Colchicine induced mutation and enhancement of the steviol glycoside content in Stevia rebaudiana / Nur Izzati Azizan

Stevia rebaudiana is a sweet herb that belongs to the family Asteraeae. Steviol glycoside, a natural sweetener derived from Stevia leaves are 100-300 times sweeter than sucrose and contain a complex mixture of sweet diterpene glycoside. the mutagenic effect of colchicine to improve leaf and steviol...

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Main Author: Azizan, Nur Izzati
Format: Thesis
Language:English
Published: 2020
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Online Access:https://ir.uitm.edu.my/id/eprint/60401/1/60401.pdf
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spelling my-uitm-ir.604012022-05-26T05:27:03Z Colchicine induced mutation and enhancement of the steviol glycoside content in Stevia rebaudiana / Nur Izzati Azizan 2020-12 Azizan, Nur Izzati Sugar crops. Sugar plants Stevia rebaudiana is a sweet herb that belongs to the family Asteraeae. Steviol glycoside, a natural sweetener derived from Stevia leaves are 100-300 times sweeter than sucrose and contain a complex mixture of sweet diterpene glycoside. the mutagenic effect of colchicine to improve leaf and steviol glycosides yield in stevia was investigated. Stevia plant were treated with five different colchicine concentrations (0.5%, 1.00%, 1.50%, 2.0%, 2.5% and 0.0% act as control) for 48h. The plants were arranged in a completely randomized block design. Treated plants were characterized based on morphological traits such as plant height, leaf length and leaf thickness. The results obtained revealed significant difference (p≤0.05) in the morphological traits of the mutants when compared with the control. Overall, 2.00% colchicine concentrations resulted in higher average plant height by 36.0% ±0.6 leaf length increments by 43.0%± 0.7 and the leaf thickness increment by 5.0%±0.1. The improvement of the mutants' traits is concentration dependent and increases with increment in colchicine concentration. Therefore it is suggested that 2.0% concentration should be applied in improving stevia growth and yield related traits. 2020-12 Thesis https://ir.uitm.edu.my/id/eprint/60401/ https://ir.uitm.edu.my/id/eprint/60401/1/60401.pdf text en public masters Universiti Teknologi MARA Faculty of Plantation Management and Agrotechnology Abdullah, Shamsiah (Associate Professor Ts. Dr.)
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
advisor Abdullah, Shamsiah (Associate Professor Ts. Dr.)
topic Sugar crops
Sugar plants
spellingShingle Sugar crops
Sugar plants
Azizan, Nur Izzati
Colchicine induced mutation and enhancement of the steviol glycoside content in Stevia rebaudiana / Nur Izzati Azizan
description Stevia rebaudiana is a sweet herb that belongs to the family Asteraeae. Steviol glycoside, a natural sweetener derived from Stevia leaves are 100-300 times sweeter than sucrose and contain a complex mixture of sweet diterpene glycoside. the mutagenic effect of colchicine to improve leaf and steviol glycosides yield in stevia was investigated. Stevia plant were treated with five different colchicine concentrations (0.5%, 1.00%, 1.50%, 2.0%, 2.5% and 0.0% act as control) for 48h. The plants were arranged in a completely randomized block design. Treated plants were characterized based on morphological traits such as plant height, leaf length and leaf thickness. The results obtained revealed significant difference (p≤0.05) in the morphological traits of the mutants when compared with the control. Overall, 2.00% colchicine concentrations resulted in higher average plant height by 36.0% ±0.6 leaf length increments by 43.0%± 0.7 and the leaf thickness increment by 5.0%±0.1. The improvement of the mutants' traits is concentration dependent and increases with increment in colchicine concentration. Therefore it is suggested that 2.0% concentration should be applied in improving stevia growth and yield related traits.
format Thesis
qualification_level Master's degree
author Azizan, Nur Izzati
author_facet Azizan, Nur Izzati
author_sort Azizan, Nur Izzati
title Colchicine induced mutation and enhancement of the steviol glycoside content in Stevia rebaudiana / Nur Izzati Azizan
title_short Colchicine induced mutation and enhancement of the steviol glycoside content in Stevia rebaudiana / Nur Izzati Azizan
title_full Colchicine induced mutation and enhancement of the steviol glycoside content in Stevia rebaudiana / Nur Izzati Azizan
title_fullStr Colchicine induced mutation and enhancement of the steviol glycoside content in Stevia rebaudiana / Nur Izzati Azizan
title_full_unstemmed Colchicine induced mutation and enhancement of the steviol glycoside content in Stevia rebaudiana / Nur Izzati Azizan
title_sort colchicine induced mutation and enhancement of the steviol glycoside content in stevia rebaudiana / nur izzati azizan
granting_institution Universiti Teknologi MARA
granting_department Faculty of Plantation Management and Agrotechnology
publishDate 2020
url https://ir.uitm.edu.my/id/eprint/60401/1/60401.pdf
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