pH-sensitive nanoparticles as carriers for oral delivery of a model peptidomimetic drug / Rosa Elizabeth Valente Pereira

Peptide-related drugs are usually unstable in the harsh gastrointestinal environment with poor absorption throughout the gut. The potential of anionic pH-sensitive nanoparticles to act as novel drug carriers for oral administration of such compounds was investigated using a peptidomimetic drug, cefo...

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Main Author:	Valente Pereira, Rosa Elizabeth
Format:	Thesis
Language:	English
Published:	2008
Subjects:	Drugs and their actions Pharmaceutical dosage forms
Online Access:	https://ir.uitm.edu.my/id/eprint/5480/2/5480.pdf
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id	my-uitm-ir.5480
record_format	uketd_dc
spelling	my-uitm-ir.54802022-12-06T00:34:08Z pH-sensitive nanoparticles as carriers for oral delivery of a model peptidomimetic drug / Rosa Elizabeth Valente Pereira 2008 Valente Pereira, Rosa Elizabeth Drugs and their actions Pharmaceutical dosage forms Peptide-related drugs are usually unstable in the harsh gastrointestinal environment with poor absorption throughout the gut. The potential of anionic pH-sensitive nanoparticles to act as novel drug carriers for oral administration of such compounds was investigated using a peptidomimetic drug, cefotaxime sodium. Drug loaded nanoparticles were prepared using a pH-controlled nanoprecipitation method. Three types of nanoparticles were prepared using different grades of Eudragit polymer, namely LI00, LI00-55 and SI00. The drug-loaded nanoparticles have a size of about 100 nm, low polydispersity, encapsulation efficiencies of more than 50%, drug entrapment and drug content between 4-5%. Bioavailability studies were initially conducted in rats according to a crossover design. However, a carry-over effect was observed with visible reduction of cefotaxime absorption following the first exposure of the rats to the drug-loaded nanoparticles. This phenomenon was confirmed with repeated administration of the same drug-loaded nanoparticles to the same rats (after one week wash-out period). Thus, in subsequent studies, a parallel group design was used instead. The extent of cefotaxime bioavailability was found to increase in the following order with the different polymeric nanoparticles: S100>L100-55>L100. Bioavailability of the nanoparticles from isolated segments of rat intestines was further investigated to determine if they were preferentially absorbed from specific regions. Absorption was found to occur in the entire small intestine. However, the duodenum, jejunum and jejunum-ileum areas were observed to be the optimal sites for absorption of the L100, L100-55, and S100 nanoparticles respectively. Finally, the drug-loaded nanoparticles were also observed to enhance the lymphatic transport of the contained drug. The ratio of the cefotaxime concentration in the lymph over that of the plasma obtained with administration of the nanoparticles was consistently higher than that obtained with administration of an aqueous solution of the drug, suggesting that nanoparticles could promote the lymphatic transport of the drug. 2008 Thesis https://ir.uitm.edu.my/id/eprint/5480/ https://ir.uitm.edu.my/id/eprint/5480/2/5480.pdf text en public phd doctoral Universiti Teknologi MARA (UiTM) Faculty of Pharmacy Abdul Majeed, Abu Bakar
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Abdul Majeed, Abu Bakar
topic	Drugs and their actions Pharmaceutical dosage forms
spellingShingle	Drugs and their actions Pharmaceutical dosage forms Valente Pereira, Rosa Elizabeth pH-sensitive nanoparticles as carriers for oral delivery of a model peptidomimetic drug / Rosa Elizabeth Valente Pereira
description	Peptide-related drugs are usually unstable in the harsh gastrointestinal environment with poor absorption throughout the gut. The potential of anionic pH-sensitive nanoparticles to act as novel drug carriers for oral administration of such compounds was investigated using a peptidomimetic drug, cefotaxime sodium. Drug loaded nanoparticles were prepared using a pH-controlled nanoprecipitation method. Three types of nanoparticles were prepared using different grades of Eudragit polymer, namely LI00, LI00-55 and SI00. The drug-loaded nanoparticles have a size of about 100 nm, low polydispersity, encapsulation efficiencies of more than 50%, drug entrapment and drug content between 4-5%. Bioavailability studies were initially conducted in rats according to a crossover design. However, a carry-over effect was observed with visible reduction of cefotaxime absorption following the first exposure of the rats to the drug-loaded nanoparticles. This phenomenon was confirmed with repeated administration of the same drug-loaded nanoparticles to the same rats (after one week wash-out period). Thus, in subsequent studies, a parallel group design was used instead. The extent of cefotaxime bioavailability was found to increase in the following order with the different polymeric nanoparticles: S100>L100-55>L100. Bioavailability of the nanoparticles from isolated segments of rat intestines was further investigated to determine if they were preferentially absorbed from specific regions. Absorption was found to occur in the entire small intestine. However, the duodenum, jejunum and jejunum-ileum areas were observed to be the optimal sites for absorption of the L100, L100-55, and S100 nanoparticles respectively. Finally, the drug-loaded nanoparticles were also observed to enhance the lymphatic transport of the contained drug. The ratio of the cefotaxime concentration in the lymph over that of the plasma obtained with administration of the nanoparticles was consistently higher than that obtained with administration of an aqueous solution of the drug, suggesting that nanoparticles could promote the lymphatic transport of the drug.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Valente Pereira, Rosa Elizabeth
author_facet	Valente Pereira, Rosa Elizabeth
author_sort	Valente Pereira, Rosa Elizabeth
title	pH-sensitive nanoparticles as carriers for oral delivery of a model peptidomimetic drug / Rosa Elizabeth Valente Pereira
title_short	pH-sensitive nanoparticles as carriers for oral delivery of a model peptidomimetic drug / Rosa Elizabeth Valente Pereira
title_full	pH-sensitive nanoparticles as carriers for oral delivery of a model peptidomimetic drug / Rosa Elizabeth Valente Pereira
title_fullStr	pH-sensitive nanoparticles as carriers for oral delivery of a model peptidomimetic drug / Rosa Elizabeth Valente Pereira
title_full_unstemmed	pH-sensitive nanoparticles as carriers for oral delivery of a model peptidomimetic drug / Rosa Elizabeth Valente Pereira
title_sort	ph-sensitive nanoparticles as carriers for oral delivery of a model peptidomimetic drug / rosa elizabeth valente pereira
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Pharmacy
publishDate	2008
url	https://ir.uitm.edu.my/id/eprint/5480/2/5480.pdf
_version_	1783733126228344832

pH-sensitive nanoparticles as carriers for oral delivery of a model peptidomimetic drug / Rosa Elizabeth Valente Pereira

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