of demographic and hygienic practice to the prevalence of the microorganisms, and to identify whether the routine aseptic technique is adequate to minimize the risk of infection. In this study, the most isolated organisms were Staphylococcus epidermidis (46%) (12/26), followed by another coagulase-negative Staphylococcus (CoNS) (35%) (9/26), and Staphylococcus capitis (19%) (5/26). There was no significant association between study group characteristics (gender, dominant hand, daily bath frequency, lotion or perfume usage, and preference of clothes) with the prevalence of the microorganism present (p>0.05). The mean log₁₀ of colony-forming units (CFUs) pre- and postdisinfection with 70% isopropyl alcohol shows a significant reduction (Paired t-test, p=0.002) indicating appropriate measures of current practice in eliminating the risk of microbiological contamination. In conclusion, the presence of normal floras that can become pathogens in certain individual ascertained that disinfecting the skin before venipuncture is a crucial step to avoid false-positive blood culture and to decrease contamination of blood and blood products by both resident and transient skin floras. By studying the microbiome on the antecubital fossa, an approach and finding new antiseptic agents to improve antimicrobial agent efficacy in eliminating resident and transient floras prior to blood donation or blood culture collection can be improved. 2019-07 Thesis https://ir.uitm.edu.my/id/eprint/50744/ https://ir.uitm.edu.my/id/eprint/50744/1/50744.pdf text en public degree Universiti Teknologi MARA ( Kampus Puncak Alam ) Faculty of Health Sciences Mastuki, Mohd Fahmi
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Mastuki, Mohd Fahmi
topic	Cultures and culture media Culture technique. Bacteria Cultures and culture media Culture technique. Examination of the blood
spellingShingle	Cultures and culture media Culture technique. Bacteria Cultures and culture media Culture technique. Examination of the blood Mohd Raslam, Nur Najihah Identification of patients arm microorganisms to improve existing disinfection practices in phlebotomy / Nur Najihah Mohd Raslam
description	Phlebotomist plays a crucial role in the pre-analytical phase as they need to perform venipuncture for collecting blood specimen for most of the blood tests. Before a venipuncture process, the patient’s antecubital areas need to disinfect thoroughly to minimize contamination, particularly from human skin normal flora. The hand microbiome compositions are affected by many sources of variability, both intrinsic and extrinsic factors. The study of hand microbiome can help in understanding hostorganism relationships thus helping in the use of potent antimicrobial agents on the patient’s arm to maximize disinfection prior to blood donation or blood culture collection, as these two are critical for microbiological contamination. The objectives of this study are to identify the commonest isolated microorganisms at the antecubital fossa of study group by gram stain and biochemical test, to determine the contribution of demographic and hygienic practice to the prevalence of the microorganisms, and to identify whether the routine aseptic technique is adequate to minimize the risk of infection. In this study, the most isolated organisms were Staphylococcus epidermidis (46%) (12/26), followed by another coagulase-negative Staphylococcus (CoNS) (35%) (9/26), and Staphylococcus capitis (19%) (5/26). There was no significant association between study group characteristics (gender, dominant hand, daily bath frequency, lotion or perfume usage, and preference of clothes) with the prevalence of the microorganism present (p>0.05). The mean log₁₀ of colony-forming units (CFUs) pre- and postdisinfection with 70% isopropyl alcohol shows a significant reduction (Paired t-test, p=0.002) indicating appropriate measures of current practice in eliminating the risk of microbiological contamination. In conclusion, the presence of normal floras that can become pathogens in certain individual ascertained that disinfecting the skin before venipuncture is a crucial step to avoid false-positive blood culture and to decrease contamination of blood and blood products by both resident and transient skin floras. By studying the microbiome on the antecubital fossa, an approach and finding new antiseptic agents to improve antimicrobial agent efficacy in eliminating resident and transient floras prior to blood donation or blood culture collection can be improved.
format	Thesis
qualification_level	Bachelor degree
author	Mohd Raslam, Nur Najihah
author_facet	Mohd Raslam, Nur Najihah
author_sort	Mohd Raslam, Nur Najihah
title	Identification of patients arm microorganisms to improve existing disinfection practices in phlebotomy / Nur Najihah Mohd Raslam
title_short	Identification of patients arm microorganisms to improve existing disinfection practices in phlebotomy / Nur Najihah Mohd Raslam
title_full	Identification of patients arm microorganisms to improve existing disinfection practices in phlebotomy / Nur Najihah Mohd Raslam
title_fullStr	Identification of patients arm microorganisms to improve existing disinfection practices in phlebotomy / Nur Najihah Mohd Raslam
title_full_unstemmed	Identification of patients arm microorganisms to improve existing disinfection practices in phlebotomy / Nur Najihah Mohd Raslam
title_sort	identification of patients arm microorganisms to improve existing disinfection practices in phlebotomy / nur najihah mohd raslam
granting_institution	Universiti Teknologi MARA ( Kampus Puncak Alam )
granting_department	Faculty of Health Sciences
publishDate	2019
url	https://ir.uitm.edu.my/id/eprint/50744/1/50744.pdf
_version_	1783734839520788480

Identification of patients arm microorganisms to improve existing disinfection practices in phlebotomy / Nur Najihah Mohd Raslam

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