Optimization of the hydrolysis conditions (effect of temperature and pH) for the production of "udang geragau' (Acetes japonicus) hydrolysate / Noor Afiqah Muhammad Basri.

Response surface methodology (RSM) was used to optimize the hydrolysis parameters of Acetes japonicus by Alcalase 2.4L in order to obtain a hydrolysate. A Central Composite design by Design Expert 7.0.3 software created 14 runs with different conditions of parameters. The parameters were temperature...

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Main Author: Muhammad Basri, Noor Afiqah
Format: Thesis
Language:English
Published: 2011
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/53013/1/53013.pdf
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spelling my-uitm-ir.530132021-12-22T06:04:15Z Optimization of the hydrolysis conditions (effect of temperature and pH) for the production of "udang geragau' (Acetes japonicus) hydrolysate / Noor Afiqah Muhammad Basri. 2011 Muhammad Basri, Noor Afiqah Biochemistry Extraction (Chemistry) Response surface methodology (RSM) was used to optimize the hydrolysis parameters of Acetes japonicus by Alcalase 2.4L in order to obtain a hydrolysate. A Central Composite design by Design Expert 7.0.3 software created 14 runs with different conditions of parameters. The parameters were temperature and pH with percentage of nitrogen recovery (NR) and degree of hydrolysis (DH) being the response. While the constant variable is the time spent was 120 minutes and the enzyme concentration was 2% for each trial. The results showed that the optimum condition of % nitrogen recovery for Acetes japonicus hydrolysate about 4.025 % with pH 8 at 45 °C meanwhile percentage of degree of hydrolysis about 4.025 % at pH 6 and temperature 45.19 °C. Temperature and pH were the effective factors on the yield of hydrolysate. 2011 Thesis https://ir.uitm.edu.my/id/eprint/53013/ https://ir.uitm.edu.my/id/eprint/53013/1/53013.pdf text en public degree Universiti Teknologi MARA Faculty of Applied Sciences
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
topic Biochemistry
Extraction (Chemistry)
spellingShingle Biochemistry
Extraction (Chemistry)
Muhammad Basri, Noor Afiqah
Optimization of the hydrolysis conditions (effect of temperature and pH) for the production of "udang geragau' (Acetes japonicus) hydrolysate / Noor Afiqah Muhammad Basri.
description Response surface methodology (RSM) was used to optimize the hydrolysis parameters of Acetes japonicus by Alcalase 2.4L in order to obtain a hydrolysate. A Central Composite design by Design Expert 7.0.3 software created 14 runs with different conditions of parameters. The parameters were temperature and pH with percentage of nitrogen recovery (NR) and degree of hydrolysis (DH) being the response. While the constant variable is the time spent was 120 minutes and the enzyme concentration was 2% for each trial. The results showed that the optimum condition of % nitrogen recovery for Acetes japonicus hydrolysate about 4.025 % with pH 8 at 45 °C meanwhile percentage of degree of hydrolysis about 4.025 % at pH 6 and temperature 45.19 °C. Temperature and pH were the effective factors on the yield of hydrolysate.
format Thesis
qualification_level Bachelor degree
author Muhammad Basri, Noor Afiqah
author_facet Muhammad Basri, Noor Afiqah
author_sort Muhammad Basri, Noor Afiqah
title Optimization of the hydrolysis conditions (effect of temperature and pH) for the production of "udang geragau' (Acetes japonicus) hydrolysate / Noor Afiqah Muhammad Basri.
title_short Optimization of the hydrolysis conditions (effect of temperature and pH) for the production of "udang geragau' (Acetes japonicus) hydrolysate / Noor Afiqah Muhammad Basri.
title_full Optimization of the hydrolysis conditions (effect of temperature and pH) for the production of "udang geragau' (Acetes japonicus) hydrolysate / Noor Afiqah Muhammad Basri.
title_fullStr Optimization of the hydrolysis conditions (effect of temperature and pH) for the production of "udang geragau' (Acetes japonicus) hydrolysate / Noor Afiqah Muhammad Basri.
title_full_unstemmed Optimization of the hydrolysis conditions (effect of temperature and pH) for the production of "udang geragau' (Acetes japonicus) hydrolysate / Noor Afiqah Muhammad Basri.
title_sort optimization of the hydrolysis conditions (effect of temperature and ph) for the production of "udang geragau' (acetes japonicus) hydrolysate / noor afiqah muhammad basri.
granting_institution Universiti Teknologi MARA
granting_department Faculty of Applied Sciences
publishDate 2011
url https://ir.uitm.edu.my/id/eprint/53013/1/53013.pdf
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