Antimicrobial activity of methanolic and aqueous rhodomyrtus tomentosa leaves extract against staphylococcus aureus and escherichia coli / Sarah Sorfina Mohd Sukeri

Food poisoning is one of the most risky illnesses that can affect humans or death in developing countries. Most reports are associated with bacterial contamination through the process of preparation food. Researcher nowadays attempts to discover a safer agent to use in food preparation as a food pre...

Full description

Saved in:

Bibliographic Details
Main Author:	Mohd Sukeri, Sarah Sorfina
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Bacteria
Online Access:	https://ir.uitm.edu.my/id/eprint/52131/1/52131.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uitm-ir.52131
record_format	uketd_dc
spelling	my-uitm-ir.521312021-10-12T02:56:50Z Antimicrobial activity of methanolic and aqueous rhodomyrtus tomentosa leaves extract against staphylococcus aureus and escherichia coli / Sarah Sorfina Mohd Sukeri 2019-07 Mohd Sukeri, Sarah Sorfina Bacteria Toxicology. Poisons Flowers and flower culture. Ornamental plants Food poisoning is one of the most risky illnesses that can affect humans or death in developing countries. Most reports are associated with bacterial contamination through the process of preparation food. Researcher nowadays attempts to discover a safer agent to use in food preparation as a food preservative agent, so the solvent used in extract preparation is really important. Thus, the potential of Rhodomyrtus tomentosa leaves as antimicrobial agent has been explored in this study. It was tested against two bacteria which are Staphylococcus aureus (gram positive) and Eschericia coli (gram negative). Antimicrobial activity of methanol and aqueous Rhodomyrtus tomentosa leaves extract was compared using gram staining, bacterial identification, antimicrobial sensitivity testing (AST), minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC) and statistical analysis. Disc diffusion technique was used for AST where the mean area of inhibition methanol and aqueous extract against Staphylococcus aureus was 18 mm and 16 mm respectively, whereas there is no area of inhibition for Escherichia coli. MIC is done to determine the minimum concentration that the extract need to inhibit the bacteria. After that, MBC was performed to confirm the previous MIC result. Statistical analysis shows no significant difference between methanol and aqueous extract on antimicrobial activity. In conclusion, both aqueous and methanol leaves Rhodomyrtus tomentosa yield similar antibacterial activity which prone for Staphylococcus aureus and can be used safely toward foodborne pathogen. 2019-07 Thesis https://ir.uitm.edu.my/id/eprint/52131/ https://ir.uitm.edu.my/id/eprint/52131/1/52131.pdf text en public degree Universiti Teknologi MARA ( Kampus Puncak Alam ) Faculty of Health Sciences Bahari, Mazura
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Bahari, Mazura
topic	Bacteria Bacteria Bacteria
spellingShingle	Bacteria Bacteria Bacteria Mohd Sukeri, Sarah Sorfina Antimicrobial activity of methanolic and aqueous rhodomyrtus tomentosa leaves extract against staphylococcus aureus and escherichia coli / Sarah Sorfina Mohd Sukeri
description	Food poisoning is one of the most risky illnesses that can affect humans or death in developing countries. Most reports are associated with bacterial contamination through the process of preparation food. Researcher nowadays attempts to discover a safer agent to use in food preparation as a food preservative agent, so the solvent used in extract preparation is really important. Thus, the potential of Rhodomyrtus tomentosa leaves as antimicrobial agent has been explored in this study. It was tested against two bacteria which are Staphylococcus aureus (gram positive) and Eschericia coli (gram negative). Antimicrobial activity of methanol and aqueous Rhodomyrtus tomentosa leaves extract was compared using gram staining, bacterial identification, antimicrobial sensitivity testing (AST), minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC) and statistical analysis. Disc diffusion technique was used for AST where the mean area of inhibition methanol and aqueous extract against Staphylococcus aureus was 18 mm and 16 mm respectively, whereas there is no area of inhibition for Escherichia coli. MIC is done to determine the minimum concentration that the extract need to inhibit the bacteria. After that, MBC was performed to confirm the previous MIC result. Statistical analysis shows no significant difference between methanol and aqueous extract on antimicrobial activity. In conclusion, both aqueous and methanol leaves Rhodomyrtus tomentosa yield similar antibacterial activity which prone for Staphylococcus aureus and can be used safely toward foodborne pathogen.
format	Thesis
qualification_level	Bachelor degree
author	Mohd Sukeri, Sarah Sorfina
author_facet	Mohd Sukeri, Sarah Sorfina
author_sort	Mohd Sukeri, Sarah Sorfina
title	Antimicrobial activity of methanolic and aqueous rhodomyrtus tomentosa leaves extract against staphylococcus aureus and escherichia coli / Sarah Sorfina Mohd Sukeri
title_short	Antimicrobial activity of methanolic and aqueous rhodomyrtus tomentosa leaves extract against staphylococcus aureus and escherichia coli / Sarah Sorfina Mohd Sukeri
title_full	Antimicrobial activity of methanolic and aqueous rhodomyrtus tomentosa leaves extract against staphylococcus aureus and escherichia coli / Sarah Sorfina Mohd Sukeri
title_fullStr	Antimicrobial activity of methanolic and aqueous rhodomyrtus tomentosa leaves extract against staphylococcus aureus and escherichia coli / Sarah Sorfina Mohd Sukeri
title_full_unstemmed	Antimicrobial activity of methanolic and aqueous rhodomyrtus tomentosa leaves extract against staphylococcus aureus and escherichia coli / Sarah Sorfina Mohd Sukeri
title_sort	antimicrobial activity of methanolic and aqueous rhodomyrtus tomentosa leaves extract against staphylococcus aureus and escherichia coli / sarah sorfina mohd sukeri
granting_institution	Universiti Teknologi MARA ( Kampus Puncak Alam )
granting_department	Faculty of Health Sciences
publishDate	2019
url	https://ir.uitm.edu.my/id/eprint/52131/1/52131.pdf
_version_	1783734845340385280

Antimicrobial activity of methanolic and aqueous rhodomyrtus tomentosa leaves extract against staphylococcus aureus and escherichia coli / Sarah Sorfina Mohd Sukeri

Similar Items