Biodegradation of naphthalene in a soil medium using Pleurotus ostreatus with the addition of biomass as a nutrient / Mohd Zaki Sukor

The efficiency and kinetics of naphthalene biodegradation in a soil medium using Pleurotus ostreatus (a type of white rot fungus) with and without the addition of Oil Palm Fiber (OPF) as a nutrient are evaluated in this study. For this purpose, Pleurotus ostreatus mycelium is specifically cultured i...

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Main Author:	Sukor, Mohd Zaki
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Biotechnology Biomass
Online Access:	https://ir.uitm.edu.my/id/eprint/54140/1/54140.pdf
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id	my-uitm-ir.54140
record_format	uketd_dc
spelling	my-uitm-ir.541402023-07-21T00:26:06Z Biodegradation of naphthalene in a soil medium using Pleurotus ostreatus with the addition of biomass as a nutrient / Mohd Zaki Sukor 2012 Sukor, Mohd Zaki Biotechnology Biomass The efficiency and kinetics of naphthalene biodegradation in a soil medium using Pleurotus ostreatus (a type of white rot fungus) with and without the addition of Oil Palm Fiber (OPF) as a nutrient are evaluated in this study. For this purpose, Pleurotus ostreatus mycelium is specifically cultured in potato dextrose agar for 13 days prior to biodegradation. Three batches have been used in the biodegradation studies; (i) control-spiked soil, (ii) spiked soil with fungus, and (hi) spiked soil with both fungus and OPF. Biodegradation is conducted over a period of 22 days during which naphthalene is extracted from the soil using microwave extraction and its concentration determined using high-performance liquid chromatography. The results for the first part of the study indicate that the inoculation of Pleurotus ostreatus without and with the addition of OPF significantly enhances the rate of naphthalene biodegradation in contaminated soil by 76 and 117%, respectively. The high carbon content in OPF (> 40%) affords it to be a viable nutrient supplement for Pleurotus ostreatus and consequently indicates that it has the potential to be used in the biodegradation of other Polycyclic Aromatic Hydrocarbons (PAHs). The biodegradation kinetics data presented in this study may be used to scale-up existing laboratory-scale PAH biodegradation systems tailored for either in-situ or ex-situ contaminated soil remediation applications. In the second part of the study, the effect of the addition of different quantities of nutrient mass in the bioremediation process has been investigated. Four batches have been used, which constitute the addition of 0.5, 1, 2 and 3 g of OPF to each batch. The rate of biodegradation increases from 0.165 day-1 to 0.176 day-1 upon the addition of 1.0 g of OPF, however the highest biodegradation rate, 0.257 day-1, is observed upon the addition of 3.0 g of OPF and exhibits a liner relationship between the rate of biodegradation and the mass of OPF added. A relationship has been determined with respect to the biodegradation rate constant of the form: k = 0.0299 x OPF + 0.1509 with a correlation of determination value of 0.8191, which indicates that the data is well-described. This simple correlation may be used in order to perform quick estimation of the amount of OPF required to achieve specific biodegradation rates. 2012 Thesis https://ir.uitm.edu.my/id/eprint/54140/ https://ir.uitm.edu.my/id/eprint/54140/1/54140.pdf text en public masters Universiti Teknologi MARA Faculty of Art and Design
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Biotechnology Biomass
spellingShingle	Biotechnology Biomass Sukor, Mohd Zaki Biodegradation of naphthalene in a soil medium using Pleurotus ostreatus with the addition of biomass as a nutrient / Mohd Zaki Sukor
description	The efficiency and kinetics of naphthalene biodegradation in a soil medium using Pleurotus ostreatus (a type of white rot fungus) with and without the addition of Oil Palm Fiber (OPF) as a nutrient are evaluated in this study. For this purpose, Pleurotus ostreatus mycelium is specifically cultured in potato dextrose agar for 13 days prior to biodegradation. Three batches have been used in the biodegradation studies; (i) control-spiked soil, (ii) spiked soil with fungus, and (hi) spiked soil with both fungus and OPF. Biodegradation is conducted over a period of 22 days during which naphthalene is extracted from the soil using microwave extraction and its concentration determined using high-performance liquid chromatography. The results for the first part of the study indicate that the inoculation of Pleurotus ostreatus without and with the addition of OPF significantly enhances the rate of naphthalene biodegradation in contaminated soil by 76 and 117%, respectively. The high carbon content in OPF (> 40%) affords it to be a viable nutrient supplement for Pleurotus ostreatus and consequently indicates that it has the potential to be used in the biodegradation of other Polycyclic Aromatic Hydrocarbons (PAHs). The biodegradation kinetics data presented in this study may be used to scale-up existing laboratory-scale PAH biodegradation systems tailored for either in-situ or ex-situ contaminated soil remediation applications. In the second part of the study, the effect of the addition of different quantities of nutrient mass in the bioremediation process has been investigated. Four batches have been used, which constitute the addition of 0.5, 1, 2 and 3 g of OPF to each batch. The rate of biodegradation increases from 0.165 day-1 to 0.176 day-1 upon the addition of 1.0 g of OPF, however the highest biodegradation rate, 0.257 day-1, is observed upon the addition of 3.0 g of OPF and exhibits a liner relationship between the rate of biodegradation and the mass of OPF added. A relationship has been determined with respect to the biodegradation rate constant of the form: k = 0.0299 x OPF + 0.1509 with a correlation of determination value of 0.8191, which indicates that the data is well-described. This simple correlation may be used in order to perform quick estimation of the amount of OPF required to achieve specific biodegradation rates.
format	Thesis
qualification_level	Master's degree
author	Sukor, Mohd Zaki
author_facet	Sukor, Mohd Zaki
author_sort	Sukor, Mohd Zaki
title	Biodegradation of naphthalene in a soil medium using Pleurotus ostreatus with the addition of biomass as a nutrient / Mohd Zaki Sukor
title_short	Biodegradation of naphthalene in a soil medium using Pleurotus ostreatus with the addition of biomass as a nutrient / Mohd Zaki Sukor
title_full	Biodegradation of naphthalene in a soil medium using Pleurotus ostreatus with the addition of biomass as a nutrient / Mohd Zaki Sukor
title_fullStr	Biodegradation of naphthalene in a soil medium using Pleurotus ostreatus with the addition of biomass as a nutrient / Mohd Zaki Sukor
title_full_unstemmed	Biodegradation of naphthalene in a soil medium using Pleurotus ostreatus with the addition of biomass as a nutrient / Mohd Zaki Sukor
title_sort	biodegradation of naphthalene in a soil medium using pleurotus ostreatus with the addition of biomass as a nutrient / mohd zaki sukor
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Art and Design
publishDate	2012
url	https://ir.uitm.edu.my/id/eprint/54140/1/54140.pdf
_version_	1783734886212829184

Biodegradation of naphthalene in a soil medium using Pleurotus ostreatus with the addition of biomass as a nutrient / Mohd Zaki Sukor

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