Trichoderma asperellum as biological control agent for controlling black rot disease of pineapple var. MD2 in Malaysia / Nurul Farhana Mat Hayin

Malaysia has been on the list of the top 20 pineapple producing countries in the world. The success of MD2 variety in meeting the high demand in local and international markets has hastened the government to focus on fresh fruits production. However, postharvest losses of fresh fruits are common pro...

Full description

Saved in:

Bibliographic Details
Main Author:	Mat Hayin, Nurul Farhana
Format:	Thesis
Language:	English
Published:	2021
Subjects:	Pest control and treatment of diseases Plant protection
Online Access:	https://ir.uitm.edu.my/id/eprint/60402/1/60402.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uitm-ir.60402
record_format	uketd_dc
spelling	my-uitm-ir.604022022-05-26T06:08:37Z Trichoderma asperellum as biological control agent for controlling black rot disease of pineapple var. MD2 in Malaysia / Nurul Farhana Mat Hayin 2021-11 Mat Hayin, Nurul Farhana Pest control and treatment of diseases. Plant protection Malaysia has been on the list of the top 20 pineapple producing countries in the world. The success of MD2 variety in meeting the high demand in local and international markets has hastened the government to focus on fresh fruits production. However, postharvest losses of fresh fruits are common problems in developing countries, including Malaysia. One of several factors that contribute to the postharvest losses of fresh fruits is pathological disorders caused by pathogenic microorganisms. Black rot disease of pineapple is a postharvest disease caused by a facultative parasitic fungus known as Ceratocystis paradoxa (Dade) C. Moreau. This pathogen could tremendously shorten the shelf-life of MD2 pineapple during transportation and storage by making the flesh disintegrated, watery rot and eventually causing severe damage. To date, the role of chemical pesticides in controlling postharvest disease has been the subject of controversial debate. In this study, biological control agent plays a major role as a promising alternative method to control black rot disease of pineapple. A total of 17 isolates consisted of 13 different isolates of fungi, and four different isolates of bacteria were isolated from asymptomatic MD2 pineapple leaves and fruits. The antagonistic activities of 17 isolates against C. paradoxa were evaluated using a dual culture test. The most promising isolate with Percentage Inhibition of Radial Growth (PIRG) value of 97.5% was confirmed as Trichoderma asperellum (JX913783.1) using ITS rRNA gene. The assessment of disease severity index and disease progression analysis of MD2 pineapples were evaluated within ten days in the laboratory at room temperature, 25°C. After ten days, pineapples inoculated with C. paradoxa alone (positive control, T1) exhibited (100% severity index, 7.66 AUDPC units2, and 0% disease reduction), pineapples dipped in sterile distilled water (control, T2) exhibited (0% severity index, 0 AUDPC units2, and 100% disease reduction), pineapples dipped in 109 cfu/mL spore suspension of T. asperellum alone (negative control, T3) exhibited (0% severity index, 0 AUDPC units2, and 100% disease reduction), pineapples inoculated with C. paradoxa, followed by dipping in 109 cfu/mL spore suspension of T. asperellum after 4 hours (curative, T4) exhibited (100% severity index, 6.22 AUDPC units2, and 18.83% disease reduction) and pineapples dipped in 109 cfu/mL spore suspension of T. asperellum, followed by the inoculation of C. paradoxa after 4 hours (preventive, T5) exhibited (66.70% severity index, 4.56 AUDPC units2, and 40.57% disease reduction). The findings revealed the application of 109 cfu/mL spore suspension of T. asperellum 4 hours prior to pathogen inoculation (T5) was effective in preventing black rot disease in MD2 pineapples. This suggested the application of biological control agents prior to disease infection increases its effectiveness against postharvest pathogen(s). 2021-11 Thesis https://ir.uitm.edu.my/id/eprint/60402/ https://ir.uitm.edu.my/id/eprint/60402/1/60402.pdf text en public masters Universiti Teknologi MARA Faculty of Plantation Management and Agrotechnology Sapak, Zaiton (Ts. Dr.)
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Sapak, Zaiton (Ts. Dr.)
topic	Pest control and treatment of diseases Plant protection
spellingShingle	Pest control and treatment of diseases Plant protection Mat Hayin, Nurul Farhana Trichoderma asperellum as biological control agent for controlling black rot disease of pineapple var. MD2 in Malaysia / Nurul Farhana Mat Hayin
