Differential protein profile in bone remodelling induced by denture wear / Umi Hairun Anis Ismail

Removable partial and complete dentures are routinely used to replace missing teeth in partially and completely edentulous patients respectively. However, as these dentures obtain their primary support from the mucosa and the underlying bone to function effectively, denture wearing has been assoc...

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Main Author:	Ismail, Umi Hairun Anis
Format:	Thesis
Language:	English
Published:	2020
Subjects:	RK Dentistry
Online Access:	https://ir.uitm.edu.my/id/eprint/60005/1/60005.pdf
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spelling	my-uitm-ir.600052022-05-20T10:39:58Z Differential protein profile in bone remodelling induced by denture wear / Umi Hairun Anis Ismail 2020-07 Ismail, Umi Hairun Anis RK Dentistry Removable partial and complete dentures are routinely used to replace missing teeth in partially and completely edentulous patients respectively. However, as these dentures obtain their primary support from the mucosa and the underlying bone to function effectively, denture wearing has been associated with an increased rate of residual ridge resorption (RRR). The continuous and intermittent mechanical pressure exerted by these dentures on the underlying soft tissue mucosa has been shown to induce inflammation and led to the phenomena of alveolar bone resorption and triggered the secretion of various proteins and enzymes into the saliva. This study aimed to identify the differential protein profile associated with bone remodelling induced by denture wear. These differentially expressed proteins could potentially serve as biomarkers for bone remodelling in denture wearing patients. This research was based on the data obtained from the saliva samples of 6 Removable Partial Denture and 14 Complete Denture patients. Unstimulated whole saliva was collected twice, once before the issuance of denture (T0) and another after 30 days of denture insertion (T1). Salivary proteins were resolved using two-dimensional gel electrophoresis (2DE) over a pH range of 3-10, and the resulting proteome profiles were compared using Image J software. The differentially expressed proteins with significant p-value ≤ 0.05 were further identified by LC/MS Q-TOF. Based on the functional annotation analysis, 8 of the identified proteins were found to have functions that are potentially associated with the mechanism of inflammation and bone remodelling process. The proteins were Serine/threonine-protein kinase 38 (p=0.003), G-protein coupled receptor (p=0.003), Interleukin-31 receptor subunit alpha (p=0.012), PTB-containing, cubilin and LRP1- interacting protein (p=0.005), Fibroblast growth factor 11 (p=0.003), BMP and activin membrane-bound inhibitor homolog (p=0.002) and Granzyme B splice variant 2 (p=0.001). In conclusion, differential protein profile associated with bone remodelling induced by denture wear have been identified and these proteins could potentially serve as biomarkers for bone remodelling in denture wearing patients. 2020-07 Thesis https://ir.uitm.edu.my/id/eprint/60005/ https://ir.uitm.edu.my/id/eprint/60005/1/60005.pdf text en public masters Universiti Teknologi MARA Faculty of Dentistry Ahmad, Rohana (Associate Professor Dr.)
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Ahmad, Rohana (Associate Professor Dr.)
topic	RK Dentistry
spellingShingle	RK Dentistry Ismail, Umi Hairun Anis Differential protein profile in bone remodelling induced by denture wear / Umi Hairun Anis Ismail
description	Removable partial and complete dentures are routinely used to replace missing teeth in partially and completely edentulous patients respectively. However, as these dentures obtain their primary support from the mucosa and the underlying bone to function effectively, denture wearing has been associated with an increased rate of residual ridge resorption (RRR). The continuous and intermittent mechanical pressure exerted by these dentures on the underlying soft tissue mucosa has been shown to induce inflammation and led to the phenomena of alveolar bone resorption and triggered the secretion of various proteins and enzymes into the saliva. This study aimed to identify the differential protein profile associated with bone remodelling induced by denture wear. These differentially expressed proteins could potentially serve as biomarkers for bone remodelling in denture wearing patients. This research was based on the data obtained from the saliva samples of 6 Removable Partial Denture and 14 Complete Denture patients. Unstimulated whole saliva was collected twice, once before the issuance of denture (T0) and another after 30 days of denture insertion (T1). Salivary proteins were resolved using two-dimensional gel electrophoresis (2DE) over a pH range of 3-10, and the resulting proteome profiles were compared using Image J software. The differentially expressed proteins with significant p-value ≤ 0.05 were further identified by LC/MS Q-TOF. Based on the functional annotation analysis, 8 of the identified proteins were found to have functions that are potentially associated with the mechanism of inflammation and bone remodelling process. The proteins were Serine/threonine-protein kinase 38 (p=0.003), G-protein coupled receptor (p=0.003), Interleukin-31 receptor subunit alpha (p=0.012), PTB-containing, cubilin and LRP1- interacting protein (p=0.005), Fibroblast growth factor 11 (p=0.003), BMP and activin membrane-bound inhibitor homolog (p=0.002) and Granzyme B splice variant 2 (p=0.001). In conclusion, differential protein profile associated with bone remodelling induced by denture wear have been identified and these proteins could potentially serve as biomarkers for bone remodelling in denture wearing patients.
format	Thesis
qualification_level	Master's degree
author	Ismail, Umi Hairun Anis
author_facet	Ismail, Umi Hairun Anis
author_sort	Ismail, Umi Hairun Anis
title	Differential protein profile in bone remodelling induced by denture wear / Umi Hairun Anis Ismail
title_short	Differential protein profile in bone remodelling induced by denture wear / Umi Hairun Anis Ismail
title_full	Differential protein profile in bone remodelling induced by denture wear / Umi Hairun Anis Ismail
title_fullStr	Differential protein profile in bone remodelling induced by denture wear / Umi Hairun Anis Ismail
title_full_unstemmed	Differential protein profile in bone remodelling induced by denture wear / Umi Hairun Anis Ismail
title_sort	differential protein profile in bone remodelling induced by denture wear / umi hairun anis ismail
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Dentistry
publishDate	2020
url	https://ir.uitm.edu.my/id/eprint/60005/1/60005.pdf
_version_	1783735069267984384

Differential protein profile in bone remodelling induced by denture wear / Umi Hairun Anis Ismail

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