Evaluation on the degree of bitterness of green mussel (Perna viridis) protein hydrolysate / Siti Hafsah Mohd Shah

Properties of protein hydrolysate prepared from green mussel (Perna viridis) by enzymatic hydrolysis using Alcalase® 2.4L at different pH and enzyme to substrate ratio were determined. Hydrolysis was performed using the pH-stat method. Mussel hydrolysate produced at pH 7 with E/S 5% had protein cont...

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Main Author: Mohd Shah, Siti Hafsah
Format: Thesis
Language:English
Published: 2012
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/52976/1/52976.pdf
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spelling my-uitm-ir.529762021-12-22T06:48:08Z Evaluation on the degree of bitterness of green mussel (Perna viridis) protein hydrolysate / Siti Hafsah Mohd Shah 2012 Mohd Shah, Siti Hafsah Biochemistry Properties of protein hydrolysate prepared from green mussel (Perna viridis) by enzymatic hydrolysis using Alcalase® 2.4L at different pH and enzyme to substrate ratio were determined. Hydrolysis was performed using the pH-stat method. Mussel hydrolysate produced at pH 7 with E/S 5% had protein content of 37.17%. Mussel hydrolysate produced at pH 7 had low molecular weight peptide which was < 18 kDa. It also contain high amount of Methionine, Phenylalanine and Proline which contributed to bitter taste. Mussel hydrolysate produced at pH 7 was the most acceptable and the degree of bitterness was slightly bitter but not exceeded the bitterness of the standard caffeine. These results suggested that green mussel (Perna viridis) hydrolysates have the potential to be applied as a flavoring agent in the food since the bitterness is still in acceptable range. 2012 Thesis https://ir.uitm.edu.my/id/eprint/52976/ https://ir.uitm.edu.my/id/eprint/52976/1/52976.pdf text en public degree Universiti Teknologi MARA Faculty of Applied Sciences
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
topic Biochemistry
spellingShingle Biochemistry
Mohd Shah, Siti Hafsah
Evaluation on the degree of bitterness of green mussel (Perna viridis) protein hydrolysate / Siti Hafsah Mohd Shah
description Properties of protein hydrolysate prepared from green mussel (Perna viridis) by enzymatic hydrolysis using Alcalase® 2.4L at different pH and enzyme to substrate ratio were determined. Hydrolysis was performed using the pH-stat method. Mussel hydrolysate produced at pH 7 with E/S 5% had protein content of 37.17%. Mussel hydrolysate produced at pH 7 had low molecular weight peptide which was < 18 kDa. It also contain high amount of Methionine, Phenylalanine and Proline which contributed to bitter taste. Mussel hydrolysate produced at pH 7 was the most acceptable and the degree of bitterness was slightly bitter but not exceeded the bitterness of the standard caffeine. These results suggested that green mussel (Perna viridis) hydrolysates have the potential to be applied as a flavoring agent in the food since the bitterness is still in acceptable range.
format Thesis
qualification_level Bachelor degree
author Mohd Shah, Siti Hafsah
author_facet Mohd Shah, Siti Hafsah
author_sort Mohd Shah, Siti Hafsah
title Evaluation on the degree of bitterness of green mussel (Perna viridis) protein hydrolysate / Siti Hafsah Mohd Shah
title_short Evaluation on the degree of bitterness of green mussel (Perna viridis) protein hydrolysate / Siti Hafsah Mohd Shah
title_full Evaluation on the degree of bitterness of green mussel (Perna viridis) protein hydrolysate / Siti Hafsah Mohd Shah
title_fullStr Evaluation on the degree of bitterness of green mussel (Perna viridis) protein hydrolysate / Siti Hafsah Mohd Shah
title_full_unstemmed Evaluation on the degree of bitterness of green mussel (Perna viridis) protein hydrolysate / Siti Hafsah Mohd Shah
title_sort evaluation on the degree of bitterness of green mussel (perna viridis) protein hydrolysate / siti hafsah mohd shah
granting_institution Universiti Teknologi MARA
granting_department Faculty of Applied Sciences
publishDate 2012
url https://ir.uitm.edu.my/id/eprint/52976/1/52976.pdf
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