description	Malaysia has been on the list of the top 20 pineapple producing countries in the world. The success of MD2 variety in meeting the high demand in local and international markets has hastened the government to focus on fresh fruits production. However, postharvest losses of fresh fruits are common problems in developing countries, including Malaysia. One of several factors that contribute to the postharvest losses of fresh fruits is pathological disorders caused by pathogenic microorganisms. Black rot disease of pineapple is a postharvest disease caused by a facultative parasitic fungus known as Ceratocystis paradoxa (Dade) C. Moreau. This pathogen could tremendously shorten the shelf-life of MD2 pineapple during transportation and storage by making the flesh disintegrated, watery rot and eventually causing severe damage. To date, the role of chemical pesticides in controlling postharvest disease has been the subject of controversial debate. In this study, biological control agent plays a major role as a promising alternative method to control black rot disease of pineapple. A total of 17 isolates consisted of 13 different isolates of fungi, and four different isolates of bacteria were isolated from asymptomatic MD2 pineapple leaves and fruits. The antagonistic activities of 17 isolates against C. paradoxa were evaluated using a dual culture test. The most promising isolate with Percentage Inhibition of Radial Growth (PIRG) value of 97.5% was confirmed as Trichoderma asperellum (JX913783.1) using ITS rRNA gene. The assessment of disease severity index and disease progression analysis of MD2 pineapples were evaluated within ten days in the laboratory at room temperature, 25°C. After ten days, pineapples inoculated with C. paradoxa alone (positive control, T1) exhibited (100% severity index, 7.66 AUDPC units2, and 0% disease reduction), pineapples dipped in sterile distilled water (control, T2) exhibited (0% severity index, 0 AUDPC units2, and 100% disease reduction), pineapples dipped in 109 cfu/mL spore suspension of T. asperellum alone (negative control, T3) exhibited (0% severity index, 0 AUDPC units2, and 100% disease reduction), pineapples inoculated with C. paradoxa, followed by dipping in 109 cfu/mL spore suspension of T. asperellum after 4 hours (curative, T4) exhibited (100% severity index, 6.22 AUDPC units2, and 18.83% disease reduction) and pineapples dipped in 109 cfu/mL spore suspension of T. asperellum, followed by the inoculation of C. paradoxa after 4 hours (preventive, T5) exhibited (66.70% severity index, 4.56 AUDPC units2, and 40.57% disease reduction). The findings revealed the application of 109 cfu/mL spore suspension of T. asperellum 4 hours prior to pathogen inoculation (T5) was effective in preventing black rot disease in MD2 pineapples. This suggested the application of biological control agents prior to disease infection increases its effectiveness against postharvest pathogen(s).
format	Thesis
qualification_level	Master's degree
author	Mat Hayin, Nurul Farhana
author_facet	Mat Hayin, Nurul Farhana
author_sort	Mat Hayin, Nurul Farhana
title	Trichoderma asperellum as biological control agent for controlling black rot disease of pineapple var. MD2 in Malaysia / Nurul Farhana Mat Hayin
title_short	Trichoderma asperellum as biological control agent for controlling black rot disease of pineapple var. MD2 in Malaysia / Nurul Farhana Mat Hayin
title_full	Trichoderma asperellum as biological control agent for controlling black rot disease of pineapple var. MD2 in Malaysia / Nurul Farhana Mat Hayin
title_fullStr	Trichoderma asperellum as biological control agent for controlling black rot disease of pineapple var. MD2 in Malaysia / Nurul Farhana Mat Hayin
title_full_unstemmed	Trichoderma asperellum as biological control agent for controlling black rot disease of pineapple var. MD2 in Malaysia / Nurul Farhana Mat Hayin
title_sort	trichoderma asperellum as biological control agent for controlling black rot disease of pineapple var. md2 in malaysia / nurul farhana mat hayin
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Plantation Management and Agrotechnology
publishDate	2021
url	https://ir.uitm.edu.my/id/eprint/60402/1/60402.pdf
_version_	1783735125520941056

Trichoderma asperellum as biological control agent for controlling black rot disease of pineapple var. MD2 in Malaysia / Nurul Farhana Mat Hayin

Similar Items