In vitro and in vivo characterization of PLGA nanoparticles loaded with ibuprofen a poorly water soluble drug / Noreen Ang Azlan

Nanoparticles both pharmaceutical and biomedical have many advantages such as enhancing drug bioavailability and the ability to cross barriers after oral and parentral administration by improving the solubility of poorly water soluble drugs. However, physical properties of nanoparticles, such as siz...

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Main Author:	Azlan, Noreen Ang
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Pharmaceutical dosage forms Pharmaceutical chemistry
Online Access:	https://ir.uitm.edu.my/id/eprint/54170/1/54170.pdf
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spelling	my-uitm-ir.541702022-01-04T08:58:17Z In vitro and in vivo characterization of PLGA nanoparticles loaded with ibuprofen a poorly water soluble drug / Noreen Ang Azlan 2012 Azlan, Noreen Ang Pharmaceutical dosage forms Pharmaceutical chemistry Nanoparticles both pharmaceutical and biomedical have many advantages such as enhancing drug bioavailability and the ability to cross barriers after oral and parentral administration by improving the solubility of poorly water soluble drugs. However, physical properties of nanoparticles, such as size and morphological characteristics are critical for the drug release in the body, hence the importance of understanding these parameters. Poly (lactic-co-glycolic acid) (PLGA) has been studied extensively as a polymeric starting material for the fabrication of biodegradable and biocompatible nanoparticles. The pharmaceutical parameters of nanoparticles preparation have been studied using a model Class II drug, ibuprofen. The solvent evaporation technique using oil-in water (O/W) is commonly adopted for the preparation of polymeric nanoparticles. This method involves mixing an aqueous phase containing distilled water and stabilizing agent, polyvinyl alcohol (PVA) and an organic phase including the polymer and an organic solvent. The effect of preparation parameters on the particle size, size distribution, zeta potential and morphology of PLGA nanoparticles was studied. Amongst the many parameters only four critical parameters namely, homogenizing speed, homogenizing time, stabilizer concentration and temperature were investigated. The optimum parameters identified were then adopted for the preparation of ibuprofen loaded-PLGA nanoparticles. The mean particle size, polydispersity index (PDI) and zeta potential of blank PLGA nanoparticles were determined and found to be 679 nm, 0.101 and -34.5 mV, respectively. Loading the drug into the PLGA nanoparticles resulted in an increase in the, mean size, PDI and zeta potential of the nanoparticles. The surface morphology of nanoparticles was investigated by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM). The thermal behavior, crystalinity and chemical interaction of ibuprofen loaded-PLGA nanoparticles were investigated by differential scanning calorimetry (DSC), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), respectively. Three different formulations of ibuprofen loaded-PLGA nanoparticles were prepared to study the drug release behavior. The drug content and encapsulation efficiency (EE) of the three formulations were determined using HPLC and UV. The results showed an initial rapid release (burst effect) followed by a slower drug release for all formulations at different conditions. Bioavailability studies were conducted following the 2 week crossover design to evaluate the drug concentration in blood serum of rats. The following pharmacokinetic parameters (AUC, Tmax and Cmax) were calculated using PK addins of Microsoft Excel. Statistical analysis was conducted using a two-way ANOVA followed by a one-way ANOVA. Results revealed that the loaded nanoparticles showed significantly (p<0.05) higher AUC0-1 and Cmax compared with the control. In conclusion, the PLGA nanoparticles have the potential to improve the bioavailability Class II drugs and as such are likely to reduce the dose needed and minimize possible side effects. 2012 Thesis https://ir.uitm.edu.my/id/eprint/54170/ https://ir.uitm.edu.my/id/eprint/54170/1/54170.pdf text en public masters Universiti Teknologi MARA Faculty of Pharmacy
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Pharmaceutical dosage forms Pharmaceutical chemistry
spellingShingle	Pharmaceutical dosage forms Pharmaceutical chemistry Azlan, Noreen Ang In vitro and in vivo characterization of PLGA nanoparticles loaded with ibuprofen a poorly water soluble drug / Noreen Ang Azlan
