Antimicrobial activity of allium sativum extract against antecubital fossa bacteria / Nurul Huda Nabilah Halim

Certainly, different techniques of reducing a load of bacterial contamination at antecubital fossa have been attempted and always being improvised especially for phlebotomy procedure. People have tried to explore the healing power of different plant extract and treat disease. Several studies have re...

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Main Author:	Halim, Nurul Huda Nabilah
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Botanical chemistry Phytochemicals Bacteria Vegetables
Online Access:	https://ir.uitm.edu.my/id/eprint/50755/1/50755.pdf
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id	my-uitm-ir.50755
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spelling	my-uitm-ir.507552021-09-28T03:10:39Z Antimicrobial activity of allium sativum extract against antecubital fossa bacteria / Nurul Huda Nabilah Halim 2019-07 Halim, Nurul Huda Nabilah Botanical chemistry. Phytochemicals Bacteria Vegetables Certainly, different techniques of reducing a load of bacterial contamination at antecubital fossa have been attempted and always being improvised especially for phlebotomy procedure. People have tried to explore the healing power of different plant extract and treat disease. Several studies have reported that there are some contradict about the effectiveness of commercial antiseptic in reduce bacterial contamination. Some studies on hand disinfection with alcohol-based hand rubs showed that during the hand rubbing procedure, users are exposed to these alcohols not only through dermal contact but also via inhalation. This is due to physical and chemical properties volatizing from alcoholic solutions or gels into the air. Therefore, identification of antimicrobial activity in Allium sativum (garlic) against bacterial skin especially on antecubital fossa was studied for the potential uses as alternatives antiseptic. Three types of extracts were used in this study which were aqueous garlic extract (AGE), ethanolic garlic extract (EGE) and ethanolic garlic powder extract (EGPE). Their effects were measured against normal flora and pathogenic bacteria namely Staphylococcus aureus (ATCC 25923), Staphylococcus epidermidis (ATCC 1228), Streptococcus pyogenes (ATCC 25922) and Pseudomonas aeruginosa (ATCC 10145) using well diffusion technique. EGPE was unable to produce any inhibition zone against all tested bacteria. Out of four bacteria tested, three bacteria were found to be sensitive towards AGE and EGE including S. aureus, S.epidermidis and S. pyogenes. On the other hand, P. aeruginosa only sensitive to EGE and exhibited high resistance to AGE. AGE and EGE showed promising inhibitory activity even in low concentration against S. pyogenes with minimal inhibitory concentration (MIC) value of 125 mg/ml and 15.63 mg/mL, respectively. Both extracts showed significant antimicrobial effect against S. aureus at 62.5 mg/mL while S. epidermidis at 7.81 mg/mL. EGE also showed significant antimicrobial effect against P. aeruginosa until with MIC value of 62.5 mg/mL. In conclusion, Allium sativum extracts showed substantial antimicrobial activity against the majority of the tested bacteria (p < 0.05) and it can be a good source of antimicrobial agents thus it can become an alternative to the commercial alcohol-based antiseptic. 2019-07 Thesis https://ir.uitm.edu.my/id/eprint/50755/ https://ir.uitm.edu.my/id/eprint/50755/1/50755.pdf text en public degree Universiti Teknologi MARA ( Kampus Puncak Alam ) Faculty of Health Sciences Mastuki, Mohd Fahmi
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Mastuki, Mohd Fahmi
topic	Botanical chemistry Phytochemicals Bacteria Vegetables
spellingShingle	Botanical chemistry Phytochemicals Bacteria Vegetables Halim, Nurul Huda Nabilah Antimicrobial activity of allium sativum extract against antecubital fossa bacteria / Nurul Huda Nabilah Halim
description	Certainly, different techniques of reducing a load of bacterial contamination at antecubital fossa have been attempted and always being improvised especially for phlebotomy procedure. People have tried to explore the healing power of different plant extract and treat disease. Several studies have reported that there are some contradict about the effectiveness of commercial antiseptic in reduce bacterial contamination. Some studies on hand disinfection with alcohol-based hand rubs showed that during the hand rubbing procedure, users are exposed to these alcohols not only through dermal contact but also via inhalation. This is due to physical and chemical properties volatizing from alcoholic solutions or gels into the air. Therefore, identification of antimicrobial activity in Allium sativum (garlic) against bacterial skin especially on antecubital fossa was studied for the potential uses as alternatives antiseptic. Three types of extracts were used in this study which were aqueous garlic extract (AGE), ethanolic garlic extract (EGE) and ethanolic garlic powder extract (EGPE). Their effects were measured against normal flora and pathogenic bacteria namely Staphylococcus aureus (ATCC 25923), Staphylococcus epidermidis (ATCC 1228), Streptococcus pyogenes (ATCC 25922) and Pseudomonas aeruginosa (ATCC 10145) using well diffusion technique. EGPE was unable to produce any inhibition zone against all tested bacteria. Out of four bacteria tested, three bacteria were found to be sensitive towards AGE and EGE including S. aureus, S.epidermidis and S. pyogenes. On the other hand, P. aeruginosa only sensitive to EGE and exhibited high resistance to AGE. AGE and EGE showed promising inhibitory activity even in low concentration against S. pyogenes with minimal inhibitory concentration (MIC) value of 125 mg/ml and 15.63 mg/mL, respectively. Both extracts showed significant antimicrobial effect against S. aureus at 62.5 mg/mL while S. epidermidis at 7.81 mg/mL. EGE also showed significant antimicrobial effect against P. aeruginosa until with MIC value of 62.5 mg/mL. In conclusion, Allium sativum extracts showed substantial antimicrobial activity against the majority of the tested bacteria (p < 0.05) and it can be a good source of antimicrobial agents thus it can become an alternative to the commercial alcohol-based antiseptic.
format	Thesis
qualification_level	Bachelor degree
author	Halim, Nurul Huda Nabilah
author_facet	Halim, Nurul Huda Nabilah
author_sort	Halim, Nurul Huda Nabilah
title	Antimicrobial activity of allium sativum extract against antecubital fossa bacteria / Nurul Huda Nabilah Halim
title_short	Antimicrobial activity of allium sativum extract against antecubital fossa bacteria / Nurul Huda Nabilah Halim
title_full	Antimicrobial activity of allium sativum extract against antecubital fossa bacteria / Nurul Huda Nabilah Halim
title_fullStr	Antimicrobial activity of allium sativum extract against antecubital fossa bacteria / Nurul Huda Nabilah Halim
title_full_unstemmed	Antimicrobial activity of allium sativum extract against antecubital fossa bacteria / Nurul Huda Nabilah Halim
title_sort	antimicrobial activity of allium sativum extract against antecubital fossa bacteria / nurul huda nabilah halim
granting_institution	Universiti Teknologi MARA ( Kampus Puncak Alam )
granting_department	Faculty of Health Sciences
publishDate	2019
url	https://ir.uitm.edu.my/id/eprint/50755/1/50755.pdf
_version_	1783734839802855424

Antimicrobial activity of allium sativum extract against antecubital fossa bacteria / Nurul Huda Nabilah Halim

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