Characterization and corfirmation of lactobacillus spp. isolated from dairy products: biochemical, pribiotic potential and molecular study / Herys Elvino Jaikol @ Yaikol

For half a century, the global community had been highly dependent towards antibiotic as a treatment against bacterial infection, give arise to new form of mutate organism capable of resisting against several type of antibiotics. Ceaseless demand for an alternative of antibiotic is highly sought to...

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Main Author:	Jaikol @ Yaikol, Herys Elvino
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Physical and theoretical chemistry Bacteria
Online Access:	https://ir.uitm.edu.my/id/eprint/50950/1/50950.pdf
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id	my-uitm-ir.50950
record_format	uketd_dc
spelling	my-uitm-ir.509502021-09-28T04:03:55Z Characterization and corfirmation of lactobacillus spp. isolated from dairy products: biochemical, pribiotic potential and molecular study / Herys Elvino Jaikol @ Yaikol 2019-07 Jaikol @ Yaikol, Herys Elvino Physical and theoretical chemistry Bacteria Personal health and hygiene. Including clothing, bathing, exercise, travel, nutrition, sleep, sex hygiene For half a century, the global community had been highly dependent towards antibiotic as a treatment against bacterial infection, give arise to new form of mutate organism capable of resisting against several type of antibiotics. Ceaseless demand for an alternative of antibiotic is highly sought to overcome such issue. Lactobacillus spp. was a type of Lactic Acid Bacteria (LAB) which are well-known for their probiotic potential towards human health. However, in Malaysia, information regarding this organism are still deficient due to limited amount of research conducted. In an attempt to gain information about this matter, the characterization and confirmation study of Lactobacillus spp. isolated from dairy product and their probiotic potential by using molecular and biochemical method was conducted. The aims of this research was actually to identify and characterize the biochemistry, tolerance to bile and antibiotic susceptibility profiles of putative Lactobacillus spp. isolated from dairy products and further confirm whether the isolates of Lactobacillus spp. belonged from amplified 16S rRNA gene. Eleven (11) isolates was isolated from two sources of sample; 8 isolates from goat’s milk, 3 isolates from Tairu’s Buffalo Milk. Each isolates was characterized by using biochemical test, while Bile Salt Tolerant and Antibiotic Susceptibility Test (AST) method was used for determination of potential probiotic property. A further confirmation was done by using Polymerase Chain Reaction (PCR) assay of 16S rRNA. Biochemical test showed 7 isolates have similar characteristics toward the Lactobacillus spp., which are catalase and oxidase negative, Gram-positive rod organism and shown the same pattern in susceptibility of several antibiotic except for Vancomycin also have tolerance against 0.3% bile salt activity. These 7 isolates were further confirmed belonged to Lactobacillus genus as exhibited by 566 base pair (bp) amplicon. Based on the findings, it can be suggested that the putative Lactobacillus isolates have shown a promising potential as a probiotic organism. 2019-07 Thesis https://ir.uitm.edu.my/id/eprint/50950/ https://ir.uitm.edu.my/id/eprint/50950/1/50950.pdf text en public degree Universiti Teknologi MARA ( Kampus Puncak Alam ) Faculty of Health Sciences Mustakim, Maimunah
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Mustakim, Maimunah
topic	Physical and theoretical chemistry Bacteria Physical and theoretical chemistry
spellingShingle	Physical and theoretical chemistry Bacteria Physical and theoretical chemistry Jaikol @ Yaikol, Herys Elvino Characterization and corfirmation of lactobacillus spp. isolated from dairy products: biochemical, pribiotic potential and molecular study / Herys Elvino Jaikol @ Yaikol
description	For half a century, the global community had been highly dependent towards antibiotic as a treatment against bacterial infection, give arise to new form of mutate organism capable of resisting against several type of antibiotics. Ceaseless demand for an alternative of antibiotic is highly sought to overcome such issue. Lactobacillus spp. was a type of Lactic Acid Bacteria (LAB) which are well-known for their probiotic potential towards human health. However, in Malaysia, information regarding this organism are still deficient due to limited amount of research conducted. In an attempt to gain information about this matter, the characterization and confirmation study of Lactobacillus spp. isolated from dairy product and their probiotic potential by using molecular and biochemical method was conducted. The aims of this research was actually to identify and characterize the biochemistry, tolerance to bile and antibiotic susceptibility profiles of putative Lactobacillus spp. isolated from dairy products and further confirm whether the isolates of Lactobacillus spp. belonged from amplified 16S rRNA gene. Eleven (11) isolates was isolated from two sources of sample; 8 isolates from goat’s milk, 3 isolates from Tairu’s Buffalo Milk. Each isolates was characterized by using biochemical test, while Bile Salt Tolerant and Antibiotic Susceptibility Test (AST) method was used for determination of potential probiotic property. A further confirmation was done by using Polymerase Chain Reaction (PCR) assay of 16S rRNA. Biochemical test showed 7 isolates have similar characteristics toward the Lactobacillus spp., which are catalase and oxidase negative, Gram-positive rod organism and shown the same pattern in susceptibility of several antibiotic except for Vancomycin also have tolerance against 0.3% bile salt activity. These 7 isolates were further confirmed belonged to Lactobacillus genus as exhibited by 566 base pair (bp) amplicon. Based on the findings, it can be suggested that the putative Lactobacillus isolates have shown a promising potential as a probiotic organism.
format	Thesis
qualification_level	Bachelor degree
author	Jaikol @ Yaikol, Herys Elvino
author_facet	Jaikol @ Yaikol, Herys Elvino
author_sort	Jaikol @ Yaikol, Herys Elvino
title	Characterization and corfirmation of lactobacillus spp. isolated from dairy products: biochemical, pribiotic potential and molecular study / Herys Elvino Jaikol @ Yaikol
title_short	Characterization and corfirmation of lactobacillus spp. isolated from dairy products: biochemical, pribiotic potential and molecular study / Herys Elvino Jaikol @ Yaikol
title_full	Characterization and corfirmation of lactobacillus spp. isolated from dairy products: biochemical, pribiotic potential and molecular study / Herys Elvino Jaikol @ Yaikol
title_fullStr	Characterization and corfirmation of lactobacillus spp. isolated from dairy products: biochemical, pribiotic potential and molecular study / Herys Elvino Jaikol @ Yaikol
title_full_unstemmed	Characterization and corfirmation of lactobacillus spp. isolated from dairy products: biochemical, pribiotic potential and molecular study / Herys Elvino Jaikol @ Yaikol
title_sort	characterization and corfirmation of lactobacillus spp. isolated from dairy products: biochemical, pribiotic potential and molecular study / herys elvino jaikol @ yaikol
granting_institution	Universiti Teknologi MARA ( Kampus Puncak Alam )
granting_department	Faculty of Health Sciences
publishDate	2019
url	https://ir.uitm.edu.my/id/eprint/50950/1/50950.pdf
_version_	1783734841326436352

Characterization and corfirmation of lactobacillus spp. isolated from dairy products: biochemical, pribiotic potential and molecular study / Herys Elvino Jaikol @ Yaikol

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