Optimization of Keli Afrika (clarias gariepinus) hydrolysate by alcalase (effect of time and enzymes substrate ratio) / Zamri Idris
The optimization of catfish hydrolysate by Alcalase was obtained from the experiment at optimum condition 137 minutes and 4% enzymes concentration with temperature 50° C and pH 7 as determined by randomized surface methodology (RSM). Whereby, the nitrogen recovered from catfish hydrolysis was optimu...
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| Format: | Thesis |
| Language: | English |
| Published: |
2009
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| Subjects: | |
| Online Access: | https://ir.uitm.edu.my/id/eprint/53054/1/53054.pdf |
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| Summary: | The optimization of catfish hydrolysate by Alcalase was obtained from the experiment at optimum condition 137 minutes and 4% enzymes concentration with temperature 50° C and pH 7 as determined by randomized surface methodology (RSM). Whereby, the nitrogen recovered from catfish hydrolysis was optimum condition at 147 minutes and 4% enzymes concentration. The degree hydrolysis of hydrolysate was 4.11 % for catfish flesh were treated with enzymes Alcalase. The total nitrogen content of enzymatic hydrolysate range from 0.85% to 2.86%.the characteristic of the hydrolysate was obtained from hydrolysis was brown in color with sticky characteristic. Increasing the time and enzymes concentration significantly (p<0.05) increased the degree of hydrolysis. |
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