Optimization of Keli Afrika (Clarias gariepnus) hydrolysate production by alcalase: effect of pH and temperature / Umi Amirah Mohamad.

Fish protein hydrolysate (FPH) was produced from 'Keli' an African catfish (Clarias gariepinus) which is one of the types of freshwater fish widely farm in Malaysia. The production for this hydrolysate was using Alcalase which is one of the protease enzymes. Hydrolysis conditions for prepa...

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Main Author:	Mohamad, Umi Amirah
Format:	Thesis
Language:	English
Published:	2009
Subjects:	Hydrolysis Biotechnology
Online Access:	https://ir.uitm.edu.my/id/eprint/52974/1/52974.pdf
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id	my-uitm-ir.52974
record_format	uketd_dc
spelling	my-uitm-ir.529742021-12-22T08:18:41Z Optimization of Keli Afrika (Clarias gariepnus) hydrolysate production by alcalase: effect of pH and temperature / Umi Amirah Mohamad. 2009 Mohamad, Umi Amirah Hydrolysis Biotechnology Fish protein hydrolysate (FPH) was produced from 'Keli' an African catfish (Clarias gariepinus) which is one of the types of freshwater fish widely farm in Malaysia. The production for this hydrolysate was using Alcalase which is one of the protease enzymes. Hydrolysis conditions for preparing hydrolysate from the fish protein were optimized by response surface methodology (RSM) by employing a factorial design. The smaller and less time consuming experimental design could generally suffice for the optimization of many processes. The model equations were proposed with regard to the effects of temperature (T) and pH on the degree of hydrolysis (DH) and degree of nitrogen recovery (NR). An enzyme to substrate level of 2% (v/w), hydrolysis times of 2 hours with temperature and pH that have bear suggested by RSM were found to be the maximum condition to obtain a higher degree of hydrolysis at 78.28°C and pH 7 was 8.425% and for degree of nitrogen recovery at 70°C and pH 4 was 0.898%. In the optimization of 'Keli Afrika' hydrolysate production by Alcalase, there is no significant (p > 0.05) effect of temperature and pH on percentage of degree of hydrolysis and percentage of nitrogen recovery. 2009 Thesis https://ir.uitm.edu.my/id/eprint/52974/ https://ir.uitm.edu.my/id/eprint/52974/1/52974.pdf text en public degree Universiti Teknologi MARA Faculty of Applied Sciences Ismail, Normah (Dr.)
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Ismail, Normah (Dr.)
topic	Hydrolysis Biotechnology
spellingShingle	Hydrolysis Biotechnology Mohamad, Umi Amirah Optimization of Keli Afrika (Clarias gariepnus) hydrolysate production by alcalase: effect of pH and temperature / Umi Amirah Mohamad.
description	Fish protein hydrolysate (FPH) was produced from 'Keli' an African catfish (Clarias gariepinus) which is one of the types of freshwater fish widely farm in Malaysia. The production for this hydrolysate was using Alcalase which is one of the protease enzymes. Hydrolysis conditions for preparing hydrolysate from the fish protein were optimized by response surface methodology (RSM) by employing a factorial design. The smaller and less time consuming experimental design could generally suffice for the optimization of many processes. The model equations were proposed with regard to the effects of temperature (T) and pH on the degree of hydrolysis (DH) and degree of nitrogen recovery (NR). An enzyme to substrate level of 2% (v/w), hydrolysis times of 2 hours with temperature and pH that have bear suggested by RSM were found to be the maximum condition to obtain a higher degree of hydrolysis at 78.28°C and pH 7 was 8.425% and for degree of nitrogen recovery at 70°C and pH 4 was 0.898%. In the optimization of 'Keli Afrika' hydrolysate production by Alcalase, there is no significant (p > 0.05) effect of temperature and pH on percentage of degree of hydrolysis and percentage of nitrogen recovery.
format	Thesis
qualification_level	Bachelor degree
author	Mohamad, Umi Amirah
author_facet	Mohamad, Umi Amirah
author_sort	Mohamad, Umi Amirah
title	Optimization of Keli Afrika (Clarias gariepnus) hydrolysate production by alcalase: effect of pH and temperature / Umi Amirah Mohamad.
title_short	Optimization of Keli Afrika (Clarias gariepnus) hydrolysate production by alcalase: effect of pH and temperature / Umi Amirah Mohamad.
title_full	Optimization of Keli Afrika (Clarias gariepnus) hydrolysate production by alcalase: effect of pH and temperature / Umi Amirah Mohamad.
title_fullStr	Optimization of Keli Afrika (Clarias gariepnus) hydrolysate production by alcalase: effect of pH and temperature / Umi Amirah Mohamad.
title_full_unstemmed	Optimization of Keli Afrika (Clarias gariepnus) hydrolysate production by alcalase: effect of pH and temperature / Umi Amirah Mohamad.
title_sort	optimization of keli afrika (clarias gariepnus) hydrolysate production by alcalase: effect of ph and temperature / umi amirah mohamad.
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2009
url	https://ir.uitm.edu.my/id/eprint/52974/1/52974.pdf
_version_	1783734852553539584

Optimization of Keli Afrika (Clarias gariepnus) hydrolysate production by alcalase: effect of pH and temperature / Umi Amirah Mohamad.

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