Glycoprotein profiling of Staphylococcus epidermidis and its role in biofilm formation and antibiotic resistance / Nur Alia Othman

Staphylococcus epidermidis is the most frequently detected coagulase-negative staphylococcus (CoNS) that is commonly responsible for catheter-related and central nervous system (CNS) infections. It is an opportunistic pathogen capable of forming biofilm which contributes to device-related infections...

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Main Author:	Othman, Nur Alia
Format:	Thesis
Language:	English
Published:	2021
Subjects:	Bacteria
Online Access:	https://ir.uitm.edu.my/id/eprint/60522/1/60522.pdf
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id	my-uitm-ir.60522
record_format	uketd_dc
spelling	my-uitm-ir.605222022-05-30T03:46:33Z Glycoprotein profiling of Staphylococcus epidermidis and its role in biofilm formation and antibiotic resistance / Nur Alia Othman 2021-11 Othman, Nur Alia Bacteria Staphylococcus epidermidis is the most frequently detected coagulase-negative staphylococcus (CoNS) that is commonly responsible for catheter-related and central nervous system (CNS) infections. It is an opportunistic pathogen capable of forming biofilm which contributes to device-related infections and resistance to antibiotics. The ability to form biofilm has been linked with glycoproteins in biofilm-producing bacteria. This study investigates the differential glycoprotein expression between the biofilm producer and nonbiofilm producer of S. epidermidis and also its involvement in enhancing antibiotic resistance of the biofilm. S. epidermidis strains were subjected to N- and O-glycosylation inhibition followed by physical characterisation by Field Emission Electron Scanning Microscopy (FESEM) and antibiotic resistance profiling. The absence of O-glycosylation ascertains with reduced biofilm production of ATCC35984 strain and biofilm thickness. Subsequently, treatment with vancomycin, rifampicin and tetracycline showed that the antibiotics were more effective against O-glycan deficient biofilm. Differential protein expression was observed in planktonic and biofilm cultures of both biofilm and nonbiofilm producing strains. Glycoproteins were detected using Pro-Q® Emerald 300 Glycoprotein staining and subjected to mass spectrometry (LC-MS) identification. Out of the 17 spots characterized, 55 glycoproteins were identified for glycoprotein site prediction as well as protein interaction analysis using STRING tool and Clusters of Orthologous Genes (COGs). Majority of these proteins were found to carry either N- or O-glycosylation sites with some known to be related with biofilm formation such as glutamate synthase and influence antibiotic resistance for example glutamate-tRNA ligase. Therefore, O-glycans are suggested to have potential impact on biofilm formation and antibiotic resistance of S. epidermidis which warrants further evaluation and validation. 2021-11 Thesis https://ir.uitm.edu.my/id/eprint/60522/ https://ir.uitm.edu.my/id/eprint/60522/1/60522.pdf text en public masters Universiti Teknologi MARA Faculty of Applied Sciences Abdul Hamid, Umi Marshida (Dr.)
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Abdul Hamid, Umi Marshida (Dr.)
topic	Bacteria
spellingShingle	Bacteria Othman, Nur Alia Glycoprotein profiling of Staphylococcus epidermidis and its role in biofilm formation and antibiotic resistance / Nur Alia Othman
description	Staphylococcus epidermidis is the most frequently detected coagulase-negative staphylococcus (CoNS) that is commonly responsible for catheter-related and central nervous system (CNS) infections. It is an opportunistic pathogen capable of forming biofilm which contributes to device-related infections and resistance to antibiotics. The ability to form biofilm has been linked with glycoproteins in biofilm-producing bacteria. This study investigates the differential glycoprotein expression between the biofilm producer and nonbiofilm producer of S. epidermidis and also its involvement in enhancing antibiotic resistance of the biofilm. S. epidermidis strains were subjected to N- and O-glycosylation inhibition followed by physical characterisation by Field Emission Electron Scanning Microscopy (FESEM) and antibiotic resistance profiling. The absence of O-glycosylation ascertains with reduced biofilm production of ATCC35984 strain and biofilm thickness. Subsequently, treatment with vancomycin, rifampicin and tetracycline showed that the antibiotics were more effective against O-glycan deficient biofilm. Differential protein expression was observed in planktonic and biofilm cultures of both biofilm and nonbiofilm producing strains. Glycoproteins were detected using Pro-Q® Emerald 300 Glycoprotein staining and subjected to mass spectrometry (LC-MS) identification. Out of the 17 spots characterized, 55 glycoproteins were identified for glycoprotein site prediction as well as protein interaction analysis using STRING tool and Clusters of Orthologous Genes (COGs). Majority of these proteins were found to carry either N- or O-glycosylation sites with some known to be related with biofilm formation such as glutamate synthase and influence antibiotic resistance for example glutamate-tRNA ligase. Therefore, O-glycans are suggested to have potential impact on biofilm formation and antibiotic resistance of S. epidermidis which warrants further evaluation and validation.
format	Thesis
qualification_level	Master's degree
author	Othman, Nur Alia
author_facet	Othman, Nur Alia
author_sort	Othman, Nur Alia
title	Glycoprotein profiling of Staphylococcus epidermidis and its role in biofilm formation and antibiotic resistance / Nur Alia Othman
title_short	Glycoprotein profiling of Staphylococcus epidermidis and its role in biofilm formation and antibiotic resistance / Nur Alia Othman
title_full	Glycoprotein profiling of Staphylococcus epidermidis and its role in biofilm formation and antibiotic resistance / Nur Alia Othman
title_fullStr	Glycoprotein profiling of Staphylococcus epidermidis and its role in biofilm formation and antibiotic resistance / Nur Alia Othman
title_full_unstemmed	Glycoprotein profiling of Staphylococcus epidermidis and its role in biofilm formation and antibiotic resistance / Nur Alia Othman
title_sort	glycoprotein profiling of staphylococcus epidermidis and its role in biofilm formation and antibiotic resistance / nur alia othman
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2021
url	https://ir.uitm.edu.my/id/eprint/60522/1/60522.pdf
_version_	1783735137978023936

Glycoprotein profiling of Staphylococcus epidermidis and its role in biofilm formation and antibiotic resistance / Nur Alia Othman

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