Larvicidal activities of citrus hystrix (kaffir lime) leaf extract against aedes albopictus / Nik Mohamed Adzfar Mohamed Resli

Aedes mosquitoes are widespread, and mosquito control efforts are essential to prevent outbreaks of many arboviruses such as dengue, zika and chikungunya. At present, chemical insecticides are often utilised for this purpose, which can cause long term environmental effects and the evolution of resis...

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Main Author:	Mohamed Resli, Nik Mohamed Adzfar
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Bioactive compounds Diptera (Flies) Fruit and fruit culture
Online Access:	https://ir.uitm.edu.my/id/eprint/51081/1/51081.pdf
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id	my-uitm-ir.51081
record_format	uketd_dc
spelling	my-uitm-ir.510812021-09-28T08:47:04Z Larvicidal activities of citrus hystrix (kaffir lime) leaf extract against aedes albopictus / Nik Mohamed Adzfar Mohamed Resli 2019-07 Mohamed Resli, Nik Mohamed Adzfar Bioactive compounds Diptera (Flies) Fruit and fruit culture Aedes mosquitoes are widespread, and mosquito control efforts are essential to prevent outbreaks of many arboviruses such as dengue, zika and chikungunya. At present, chemical insecticides are often utilised for this purpose, which can cause long term environmental effects and the evolution of resistance if not controlled. This study was conducted to determine the efficacy of Citrus hystrix (kaffir lime) leaf extract act as a natural alternative larvicide to substitute the use of chemicals. The method of extraction used was maceration digestion. It is a modified form of maceration which involves drying, crushing, soaking with gentle heat and removal of solvent to obtain a pure crude extract. A total 25 larvae were used to test against five (5) different concentrations (50, 40, 30, 20 and 10 ppm) of Citrus hystrix for 24 - 48 hrs and evaluated in terms of mortality rates. Experimental tests were conducted in triplicate and analysed using probit analysis and analysis of variance (ANOVA). Three types of control were used in tandem namely positive (11 mg abate), negative (distilled water) and quality control (10% (v/v) solvent) to ensure the reliability of the test results. Findings revealed 100% effectiveness at 50 ppm. LC₅₀and LC₉₀ were determined at 20.87 ppm and 35.27 ppm, respectively. Results indicate that Citrus hystrix leaf extracts have great potential to replace the use of chemicals as a larvicidal agent as it only requires a low concentration to kill more than 90% of the larvae. The concentration needed is much lower compared to other plants for the same purpose. Further studies are required to elucidate the bioactive compound responsible for the larval death. 2019-07 Thesis https://ir.uitm.edu.my/id/eprint/51081/ https://ir.uitm.edu.my/id/eprint/51081/1/51081.pdf text en public degree Universiti Teknologi MARA ( Kampus Puncak Alam ) Faculty of Health Sciences Camalxaman, Siti Nazrina
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Camalxaman, Siti Nazrina
topic	Bioactive compounds Diptera (Flies) Fruit and fruit culture
spellingShingle	Bioactive compounds Diptera (Flies) Fruit and fruit culture Mohamed Resli, Nik Mohamed Adzfar Larvicidal activities of citrus hystrix (kaffir lime) leaf extract against aedes albopictus / Nik Mohamed Adzfar Mohamed Resli
description	Aedes mosquitoes are widespread, and mosquito control efforts are essential to prevent outbreaks of many arboviruses such as dengue, zika and chikungunya. At present, chemical insecticides are often utilised for this purpose, which can cause long term environmental effects and the evolution of resistance if not controlled. This study was conducted to determine the efficacy of Citrus hystrix (kaffir lime) leaf extract act as a natural alternative larvicide to substitute the use of chemicals. The method of extraction used was maceration digestion. It is a modified form of maceration which involves drying, crushing, soaking with gentle heat and removal of solvent to obtain a pure crude extract. A total 25 larvae were used to test against five (5) different concentrations (50, 40, 30, 20 and 10 ppm) of Citrus hystrix for 24 - 48 hrs and evaluated in terms of mortality rates. Experimental tests were conducted in triplicate and analysed using probit analysis and analysis of variance (ANOVA). Three types of control were used in tandem namely positive (11 mg abate), negative (distilled water) and quality control (10% (v/v) solvent) to ensure the reliability of the test results. Findings revealed 100% effectiveness at 50 ppm. LC₅₀and LC₉₀ were determined at 20.87 ppm and 35.27 ppm, respectively. Results indicate that Citrus hystrix leaf extracts have great potential to replace the use of chemicals as a larvicidal agent as it only requires a low concentration to kill more than 90% of the larvae. The concentration needed is much lower compared to other plants for the same purpose. Further studies are required to elucidate the bioactive compound responsible for the larval death.
format	Thesis
qualification_level	Bachelor degree
author	Mohamed Resli, Nik Mohamed Adzfar
author_facet	Mohamed Resli, Nik Mohamed Adzfar
author_sort	Mohamed Resli, Nik Mohamed Adzfar
title	Larvicidal activities of citrus hystrix (kaffir lime) leaf extract against aedes albopictus / Nik Mohamed Adzfar Mohamed Resli
title_short	Larvicidal activities of citrus hystrix (kaffir lime) leaf extract against aedes albopictus / Nik Mohamed Adzfar Mohamed Resli
title_full	Larvicidal activities of citrus hystrix (kaffir lime) leaf extract against aedes albopictus / Nik Mohamed Adzfar Mohamed Resli
title_fullStr	Larvicidal activities of citrus hystrix (kaffir lime) leaf extract against aedes albopictus / Nik Mohamed Adzfar Mohamed Resli
title_full_unstemmed	Larvicidal activities of citrus hystrix (kaffir lime) leaf extract against aedes albopictus / Nik Mohamed Adzfar Mohamed Resli
title_sort	larvicidal activities of citrus hystrix (kaffir lime) leaf extract against aedes albopictus / nik mohamed adzfar mohamed resli
granting_institution	Universiti Teknologi MARA ( Kampus Puncak Alam )
granting_department	Faculty of Health Sciences
publishDate	2019
url	https://ir.uitm.edu.my/id/eprint/51081/1/51081.pdf
_version_	1783734842184171520

Larvicidal activities of citrus hystrix (kaffir lime) leaf extract against aedes albopictus / Nik Mohamed Adzfar Mohamed Resli

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