Effects of Ficus deltoidea on metabolic and reproductive system in letrozole induced female polycystic ovarian syndrome rats / Muhammad Aliff Haslan

Polycystic ovarian syndrome (PCOS) affects fertility and leads to hormonal derangements in women. Ficus deltoidea Jack (Moraceae) has been reported capable of promoting fertility in PCOS rats. However, the exact mechanism underlying the therapeutic effects of F. deltoidea in alleviating PCOS symptom...

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Main Author:	Haslan, Muhammad Aliff
Format:	Thesis
Language:	English
Published:	2021
Subjects:	Medical botany (General)
Online Access:	https://ir.uitm.edu.my/id/eprint/60497/1/60497.pdf
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spelling	my-uitm-ir.604972022-05-30T02:48:28Z Effects of Ficus deltoidea on metabolic and reproductive system in letrozole induced female polycystic ovarian syndrome rats / Muhammad Aliff Haslan 2021-11 Haslan, Muhammad Aliff Medical botany (General) Polycystic ovarian syndrome (PCOS) affects fertility and leads to hormonal derangements in women. Ficus deltoidea Jack (Moraceae) has been reported capable of promoting fertility in PCOS rats. However, the exact mechanism underlying the therapeutic effects of F. deltoidea in alleviating PCOS symptoms remains elusive. Therefore, the study aimed to investigate the biochemical, hormonal, and histological changes in letrozole (LTZ) induced PCOS female rats following treatment with F. deltoidea extract (PFD). Five groups were induced into PCOS, while the sixth group was a non-PCOS normal control. PCOS induced groups were grouped and treated with: saline; 10 mg/kg b.wt clomiphene citrate; and plant extract treated animals PFD250, PFD500, and PFD1000 received 250 mg, 500 mg, and 1000 mg/kg b. wt respectively for 15 days. At the end of the treatment, the blood samples were collected for biochemical and hormonal analyses. The serum levels of fasting blood glucose, fasting insulin, total cholesterol, triglycerides, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol, testosterone, estrogen, progesterone, luteinizing hormone, and follicle-stimulating hormone were determined by ELISA assays. Histomorphometric changes in the ovarian and uterus tissue were observed by hematoxylin and eosin staining. The present study demonstrates, for the first time, that treatment with higher dosages of F. deltoidea extracts (500 mg and 1000 mg/kg bwt) towards LTZ induced PCOS rats showed significant (p<0.05) improvement on the physical parameters, lipid profiles, and glucose homeostasis. The reproductive system also demonstrated significant increased (p<0.05) in the estrous cyclicity and restoration on the steroid and gonadotropin hormones. A significant (p<0.05) improvement was also demonstrated on the ovarian histology, such as a higher number of corpus luteum, healthy follicles, indicating the ability of F. deltoidea to induce ovulation and a lesser amount of follicular cysts. Meanwhile, the uterine histology displayed an inreased in uterine and endometrial thickness, increasing the number of endometrial glands. Apart from this, the oxidative damages caused by PCOS were also encountered by increasing antioxidant levels. These findings suggest that treatment with F. deltoidea can reverse some symptoms of PCOS in female rats by improving glucose homeostasis, antioxidant activity, hormonal balance, and histological changes. 2021-11 Thesis https://ir.uitm.edu.my/id/eprint/60497/ https://ir.uitm.edu.my/id/eprint/60497/1/60497.pdf text en public masters Universiti Teknologi MARA Faculty of Applied Sciences Hashim, Nooraain (Dr.)
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Hashim, Nooraain (Dr.)
topic	Medical botany (General)
spellingShingle	Medical botany (General) Haslan, Muhammad Aliff Effects of Ficus deltoidea on metabolic and reproductive system in letrozole induced female polycystic ovarian syndrome rats / Muhammad Aliff Haslan
description	Polycystic ovarian syndrome (PCOS) affects fertility and leads to hormonal derangements in women. Ficus deltoidea Jack (Moraceae) has been reported capable of promoting fertility in PCOS rats. However, the exact mechanism underlying the therapeutic effects of F. deltoidea in alleviating PCOS symptoms remains elusive. Therefore, the study aimed to investigate the biochemical, hormonal, and histological changes in letrozole (LTZ) induced PCOS female rats following treatment with F. deltoidea extract (PFD). Five groups were induced into PCOS, while the sixth group was a non-PCOS normal control. PCOS induced groups were grouped and treated with: saline; 10 mg/kg b.wt clomiphene citrate; and plant extract treated animals PFD250, PFD500, and PFD1000 received 250 mg, 500 mg, and 1000 mg/kg b. wt respectively for 15 days. At the end of the treatment, the blood samples were collected for biochemical and hormonal analyses. The serum levels of fasting blood glucose, fasting insulin, total cholesterol, triglycerides, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol, testosterone, estrogen, progesterone, luteinizing hormone, and follicle-stimulating hormone were determined by ELISA assays. Histomorphometric changes in the ovarian and uterus tissue were observed by hematoxylin and eosin staining. The present study demonstrates, for the first time, that treatment with higher dosages of F. deltoidea extracts (500 mg and 1000 mg/kg bwt) towards LTZ induced PCOS rats showed significant (p<0.05) improvement on the physical parameters, lipid profiles, and glucose homeostasis. The reproductive system also demonstrated significant increased (p<0.05) in the estrous cyclicity and restoration on the steroid and gonadotropin hormones. A significant (p<0.05) improvement was also demonstrated on the ovarian histology, such as a higher number of corpus luteum, healthy follicles, indicating the ability of F. deltoidea to induce ovulation and a lesser amount of follicular cysts. Meanwhile, the uterine histology displayed an inreased in uterine and endometrial thickness, increasing the number of endometrial glands. Apart from this, the oxidative damages caused by PCOS were also encountered by increasing antioxidant levels. These findings suggest that treatment with F. deltoidea can reverse some symptoms of PCOS in female rats by improving glucose homeostasis, antioxidant activity, hormonal balance, and histological changes.
format	Thesis
qualification_level	Master's degree
author	Haslan, Muhammad Aliff
author_facet	Haslan, Muhammad Aliff
author_sort	Haslan, Muhammad Aliff
title	Effects of Ficus deltoidea on metabolic and reproductive system in letrozole induced female polycystic ovarian syndrome rats / Muhammad Aliff Haslan
title_short	Effects of Ficus deltoidea on metabolic and reproductive system in letrozole induced female polycystic ovarian syndrome rats / Muhammad Aliff Haslan
title_full	Effects of Ficus deltoidea on metabolic and reproductive system in letrozole induced female polycystic ovarian syndrome rats / Muhammad Aliff Haslan
title_fullStr	Effects of Ficus deltoidea on metabolic and reproductive system in letrozole induced female polycystic ovarian syndrome rats / Muhammad Aliff Haslan
title_full_unstemmed	Effects of Ficus deltoidea on metabolic and reproductive system in letrozole induced female polycystic ovarian syndrome rats / Muhammad Aliff Haslan
title_sort	effects of ficus deltoidea on metabolic and reproductive system in letrozole induced female polycystic ovarian syndrome rats / muhammad aliff haslan
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2021
url	https://ir.uitm.edu.my/id/eprint/60497/1/60497.pdf
_version_	1783735133991337984

Effects of Ficus deltoidea on metabolic and reproductive system in letrozole induced female polycystic ovarian syndrome rats / Muhammad Aliff Haslan

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