Biological screening and molecular mechanism studies of synthesized stilbenes against human chronic myeloid leukemic K562 cells / Haslan Roslie

Stilbenes such as resveratrol, pterostilbene and picetannol were known to exhibit wide range biological activities including anticancer and anti-leukemic properties. In this study, a series of stilbene derivatives were synthesized incorporating acetoxy-, benzyloxy-, carboxy-, chloro-, hydroxy- and m...

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Main Author:	Roslie, Haslan
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Specialties of internal medicine Diseases of the blood and blood-forming organs
Online Access:	https://ir.uitm.edu.my/id/eprint/54157/1/54157.pdf
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spelling	my-uitm-ir.541572022-01-04T02:37:03Z Biological screening and molecular mechanism studies of synthesized stilbenes against human chronic myeloid leukemic K562 cells / Haslan Roslie 2012 Roslie, Haslan Specialties of internal medicine Diseases of the blood and blood-forming organs Stilbenes such as resveratrol, pterostilbene and picetannol were known to exhibit wide range biological activities including anticancer and anti-leukemic properties. In this study, a series of stilbene derivatives were synthesized incorporating acetoxy-, benzyloxy-, carboxy-, chloro-, hydroxy- and methoxy functional groups. The cytotoxicity of 23 stilbenes in human K562 chronic myelogenous leukemia cells were evaluated. Only four compounds were cytotoxic namely VS31, SY1/11B-25, VS30 and VS27 with IC50s of 78^iM, 38^M, 67uM and 19.5^M, respectively. By using Ferric Reducing "Antioxidant Power" (FRAP) assay, all compounds were investigated for their antioxidant activities and only compounds that possessed hydroxy 1-group (VS27, VS30 and VS31) have antioxidant activities. However, the FRAP value was much lower compared to resveratrol which possessed 3 hydroxylgroups. Genotoxicity assessment was carried out on two (2) most potent compounds. Compounds SY1/11B-25 and VS27 showed no DNA damage as assessed using Alkaline Comet assay in K562 cells which suggested that the cytotoxicity was independent of primary DNA damage. The apoptosis assessment using Acridine Orange/Propidium Iodide staining on VS27 and SY1/11B-25 were found to induce apoptosis at their IC50 concentration within 24 hours and the number of apoptotic, cells increased after 48 hours. On the other hand, flow cytometric analysis of phosphatidylserine exposure confirmed that the cells underwent apoptosis. Since VS27 was found to be more potent and active compared to SY1/11B-25, further studies were carried out only on VS27. The loss of mitochondrial membrane potential was observed on K562 treated with VS27. Importantly, a concentrationdependent activation of caspase-9 as early as 2 hours with resultant caspase-3 cleavage in VS27-induced apoptosis was observed. Taken together, these data suggest that the pro-apoptotic effects of VS27 involve the intrinsic mitochondrial pathway characterized by an early activation of caspase-9. 2012 Thesis https://ir.uitm.edu.my/id/eprint/54157/ https://ir.uitm.edu.my/id/eprint/54157/1/54157.pdf text en public masters Universiti Teknologi MARA Faculty of Pharmacy
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Specialties of internal medicine Diseases of the blood and blood-forming organs
spellingShingle	Specialties of internal medicine Diseases of the blood and blood-forming organs Roslie, Haslan Biological screening and molecular mechanism studies of synthesized stilbenes against human chronic myeloid leukemic K562 cells / Haslan Roslie
description	Stilbenes such as resveratrol, pterostilbene and picetannol were known to exhibit wide range biological activities including anticancer and anti-leukemic properties. In this study, a series of stilbene derivatives were synthesized incorporating acetoxy-, benzyloxy-, carboxy-, chloro-, hydroxy- and methoxy functional groups. The cytotoxicity of 23 stilbenes in human K562 chronic myelogenous leukemia cells were evaluated. Only four compounds were cytotoxic namely VS31, SY1/11B-25, VS30 and VS27 with IC50s of 78^iM, 38^M, 67uM and 19.5^M, respectively. By using Ferric Reducing "Antioxidant Power" (FRAP) assay, all compounds were investigated for their antioxidant activities and only compounds that possessed hydroxy 1-group (VS27, VS30 and VS31) have antioxidant activities. However, the FRAP value was much lower compared to resveratrol which possessed 3 hydroxylgroups. Genotoxicity assessment was carried out on two (2) most potent compounds. Compounds SY1/11B-25 and VS27 showed no DNA damage as assessed using Alkaline Comet assay in K562 cells which suggested that the cytotoxicity was independent of primary DNA damage. The apoptosis assessment using Acridine Orange/Propidium Iodide staining on VS27 and SY1/11B-25 were found to induce apoptosis at their IC50 concentration within 24 hours and the number of apoptotic, cells increased after 48 hours. On the other hand, flow cytometric analysis of phosphatidylserine exposure confirmed that the cells underwent apoptosis. Since VS27 was found to be more potent and active compared to SY1/11B-25, further studies were carried out only on VS27. The loss of mitochondrial membrane potential was observed on K562 treated with VS27. Importantly, a concentrationdependent activation of caspase-9 as early as 2 hours with resultant caspase-3 cleavage in VS27-induced apoptosis was observed. Taken together, these data suggest that the pro-apoptotic effects of VS27 involve the intrinsic mitochondrial pathway characterized by an early activation of caspase-9.
format	Thesis
qualification_level	Master's degree
author	Roslie, Haslan
author_facet	Roslie, Haslan
author_sort	Roslie, Haslan
title	Biological screening and molecular mechanism studies of synthesized stilbenes against human chronic myeloid leukemic K562 cells / Haslan Roslie
title_short	Biological screening and molecular mechanism studies of synthesized stilbenes against human chronic myeloid leukemic K562 cells / Haslan Roslie
title_full	Biological screening and molecular mechanism studies of synthesized stilbenes against human chronic myeloid leukemic K562 cells / Haslan Roslie
title_fullStr	Biological screening and molecular mechanism studies of synthesized stilbenes against human chronic myeloid leukemic K562 cells / Haslan Roslie
title_full_unstemmed	Biological screening and molecular mechanism studies of synthesized stilbenes against human chronic myeloid leukemic K562 cells / Haslan Roslie
title_sort	biological screening and molecular mechanism studies of synthesized stilbenes against human chronic myeloid leukemic k562 cells / haslan roslie
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Pharmacy
publishDate	2012
url	https://ir.uitm.edu.my/id/eprint/54157/1/54157.pdf
_version_	1783734886480216064

Biological screening and molecular mechanism studies of synthesized stilbenes against human chronic myeloid leukemic K562 cells / Haslan Roslie

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