MPN-duplex PCR detection for salmonella spp. contamination and survival in coconut milk / Nurul Ain Hasani

Salmonella spp. was the most common xause to human Salmonellosis worldwide in the recent years. Up to October 2018, a total of 83 cases of Salmonella strain outbreak in Malaysia had been identified. The epidemiologic investigation done before had showed a significant association between consumpti...

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Main Author:	Hasani, Nurul Ain
Format:	Thesis
Language:	English
Published:	2020
Subjects:	Processes operations and techniques
Online Access:	https://ir.uitm.edu.my/id/eprint/60007/1/60007.pdf
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id	my-uitm-ir.60007
record_format	uketd_dc
spelling	my-uitm-ir.600072022-05-20T10:40:46Z MPN-duplex PCR detection for salmonella spp. contamination and survival in coconut milk / Nurul Ain Hasani 2020-07 Hasani, Nurul Ain Processes, operations, and techniques Salmonella spp. was the most common xause to human Salmonellosis worldwide in the recent years. Up to October 2018, a total of 83 cases of Salmonella strain outbreak in Malaysia had been identified. The epidemiologic investigation done before had showed a significant association between consumption of local food containing coconut milk and gastrointestinal symptoms in Malaysia. The purpose of this study was to investigate the presence and susceptibility of Salmonella spp. and Salmonella Typhimurium in fresh coconut milk at retail level in Negeri Sembilan, Malaysia. A simulation of decontamination was also conducted to provide the best handling practices while storing a fresh coconut milk. A total of 120 samples, were randomly purchased from hypermarket and wet market all around Kuala Pilah, Seremban and Senawang areas at over one year period (March 2014 until October 2015). The range of the detection of Salmonella spp. in the sample were ranged from 3.0 x 104 to 2.4 x 107 MPN/ml. However, the highest microbial loads was found to be at the hypermarket and wet market in Kuala Pilah with 2.4 x 107 MPN/ml, respectively. A combination technique of most probable number and duplex polymerase chain reaction (MPN-PCR) method was used then to quantify on the prevalence and number of total Salmonella spp. in the fresh coconut milk in Negeri Sembilan, Malaysia. The prevalence of Salmonella spp. was 74.2% followed by Salmonella Typhimurium with 29.2% from all the three sampling areas respectively. Here, the highest detection of 85% was for Salmonella spp. from Seremban and Senawang wet market samples. A slightly low detection of 50% was detected of the Salmonella Typhimurium from Seremban hypermarket and wet market samples. Meanwhile, the lowest presence of 15% were found from the Senawang hypermarket and wet market with 15%. A simulation study was conducted to overcome the final problem of situation found Salmonella spp. in the fresh coconut milk. The three treatment were conducted in this simulation. It was found in domestic kitchen as much as to give a realistic quantitative load on how to reduced that Treatment 3, by adding 2 gram of NaCl2 and boiled up to 60°C for 10 minutes able to reduce the number of Salmonella spp culture after treatment to 3.0 x 104 CFU/ml. In conclusion, we found out that fresh coconut milk was easily been contaminated and thus pose a risk for consumers. Further studies on a bigger scale are recommended to understand the risks when handling and consuming a contaminated fresh coconut milk. 2020-07 Thesis https://ir.uitm.edu.my/id/eprint/60007/ https://ir.uitm.edu.my/id/eprint/60007/1/60007.pdf text en public masters Universiti Teknologi MARA Faculty of Applied Sciences Ahmad, Noorlis (Dr.)
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Ahmad, Noorlis (Dr.)
topic	Processes operations and techniques
spellingShingle	Processes operations and techniques Hasani, Nurul Ain MPN-duplex PCR detection for salmonella spp. contamination and survival in coconut milk / Nurul Ain Hasani
description	Salmonella spp. was the most common xause to human Salmonellosis worldwide in the recent years. Up to October 2018, a total of 83 cases of Salmonella strain outbreak in Malaysia had been identified. The epidemiologic investigation done before had showed a significant association between consumption of local food containing coconut milk and gastrointestinal symptoms in Malaysia. The purpose of this study was to investigate the presence and susceptibility of Salmonella spp. and Salmonella Typhimurium in fresh coconut milk at retail level in Negeri Sembilan, Malaysia. A simulation of decontamination was also conducted to provide the best handling practices while storing a fresh coconut milk. A total of 120 samples, were randomly purchased from hypermarket and wet market all around Kuala Pilah, Seremban and Senawang areas at over one year period (March 2014 until October 2015). The range of the detection of Salmonella spp. in the sample were ranged from 3.0 x 104 to 2.4 x 107 MPN/ml. However, the highest microbial loads was found to be at the hypermarket and wet market in Kuala Pilah with 2.4 x 107 MPN/ml, respectively. A combination technique of most probable number and duplex polymerase chain reaction (MPN-PCR) method was used then to quantify on the prevalence and number of total Salmonella spp. in the fresh coconut milk in Negeri Sembilan, Malaysia. The prevalence of Salmonella spp. was 74.2% followed by Salmonella Typhimurium with 29.2% from all the three sampling areas respectively. Here, the highest detection of 85% was for Salmonella spp. from Seremban and Senawang wet market samples. A slightly low detection of 50% was detected of the Salmonella Typhimurium from Seremban hypermarket and wet market samples. Meanwhile, the lowest presence of 15% were found from the Senawang hypermarket and wet market with 15%. A simulation study was conducted to overcome the final problem of situation found Salmonella spp. in the fresh coconut milk. The three treatment were conducted in this simulation. It was found in domestic kitchen as much as to give a realistic quantitative load on how to reduced that Treatment 3, by adding 2 gram of NaCl2 and boiled up to 60°C for 10 minutes able to reduce the number of Salmonella spp culture after treatment to 3.0 x 104 CFU/ml. In conclusion, we found out that fresh coconut milk was easily been contaminated and thus pose a risk for consumers. Further studies on a bigger scale are recommended to understand the risks when handling and consuming a contaminated fresh coconut milk.
format	Thesis
qualification_level	Master's degree
author	Hasani, Nurul Ain
author_facet	Hasani, Nurul Ain
author_sort	Hasani, Nurul Ain
title	MPN-duplex PCR detection for salmonella spp. contamination and survival in coconut milk / Nurul Ain Hasani
title_short	MPN-duplex PCR detection for salmonella spp. contamination and survival in coconut milk / Nurul Ain Hasani
title_full	MPN-duplex PCR detection for salmonella spp. contamination and survival in coconut milk / Nurul Ain Hasani
title_fullStr	MPN-duplex PCR detection for salmonella spp. contamination and survival in coconut milk / Nurul Ain Hasani
title_full_unstemmed	MPN-duplex PCR detection for salmonella spp. contamination and survival in coconut milk / Nurul Ain Hasani
title_sort	mpn-duplex pcr detection for salmonella spp. contamination and survival in coconut milk / nurul ain hasani
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2020
url	https://ir.uitm.edu.my/id/eprint/60007/1/60007.pdf
_version_	1783735069845749760

MPN-duplex PCR detection for salmonella spp. contamination and survival in coconut milk / Nurul Ain Hasani

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