Extraction and characterization of collagen extracted from the waste of sin croaker (Johniecop sina) / Maidzatul Afiqah Mahadzir

Fish waste is major by-product of the fish processing industry, causing wastage and pollution, thus utilizing the waste of sin croaker which is mainly used „ in the fish processing industry could provide a valuable source of collagen. The primary objective of this study was to determine the yield of...

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Main Author:	Mahadzir, Maidzatul Afiqah
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Extraction (Chemistry)
Online Access:	https://ir.uitm.edu.my/id/eprint/53076/1/53076.pdf
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id	my-uitm-ir.53076
record_format	uketd_dc
spelling	my-uitm-ir.530762021-12-15T07:42:50Z Extraction and characterization of collagen extracted from the waste of sin croaker (Johniecop sina) / Maidzatul Afiqah Mahadzir 2015 Mahadzir, Maidzatul Afiqah Extraction (Chemistry) Fish waste is major by-product of the fish processing industry, causing wastage and pollution, thus utilizing the waste of sin croaker which is mainly used „ in the fish processing industry could provide a valuable source of collagen. The primary objective of this study was to determine the yield of collagen and physicochemical characteristic of collagen sin croaker waste at different duration of extraction time which is three and five days. The extracted collagen were also compared with the commercial collagen. Collagen was extracted from the waste of sin croaker using acid solubilisation method. The yields percentages obtained from the three and five days of extraction were 2.74 and 3.36% respectively. Both collagen was most soluble in low pH (pH 1-2) and low salt concentration (2% NaCl). Diffrential Scanning Calorimetry (DSC) and viscoity measurement were used to determine the thermal stability of the collagen. The extracted collagen for three and five days showed good thermal stability as it denatured at 31.31 and 32.10°C for the three and five days of extraction, respectively. SDS-PAGE indicated that both collagens are type I collagen as they contain al, a2 chains, P and y components. As a conclusion, collagen extracted for five days are better than three days due to high yields and higher thermal stability. 2015 Thesis https://ir.uitm.edu.my/id/eprint/53076/ https://ir.uitm.edu.my/id/eprint/53076/1/53076.pdf text en public degree Universiti Teknologi MARA Faculty of Applied Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Extraction (Chemistry)
spellingShingle	Extraction (Chemistry) Mahadzir, Maidzatul Afiqah Extraction and characterization of collagen extracted from the waste of sin croaker (Johniecop sina) / Maidzatul Afiqah Mahadzir
description	Fish waste is major by-product of the fish processing industry, causing wastage and pollution, thus utilizing the waste of sin croaker which is mainly used „ in the fish processing industry could provide a valuable source of collagen. The primary objective of this study was to determine the yield of collagen and physicochemical characteristic of collagen sin croaker waste at different duration of extraction time which is three and five days. The extracted collagen were also compared with the commercial collagen. Collagen was extracted from the waste of sin croaker using acid solubilisation method. The yields percentages obtained from the three and five days of extraction were 2.74 and 3.36% respectively. Both collagen was most soluble in low pH (pH 1-2) and low salt concentration (2% NaCl). Diffrential Scanning Calorimetry (DSC) and viscoity measurement were used to determine the thermal stability of the collagen. The extracted collagen for three and five days showed good thermal stability as it denatured at 31.31 and 32.10°C for the three and five days of extraction, respectively. SDS-PAGE indicated that both collagens are type I collagen as they contain al, a2 chains, P and y components. As a conclusion, collagen extracted for five days are better than three days due to high yields and higher thermal stability.
format	Thesis
qualification_level	Bachelor degree
author	Mahadzir, Maidzatul Afiqah
author_facet	Mahadzir, Maidzatul Afiqah
author_sort	Mahadzir, Maidzatul Afiqah
title	Extraction and characterization of collagen extracted from the waste of sin croaker (Johniecop sina) / Maidzatul Afiqah Mahadzir
title_short	Extraction and characterization of collagen extracted from the waste of sin croaker (Johniecop sina) / Maidzatul Afiqah Mahadzir
title_full	Extraction and characterization of collagen extracted from the waste of sin croaker (Johniecop sina) / Maidzatul Afiqah Mahadzir
title_fullStr	Extraction and characterization of collagen extracted from the waste of sin croaker (Johniecop sina) / Maidzatul Afiqah Mahadzir
title_full_unstemmed	Extraction and characterization of collagen extracted from the waste of sin croaker (Johniecop sina) / Maidzatul Afiqah Mahadzir
title_sort	extraction and characterization of collagen extracted from the waste of sin croaker (johniecop sina) / maidzatul afiqah mahadzir
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2015
url	https://ir.uitm.edu.my/id/eprint/53076/1/53076.pdf
_version_	1783734860899155968

Extraction and characterization of collagen extracted from the waste of sin croaker (Johniecop sina) / Maidzatul Afiqah Mahadzir

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