description	Nanoparticles both pharmaceutical and biomedical have many advantages such as enhancing drug bioavailability and the ability to cross barriers after oral and parentral administration by improving the solubility of poorly water soluble drugs. However, physical properties of nanoparticles, such as size and morphological characteristics are critical for the drug release in the body, hence the importance of understanding these parameters. Poly (lactic-co-glycolic acid) (PLGA) has been studied extensively as a polymeric starting material for the fabrication of biodegradable and biocompatible nanoparticles. The pharmaceutical parameters of nanoparticles preparation have been studied using a model Class II drug, ibuprofen. The solvent evaporation technique using oil-in water (O/W) is commonly adopted for the preparation of polymeric nanoparticles. This method involves mixing an aqueous phase containing distilled water and stabilizing agent, polyvinyl alcohol (PVA) and an organic phase including the polymer and an organic solvent. The effect of preparation parameters on the particle size, size distribution, zeta potential and morphology of PLGA nanoparticles was studied. Amongst the many parameters only four critical parameters namely, homogenizing speed, homogenizing time, stabilizer concentration and temperature were investigated. The optimum parameters identified were then adopted for the preparation of ibuprofen loaded-PLGA nanoparticles. The mean particle size, polydispersity index (PDI) and zeta potential of blank PLGA nanoparticles were determined and found to be 679 nm, 0.101 and -34.5 mV, respectively. Loading the drug into the PLGA nanoparticles resulted in an increase in the, mean size, PDI and zeta potential of the nanoparticles. The surface morphology of nanoparticles was investigated by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM). The thermal behavior, crystalinity and chemical interaction of ibuprofen loaded-PLGA nanoparticles were investigated by differential scanning calorimetry (DSC), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), respectively. Three different formulations of ibuprofen loaded-PLGA nanoparticles were prepared to study the drug release behavior. The drug content and encapsulation efficiency (EE) of the three formulations were determined using HPLC and UV. The results showed an initial rapid release (burst effect) followed by a slower drug release for all formulations at different conditions. Bioavailability studies were conducted following the 2 week crossover design to evaluate the drug concentration in blood serum of rats. The following pharmacokinetic parameters (AUC, Tmax and Cmax) were calculated using PK addins of Microsoft Excel. Statistical analysis was conducted using a two-way ANOVA followed by a one-way ANOVA. Results revealed that the loaded nanoparticles showed significantly (p<0.05) higher AUC0-1 and Cmax compared with the control. In conclusion, the PLGA nanoparticles have the potential to improve the bioavailability Class II drugs and as such are likely to reduce the dose needed and minimize possible side effects.
format	Thesis
qualification_level	Master's degree
author	Azlan, Noreen Ang
author_facet	Azlan, Noreen Ang
author_sort	Azlan, Noreen Ang
title	In vitro and in vivo characterization of PLGA nanoparticles loaded with ibuprofen a poorly water soluble drug / Noreen Ang Azlan
title_short	In vitro and in vivo characterization of PLGA nanoparticles loaded with ibuprofen a poorly water soluble drug / Noreen Ang Azlan
title_full	In vitro and in vivo characterization of PLGA nanoparticles loaded with ibuprofen a poorly water soluble drug / Noreen Ang Azlan
title_fullStr	In vitro and in vivo characterization of PLGA nanoparticles loaded with ibuprofen a poorly water soluble drug / Noreen Ang Azlan
title_full_unstemmed	In vitro and in vivo characterization of PLGA nanoparticles loaded with ibuprofen a poorly water soluble drug / Noreen Ang Azlan
title_sort	in vitro and in vivo characterization of plga nanoparticles loaded with ibuprofen a poorly water soluble drug / noreen ang azlan
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Pharmacy
publishDate	2012
url	https://ir.uitm.edu.my/id/eprint/54170/1/54170.pdf
_version_	1783734888256503808

In vitro and in vivo characterization of PLGA nanoparticles loaded with ibuprofen a poorly water soluble drug / Noreen Ang Azlan

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