Identification and characterization of exopolysaccharide produced by Bifidobacterium pseudocatenulatum ATCC 27919 cultivated in rice water medium against colon cancer cell / Jeganathan Tharshan Jeyakanesh

Exopolysaccharides (EPS) produced by probiotics have generated increasing attention among researchers due to its beneficial effect as anti-cancer property. However, it has been hypothesized that different cultivation medium used during probiotic cultivation resulted in different carbon profiles of e...

Full description

Saved in:

Bibliographic Details
Main Author:	Jeyakanesh, Jeganathan Tharshan
Format:	Thesis
Language:	English
Published:	2021
Subjects:	Cultures and culture media Culture technique. Cancer
Online Access:	https://ir.uitm.edu.my/id/eprint/60209/1/60209.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uitm-ir.60209
record_format	uketd_dc
spelling	my-uitm-ir.602092022-05-24T05:36:42Z Identification and characterization of exopolysaccharide produced by Bifidobacterium pseudocatenulatum ATCC 27919 cultivated in rice water medium against colon cancer cell / Jeganathan Tharshan Jeyakanesh 2021-09 Jeyakanesh, Jeganathan Tharshan Cultures and culture media. Culture technique. Cancer Exopolysaccharides (EPS) produced by probiotics have generated increasing attention among researchers due to its beneficial effect as anti-cancer property. However, it has been hypothesized that different cultivation medium used during probiotic cultivation resulted in different carbon profiles of exopolysaccharides to leading to anti-cancer activity. Thus, in this study, rice water was used to cultivate Bifidobacteria pseudocatenulatum ATCC 27919 and commercial media was used as comparative medium. The objectives of this study were 1) To determine the cytotoxicity effect against Caco-2 cell line using EPS cultivated in rice water and aloe vera medium using MTS assay. 2) To identify the monosaccharide of EPS using thin-layer chromatography (TLC) cultivated in the selected medium 3) To characterize the structure of EPS using flourier-transform infrared (FT-IR), nuclear magnetic resonance (NMR) spectroscopy techniques and CHNS elemental analysis. Gel filtration method was used for EPS purification. Cytotoxicity experiments showed the percentage of the cell viability of EPS from rice water medium (52%) remarkably close to EPS from commercial medium (MRS) (38%) after 40 hrs of treatment. CHNS analysis was carried out to calculate the composition of carbon, hydrogen, nitrogen, sulphur. NMR analysis was done by dissolving EPS in D2O and conducted the entire 1D and 2D NMR procedure at 47 °C. Based on the monomer identifications using TLC, glucose was found to be the main repeating unit in this compound. FTIR, CHNS, and NMR analysis were proved the presence of the one monomer. Based on this study, the arrangement of the glucose repeating unit glycosidic linkage could be α-(1→4) Glcp is the backbone of the repeating unit, and α-(1→6) glycosidic link makes the branch for this structure. Cell viability testing performed in both rice water and commercial media did not show a significant difference (p>0.05) due to the same repeating unit structure for both EPS samples. Hence, this study manifests that EPS from rice water have the potential to be used in clone cancer therapy. 2021-09 Thesis https://ir.uitm.edu.my/id/eprint/60209/ https://ir.uitm.edu.my/id/eprint/60209/1/60209.pdf text en public masters Universiti Teknologi MARA Faculty of Applied Sciences Abd Khalil, Khalilah (Associate Professor Dr.)
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Abd Khalil, Khalilah (Associate Professor Dr.)
topic	Cultures and culture media Culture technique. Cancer
spellingShingle	Cultures and culture media Culture technique. Cancer Jeyakanesh, Jeganathan Tharshan Identification and characterization of exopolysaccharide produced by Bifidobacterium pseudocatenulatum ATCC 27919 cultivated in rice water medium against colon cancer cell / Jeganathan Tharshan Jeyakanesh
description	Exopolysaccharides (EPS) produced by probiotics have generated increasing attention among researchers due to its beneficial effect as anti-cancer property. However, it has been hypothesized that different cultivation medium used during probiotic cultivation resulted in different carbon profiles of exopolysaccharides to leading to anti-cancer activity. Thus, in this study, rice water was used to cultivate Bifidobacteria pseudocatenulatum ATCC 27919 and commercial media was used as comparative medium. The objectives of this study were 1) To determine the cytotoxicity effect against Caco-2 cell line using EPS cultivated in rice water and aloe vera medium using MTS assay. 2) To identify the monosaccharide of EPS using thin-layer chromatography (TLC) cultivated in the selected medium 3) To characterize the structure of EPS using flourier-transform infrared (FT-IR), nuclear magnetic resonance (NMR) spectroscopy techniques and CHNS elemental analysis. Gel filtration method was used for EPS purification. Cytotoxicity experiments showed the percentage of the cell viability of EPS from rice water medium (52%) remarkably close to EPS from commercial medium (MRS) (38%) after 40 hrs of treatment. CHNS analysis was carried out to calculate the composition of carbon, hydrogen, nitrogen, sulphur. NMR analysis was done by dissolving EPS in D2O and conducted the entire 1D and 2D NMR procedure at 47 °C. Based on the monomer identifications using TLC, glucose was found to be the main repeating unit in this compound. FTIR, CHNS, and NMR analysis were proved the presence of the one monomer. Based on this study, the arrangement of the glucose repeating unit glycosidic linkage could be α-(1→4) Glcp is the backbone of the repeating unit, and α-(1→6) glycosidic link makes the branch for this structure. Cell viability testing performed in both rice water and commercial media did not show a significant difference (p>0.05) due to the same repeating unit structure for both EPS samples. Hence, this study manifests that EPS from rice water have the potential to be used in clone cancer therapy.
format	Thesis
qualification_level	Master's degree
author	Jeyakanesh, Jeganathan Tharshan
author_facet	Jeyakanesh, Jeganathan Tharshan
author_sort	Jeyakanesh, Jeganathan Tharshan
title	Identification and characterization of exopolysaccharide produced by Bifidobacterium pseudocatenulatum ATCC 27919 cultivated in rice water medium against colon cancer cell / Jeganathan Tharshan Jeyakanesh
title_short	Identification and characterization of exopolysaccharide produced by Bifidobacterium pseudocatenulatum ATCC 27919 cultivated in rice water medium against colon cancer cell / Jeganathan Tharshan Jeyakanesh
title_full	Identification and characterization of exopolysaccharide produced by Bifidobacterium pseudocatenulatum ATCC 27919 cultivated in rice water medium against colon cancer cell / Jeganathan Tharshan Jeyakanesh
title_fullStr	Identification and characterization of exopolysaccharide produced by Bifidobacterium pseudocatenulatum ATCC 27919 cultivated in rice water medium against colon cancer cell / Jeganathan Tharshan Jeyakanesh
title_full_unstemmed	Identification and characterization of exopolysaccharide produced by Bifidobacterium pseudocatenulatum ATCC 27919 cultivated in rice water medium against colon cancer cell / Jeganathan Tharshan Jeyakanesh
title_sort	identification and characterization of exopolysaccharide produced by bifidobacterium pseudocatenulatum atcc 27919 cultivated in rice water medium against colon cancer cell / jeganathan tharshan jeyakanesh
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2021
url	https://ir.uitm.edu.my/id/eprint/60209/1/60209.pdf
_version_	1783735099227897856

Identification and characterization of exopolysaccharide produced by Bifidobacterium pseudocatenulatum ATCC 27919 cultivated in rice water medium against colon cancer cell / Jeganathan Tharshan Jeyakanesh

Similar Items