Polycyclic aromatic hydrocarbons biodegradation using isolated strains under indigenous condition / Norzila Othman

The treatment and disposal of domestic sludge is an expensive and environmentally sensitive problem. It is also a growing problem since sludge production will continue to increase as new wastewater treatment plants are built due to population increase. The large volume of domestic sludge produced ha...

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Main Author:	Othman, Norzila
Format:	Thesis
Language:	English
Published:	2010
Subjects:	Environmental effects of industries and plants
Online Access:	https://ir.uitm.edu.my/id/eprint/5468/1/TP_NORZILA%20OTHMAN%20EC%2010_5%201.pdf
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id	my-uitm-ir.5468
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spelling	my-uitm-ir.54682022-12-21T03:22:28Z Polycyclic aromatic hydrocarbons biodegradation using isolated strains under indigenous condition / Norzila Othman 2010 Othman, Norzila Environmental effects of industries and plants Special types of environment. Including soil pollution, air pollution, noise pollution The treatment and disposal of domestic sludge is an expensive and environmentally sensitive problem. It is also a growing problem since sludge production will continue to increase as new wastewater treatment plants are built due to population increase. The large volume of domestic sludge produced had made it difficult for many countries including Malaysia to assure complete treatment of the sludge before discharging to the receiving environment. Domestic sludge contains diverse range of pollutants such as pathogen, inorganic and organic compounds. These pollutants are toxic, mutagenic or carcinogenic and may threaten human health. Improper disposal and handling of sludge may pose serious impact to the environment especially on soil and water cycles. Previous studies on Malaysian domestic sludge only reported on bulk parameters and heavy metals. Thus, no study reported on organic micro pollutants, namely, polycylic aromatic hydrocarbons (PAHs). Their recalcitrance and persistence make them problematic environmental contaminants. Microbial degradation is considered to be the primary mechanism of PAHs removal from the environment. Much has been reported on biodegradation of PAHs in several countries but there is a lack of information quantitative on this subject in Malaysia. This study is carried out to understand the nature of domestic sludge and to provide a better understanding on the biodegradation processes of PAHs. The methodology of this study comprised field activities, laboratory work and mathematical modelling. Field activities involved sampling of domestic sludge from Kolej Mawar, Universiti Teknologi MARA, Shah Alam, Selangor. Laboratory activities include seven phases of experimental works. First phase is characterization study of domestic sludge based on bulk parameters, heavy metals and PAHs. Second phase is enrichment and purification of bacteria isolated from domestic sludge using single PAHs and mixed PAHs as growth substrate. This was followed by identification of bacteria using BIOLOG system. The fourth phase focussed on turbidity test to monitor growth rate of the isolated bacteria. Preliminary degradation study involves optimization of the process at different substrate concentration, bacteria concentration, pH and temperature. The optimum conditions established from optimization study were used in degradation study. In biodegradation study, two experimental conditions were performed. These conditions include using bacteria isolated from single PAHs as substrate and bacteria isolated from mixed PAHs. Protein and pH tests were done during degradation study. 2010 Thesis https://ir.uitm.edu.my/id/eprint/5468/ https://ir.uitm.edu.my/id/eprint/5468/1/TP_NORZILA%20OTHMAN%20EC%2010_5%201.pdf text en public phd doctoral Universiti Teknologi MARA Faculty of Civil Engineering
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Environmental effects of industries and plants Environmental effects of industries and plants
spellingShingle	Environmental effects of industries and plants Environmental effects of industries and plants Othman, Norzila Polycyclic aromatic hydrocarbons biodegradation using isolated strains under indigenous condition / Norzila Othman
description	The treatment and disposal of domestic sludge is an expensive and environmentally sensitive problem. It is also a growing problem since sludge production will continue to increase as new wastewater treatment plants are built due to population increase. The large volume of domestic sludge produced had made it difficult for many countries including Malaysia to assure complete treatment of the sludge before discharging to the receiving environment. Domestic sludge contains diverse range of pollutants such as pathogen, inorganic and organic compounds. These pollutants are toxic, mutagenic or carcinogenic and may threaten human health. Improper disposal and handling of sludge may pose serious impact to the environment especially on soil and water cycles. Previous studies on Malaysian domestic sludge only reported on bulk parameters and heavy metals. Thus, no study reported on organic micro pollutants, namely, polycylic aromatic hydrocarbons (PAHs). Their recalcitrance and persistence make them problematic environmental contaminants. Microbial degradation is considered to be the primary mechanism of PAHs removal from the environment. Much has been reported on biodegradation of PAHs in several countries but there is a lack of information quantitative on this subject in Malaysia. This study is carried out to understand the nature of domestic sludge and to provide a better understanding on the biodegradation processes of PAHs. The methodology of this study comprised field activities, laboratory work and mathematical modelling. Field activities involved sampling of domestic sludge from Kolej Mawar, Universiti Teknologi MARA, Shah Alam, Selangor. Laboratory activities include seven phases of experimental works. First phase is characterization study of domestic sludge based on bulk parameters, heavy metals and PAHs. Second phase is enrichment and purification of bacteria isolated from domestic sludge using single PAHs and mixed PAHs as growth substrate. This was followed by identification of bacteria using BIOLOG system. The fourth phase focussed on turbidity test to monitor growth rate of the isolated bacteria. Preliminary degradation study involves optimization of the process at different substrate concentration, bacteria concentration, pH and temperature. The optimum conditions established from optimization study were used in degradation study. In biodegradation study, two experimental conditions were performed. These conditions include using bacteria isolated from single PAHs as substrate and bacteria isolated from mixed PAHs. Protein and pH tests were done during degradation study.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Othman, Norzila
author_facet	Othman, Norzila
author_sort	Othman, Norzila
title	Polycyclic aromatic hydrocarbons biodegradation using isolated strains under indigenous condition / Norzila Othman
title_short	Polycyclic aromatic hydrocarbons biodegradation using isolated strains under indigenous condition / Norzila Othman
title_full	Polycyclic aromatic hydrocarbons biodegradation using isolated strains under indigenous condition / Norzila Othman
title_fullStr	Polycyclic aromatic hydrocarbons biodegradation using isolated strains under indigenous condition / Norzila Othman
title_full_unstemmed	Polycyclic aromatic hydrocarbons biodegradation using isolated strains under indigenous condition / Norzila Othman
title_sort	polycyclic aromatic hydrocarbons biodegradation using isolated strains under indigenous condition / norzila othman
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Civil Engineering
publishDate	2010
url	https://ir.uitm.edu.my/id/eprint/5468/1/TP_NORZILA%20OTHMAN%20EC%2010_5%201.pdf
_version_	1783733125089591296

Polycyclic aromatic hydrocarbons biodegradation using isolated strains under indigenous condition / Norzila Othman

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