The stability of DNA in chloride-malonic acid deep eutectic solvent at different concentration / Nur Fikriyah Ariffin
DNA is one of the most essential macromolecules in cells, holding the genetic information needed for living beings to grow and function. Finding a medium in which DNA is both soluble and stable over a long length of time is a tough task. As a result, deep eutectic solvents (DES) were proposed as a n...
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| Format: | Thesis |
| Language: | English |
| Published: |
2022
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| Subjects: | |
| Online Access: | https://ir.uitm.edu.my/id/eprint/59907/1/59907.pdf |
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| Summary: | DNA is one of the most essential macromolecules in cells, holding the genetic information needed for living beings to grow and function. Finding a medium in which DNA is both soluble and stable over a long length of time is a tough task. As a result, deep eutectic solvents (DES) were proposed as a novel solvent for this study to replace water or aqueous solutions. The purpose of this research is to identify effect of different concentration of DES on DNA as storage medium using a UV- Visible Spectrophotometer. DES was prepared by heating malonic acid and choline chloride at ratio 1:1 and 80 °C until a clear liquid emerged. Following that, a little amount of calf thymus DNA was dissolved and homogeneously mixed in deionised water in an ice bath (4 °C) for 24 hours. For UV-Vis, 100 μm DNA was produced and combined with various concentrations of hydrated DES: 25%, 50%, and 75%. The pure DNA concentration was 0.0875846 M. The purity of DNA is 1.89 using the A260/A280 calculation. 25% displays the closest peak with control, a concentration of 25% was determined to preserve the stability of DNA. FTIR analysis revealed a few functional groups in pure DNA, pure DES solution and DNA in DES solution. |
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