Characterization and purification of protease extracted from "noni" (Morinda Citrifolia L.) at two maturity stages / Nurulain Abd Razak.

Protease was extracted from two maturity stages of noni fruits (Morinda citrifolia L.), unripe (stage 1) and ripe (stage 5) and then purified using acetone precipitation, dialysis, gel filtration (Sephadex G-25) and freeze drying. Protease obtained from each of purification step "was analysed f...

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Main Author:	Abd Razak, Nurulain
Format:	Thesis
Language:	English
Published:	2014
Subjects:	Plant anatomy
Online Access:	https://ir.uitm.edu.my/id/eprint/53058/2/53058.pdf
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id	my-uitm-ir.53058
record_format	uketd_dc
spelling	my-uitm-ir.530582021-12-14T08:56:29Z Characterization and purification of protease extracted from "noni" (Morinda Citrifolia L.) at two maturity stages / Nurulain Abd Razak. 2014 Abd Razak, Nurulain Plant anatomy Growth. Development. Including pattern formation Botanical chemistry. Phytochemicals Protease was extracted from two maturity stages of noni fruits (Morinda citrifolia L.), unripe (stage 1) and ripe (stage 5) and then purified using acetone precipitation, dialysis, gel filtration (Sephadex G-25) and freeze drying. Protease obtained from each of purification step "was analysed for protein concentrations, proteolytic activity, yield and molecular weight distribution (SDS-PAGE). Freeze dried protease were analysed for pH stability, temperature stability and storage efficiency. Protein content in unripe (stage 1) noni fruit was higher than those in the ripe (stage 5) fruit. Protein concentration for protease for unripe (stage 1) noni fuit was higher for protease purified from crude extract until dialysis step. Molecular weight distribution of purified proteases for both stages was approximately at 3 to 28 kDa. The optimum activity of purified proteases was at pH 7 and 6 at 40 and 50 °C for unripe (stage 1) and ripe (stage 5) noni fruit respectively. Protease from noni fruit could be an alternative source of plant protease based "on its high proteolytic activity and stability during storage. 2014 Thesis https://ir.uitm.edu.my/id/eprint/53058/ https://ir.uitm.edu.my/id/eprint/53058/2/53058.pdf text en public degree Universiti Teknologi MARA Faculty of Applied Sciences Ismail, Normah (Dr.)
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Ismail, Normah (Dr.)
topic	Plant anatomy Plant anatomy Plant anatomy
spellingShingle	Plant anatomy Plant anatomy Plant anatomy Abd Razak, Nurulain Characterization and purification of protease extracted from "noni" (Morinda Citrifolia L.) at two maturity stages / Nurulain Abd Razak.
description	Protease was extracted from two maturity stages of noni fruits (Morinda citrifolia L.), unripe (stage 1) and ripe (stage 5) and then purified using acetone precipitation, dialysis, gel filtration (Sephadex G-25) and freeze drying. Protease obtained from each of purification step "was analysed for protein concentrations, proteolytic activity, yield and molecular weight distribution (SDS-PAGE). Freeze dried protease were analysed for pH stability, temperature stability and storage efficiency. Protein content in unripe (stage 1) noni fruit was higher than those in the ripe (stage 5) fruit. Protein concentration for protease for unripe (stage 1) noni fuit was higher for protease purified from crude extract until dialysis step. Molecular weight distribution of purified proteases for both stages was approximately at 3 to 28 kDa. The optimum activity of purified proteases was at pH 7 and 6 at 40 and 50 °C for unripe (stage 1) and ripe (stage 5) noni fruit respectively. Protease from noni fruit could be an alternative source of plant protease based "on its high proteolytic activity and stability during storage.
format	Thesis
qualification_level	Bachelor degree
author	Abd Razak, Nurulain
author_facet	Abd Razak, Nurulain
author_sort	Abd Razak, Nurulain
title	Characterization and purification of protease extracted from "noni" (Morinda Citrifolia L.) at two maturity stages / Nurulain Abd Razak.
title_short	Characterization and purification of protease extracted from "noni" (Morinda Citrifolia L.) at two maturity stages / Nurulain Abd Razak.
title_full	Characterization and purification of protease extracted from "noni" (Morinda Citrifolia L.) at two maturity stages / Nurulain Abd Razak.
title_fullStr	Characterization and purification of protease extracted from "noni" (Morinda Citrifolia L.) at two maturity stages / Nurulain Abd Razak.
title_full_unstemmed	Characterization and purification of protease extracted from "noni" (Morinda Citrifolia L.) at two maturity stages / Nurulain Abd Razak.
title_sort	characterization and purification of protease extracted from "noni" (morinda citrifolia l.) at two maturity stages / nurulain abd razak.
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2014
url	https://ir.uitm.edu.my/id/eprint/53058/2/53058.pdf
_version_	1783734858967678976

Characterization and purification of protease extracted from "noni" (Morinda Citrifolia L.) at two maturity stages / Nurulain Abd Razak.

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