Inhibitory effect of 5-AZA-DC and expression of MIR-129-3P in ORL-48t / Nur Rawaidah Mohd Shobri

Oral cancer is recorded as the sixth highest malignancy globally. In Malaysia, oral cancer deaths have been reached 1,060 or 0.83% of total deaths in 2018. Current cases reported that oral cancer can be treated when detected at an early stage of the disease. However, so many cases are still detected...

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Main Author:	Mohd Shobri, Nur Rawaidah
Format:	Thesis
Language:	English
Published:	2021
Subjects:	Cancer
Online Access:	https://ir.uitm.edu.my/id/eprint/59816/1/59816.pdf
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id	my-uitm-ir.59816
record_format	uketd_dc
spelling	my-uitm-ir.598162022-05-18T05:00:11Z Inhibitory effect of 5-AZA-DC and expression of MIR-129-3P in ORL-48t / Nur Rawaidah Mohd Shobri 2021-01 Mohd Shobri, Nur Rawaidah Cancer Oral cancer is recorded as the sixth highest malignancy globally. In Malaysia, oral cancer deaths have been reached 1,060 or 0.83% of total deaths in 2018. Current cases reported that oral cancer can be treated when detected at an early stage of the disease. However, so many cases are still detected at a late stage of disease, thus increasing the mortality rate of the patients. MicroRNAs (miRNAs) is a group of small non-coding RNAs, which plays a role in post-transcriptionally regulating gene expression, and miR-129-3p was reported affecting the cell proliferation, apoptosis, and even chemotherapy resistance in different types of cancer. Research has shown that deregulation of miRNAs in almost every type of human cancer, and the signature of miRNA expression in tumour can be potentially applied in cancer detection and prognosis. Epigenetic drugs, 5-aza-2'-deoxycytidine (5-aza-dC) function as a methylation inhibitor, also is implemented in treating cancer which possibly reverses aberrant gene expression profiles associated with different diseases. The aim of this study is to measure the inhibitory effect 5-aza-dC towards proliferation of the oral cancer cell line, ORL-48T and normal human gingival fibroblast, hGF using an in vitro model. In addition, the evaluation of miRNA-129-3p expression of treated ORL-48T and hGF with 5-aza-dC using quantitative Real-Time Polymerase Chain Reaction (RT-PCR) was also carried out in this study. Inhibitory effects of 5-aza-dC on ORL-48T and hGF proliferation was studied using 3-4,5-dimethylthiazol-2-yl-2,5 -diphenyltetrazolium bromide (MTT)-based assay. Statistical analysis revealed that treatment with 40 μM of 5-aza-dC for 72 h, and 20 μM of 5-aza-dC for 144 h had significantly decreased the viability of oral cancer cells (p=0.03). The expression of miR-129-3p was also found to be significant between the treated and untreated ORL-48T (p=0.02). However, there was no significant difference between treated and untreated hGF. Overall studies indicate that miR-129-3p was upregulated in an oral cancer cell which showed a signal of oncogenesis and potential target to detect oral cancer. These findings also suggested the inhibitive effect of 5-aza-dC towards the proliferation of ORL-48T cells. Therefore, the findings from this study would be useful for detection, prognostic, diagnostic or therapeutic targets of oral cancer and improve the survival rate of oral cancer patients in the future. Moreover, it could help in the development of effective cancer treatment by applying miR-129-3p as potential biomarker in detecting oral cancer. 2021-01 Thesis https://ir.uitm.edu.my/id/eprint/59816/ https://ir.uitm.edu.my/id/eprint/59816/1/59816.pdf text en public masters Universiti Teknologi MARA Faculty of Dentistry
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Cancer
spellingShingle	Cancer Mohd Shobri, Nur Rawaidah Inhibitory effect of 5-AZA-DC and expression of MIR-129-3P in ORL-48t / Nur Rawaidah Mohd Shobri
description	Oral cancer is recorded as the sixth highest malignancy globally. In Malaysia, oral cancer deaths have been reached 1,060 or 0.83% of total deaths in 2018. Current cases reported that oral cancer can be treated when detected at an early stage of the disease. However, so many cases are still detected at a late stage of disease, thus increasing the mortality rate of the patients. MicroRNAs (miRNAs) is a group of small non-coding RNAs, which plays a role in post-transcriptionally regulating gene expression, and miR-129-3p was reported affecting the cell proliferation, apoptosis, and even chemotherapy resistance in different types of cancer. Research has shown that deregulation of miRNAs in almost every type of human cancer, and the signature of miRNA expression in tumour can be potentially applied in cancer detection and prognosis. Epigenetic drugs, 5-aza-2'-deoxycytidine (5-aza-dC) function as a methylation inhibitor, also is implemented in treating cancer which possibly reverses aberrant gene expression profiles associated with different diseases. The aim of this study is to measure the inhibitory effect 5-aza-dC towards proliferation of the oral cancer cell line, ORL-48T and normal human gingival fibroblast, hGF using an in vitro model. In addition, the evaluation of miRNA-129-3p expression of treated ORL-48T and hGF with 5-aza-dC using quantitative Real-Time Polymerase Chain Reaction (RT-PCR) was also carried out in this study. Inhibitory effects of 5-aza-dC on ORL-48T and hGF proliferation was studied using 3-4,5-dimethylthiazol-2-yl-2,5 -diphenyltetrazolium bromide (MTT)-based assay. Statistical analysis revealed that treatment with 40 μM of 5-aza-dC for 72 h, and 20 μM of 5-aza-dC for 144 h had significantly decreased the viability of oral cancer cells (p=0.03). The expression of miR-129-3p was also found to be significant between the treated and untreated ORL-48T (p=0.02). However, there was no significant difference between treated and untreated hGF. Overall studies indicate that miR-129-3p was upregulated in an oral cancer cell which showed a signal of oncogenesis and potential target to detect oral cancer. These findings also suggested the inhibitive effect of 5-aza-dC towards the proliferation of ORL-48T cells. Therefore, the findings from this study would be useful for detection, prognostic, diagnostic or therapeutic targets of oral cancer and improve the survival rate of oral cancer patients in the future. Moreover, it could help in the development of effective cancer treatment by applying miR-129-3p as potential biomarker in detecting oral cancer.
format	Thesis
qualification_level	Master's degree
author	Mohd Shobri, Nur Rawaidah
author_facet	Mohd Shobri, Nur Rawaidah
author_sort	Mohd Shobri, Nur Rawaidah
title	Inhibitory effect of 5-AZA-DC and expression of MIR-129-3P in ORL-48t / Nur Rawaidah Mohd Shobri
title_short	Inhibitory effect of 5-AZA-DC and expression of MIR-129-3P in ORL-48t / Nur Rawaidah Mohd Shobri
title_full	Inhibitory effect of 5-AZA-DC and expression of MIR-129-3P in ORL-48t / Nur Rawaidah Mohd Shobri
title_fullStr	Inhibitory effect of 5-AZA-DC and expression of MIR-129-3P in ORL-48t / Nur Rawaidah Mohd Shobri
title_full_unstemmed	Inhibitory effect of 5-AZA-DC and expression of MIR-129-3P in ORL-48t / Nur Rawaidah Mohd Shobri
title_sort	inhibitory effect of 5-aza-dc and expression of mir-129-3p in orl-48t / nur rawaidah mohd shobri
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Dentistry
publishDate	2021
url	https://ir.uitm.edu.my/id/eprint/59816/1/59816.pdf
_version_	1783735058640666624

Inhibitory effect of 5-AZA-DC and expression of MIR-129-3P in ORL-48t / Nur Rawaidah Mohd Shobri

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