Optimization of hydrolysis conditions for the production of shrimp hydrolysate by using protamex: effect of enzyme substrate (ES) ratio and time / Nurul Nadiah Ahmad Latif.

The optimization of hydrolysis conditions which are enzyme substrate ratio ..and hydrolysis time for the production of shrimp hydrolysate by using protamex were studied. This study was done to obtain the optimum percentage for nitrogen recovery and degree of hydrolysis on the hydrolysis conditions....

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Main Author:	Ahmad Latif, Nurul Nadiah
Format:	Thesis
Language:	English
Published:	2009
Subjects:	Chemical engineering Hydrolysis Biotechnology
Online Access:	https://ir.uitm.edu.my/id/eprint/53000/1/53000.pdf
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id	my-uitm-ir.53000
record_format	uketd_dc
spelling	my-uitm-ir.530002021-12-23T04:48:56Z Optimization of hydrolysis conditions for the production of shrimp hydrolysate by using protamex: effect of enzyme substrate (ES) ratio and time / Nurul Nadiah Ahmad Latif. 2009 Ahmad Latif, Nurul Nadiah Chemical engineering Hydrolysis Biotechnology The optimization of hydrolysis conditions which are enzyme substrate ratio ..and hydrolysis time for the production of shrimp hydrolysate by using protamex were studied. This study was done to obtain the optimum percentage for nitrogen recovery and degree of hydrolysis on the hydrolysis conditions. Methods for production of shrimp hydrolysate include homogenization, adjustment of pH and temperature, addition of enzyme and hydrolysis time which are based on response surface methodology (RSM) suggestion, termination of hydrolysis, cooled and centrifuged the hydrolysis sample, collection of supernatant and analysis for percentage of nitrogen recovery and degree of hydrolysis. Total nitrogen in supernatant was determined by using Kjeldhal method. The percentage of degree of hydrolysis was determined by using modified formol titration method. The data that were obtained from the experiment were accomplished by employing the RSM using Design Expert 4 Software. By fitting the experimental data to the equation, the percentage nitrogen recovery (%NR) optimum levels for time (240 min), ES ratio (0%), (%NR) (3.90308) with desirability (0.657) at constant pH 6 and constant temperature 60°C were determined. For percentage degree of hydrolysis (%DH), the optimum levels for time (0 min), ES ratio (4%), %DH (2.80186) with desirability (0.693) were determined. 2009 Thesis https://ir.uitm.edu.my/id/eprint/53000/ https://ir.uitm.edu.my/id/eprint/53000/1/53000.pdf text en public degree Universiti Teknologi MARA Faculty of Applied Sciences Ismail, Normah (Dr.)
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Ismail, Normah (Dr.)
topic	Chemical engineering Hydrolysis Biotechnology
spellingShingle	Chemical engineering Hydrolysis Biotechnology Ahmad Latif, Nurul Nadiah Optimization of hydrolysis conditions for the production of shrimp hydrolysate by using protamex: effect of enzyme substrate (ES) ratio and time / Nurul Nadiah Ahmad Latif.
description	The optimization of hydrolysis conditions which are enzyme substrate ratio ..and hydrolysis time for the production of shrimp hydrolysate by using protamex were studied. This study was done to obtain the optimum percentage for nitrogen recovery and degree of hydrolysis on the hydrolysis conditions. Methods for production of shrimp hydrolysate include homogenization, adjustment of pH and temperature, addition of enzyme and hydrolysis time which are based on response surface methodology (RSM) suggestion, termination of hydrolysis, cooled and centrifuged the hydrolysis sample, collection of supernatant and analysis for percentage of nitrogen recovery and degree of hydrolysis. Total nitrogen in supernatant was determined by using Kjeldhal method. The percentage of degree of hydrolysis was determined by using modified formol titration method. The data that were obtained from the experiment were accomplished by employing the RSM using Design Expert 4 Software. By fitting the experimental data to the equation, the percentage nitrogen recovery (%NR) optimum levels for time (240 min), ES ratio (0%), (%NR) (3.90308) with desirability (0.657) at constant pH 6 and constant temperature 60°C were determined. For percentage degree of hydrolysis (%DH), the optimum levels for time (0 min), ES ratio (4%), %DH (2.80186) with desirability (0.693) were determined.
format	Thesis
qualification_level	Bachelor degree
author	Ahmad Latif, Nurul Nadiah
author_facet	Ahmad Latif, Nurul Nadiah
author_sort	Ahmad Latif, Nurul Nadiah
title	Optimization of hydrolysis conditions for the production of shrimp hydrolysate by using protamex: effect of enzyme substrate (ES) ratio and time / Nurul Nadiah Ahmad Latif.
title_short	Optimization of hydrolysis conditions for the production of shrimp hydrolysate by using protamex: effect of enzyme substrate (ES) ratio and time / Nurul Nadiah Ahmad Latif.
title_full	Optimization of hydrolysis conditions for the production of shrimp hydrolysate by using protamex: effect of enzyme substrate (ES) ratio and time / Nurul Nadiah Ahmad Latif.
title_fullStr	Optimization of hydrolysis conditions for the production of shrimp hydrolysate by using protamex: effect of enzyme substrate (ES) ratio and time / Nurul Nadiah Ahmad Latif.
title_full_unstemmed	Optimization of hydrolysis conditions for the production of shrimp hydrolysate by using protamex: effect of enzyme substrate (ES) ratio and time / Nurul Nadiah Ahmad Latif.
title_sort	optimization of hydrolysis conditions for the production of shrimp hydrolysate by using protamex: effect of enzyme substrate (es) ratio and time / nurul nadiah ahmad latif.
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2009
url	https://ir.uitm.edu.my/id/eprint/53000/1/53000.pdf
_version_	1783734855625867264

Optimization of hydrolysis conditions for the production of shrimp hydrolysate by using protamex: effect of enzyme substrate (ES) ratio and time / Nurul Nadiah Ahmad Latif.

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