Mechanism of Aloe Emodin-Induced Apoptosis in ER+ - Breast Cancer Cells, MCF-7 / Indah Mohd Amin

Aloe emodin, an anthraquinone exhibits higher cytotoxicity to hepatoma, prostate and cervical cancer cells through cell cycle arrest and apoptosis compared to normal cells. However, its underlying mechanism on ER+ -breast cancer cell death remains unclear. Therefore, this study was done to investiga...

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Main Author:	Mohd Amin, Indah
Format:	Thesis
Language:	English
Published:	2016
Subjects:	Breast Mammary glands Diseases of the breast
Online Access:	https://ir.uitm.edu.my/id/eprint/62199/1/62199.pdf
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spelling	my-uitm-ir.621992022-06-27T11:36:59Z Mechanism of Aloe Emodin-Induced Apoptosis in ER+ - Breast Cancer Cells, MCF-7 / Indah Mohd Amin 2016-09 Mohd Amin, Indah Breast. Mammary glands Diseases of the breast Aloe emodin, an anthraquinone exhibits higher cytotoxicity to hepatoma, prostate and cervical cancer cells through cell cycle arrest and apoptosis compared to normal cells. However, its underlying mechanism on ER+ -breast cancer cell death remains unclear. Therefore, this study was done to investigate aloe emodin cytotoxicity and its mechanism on estrogen receptor (ER)-positive (MCF-7), ER-negative breast cancer cells (MDA-MB-231) and control breast cells (MCF-10A) in comparison with tamoxifen. Cytotoxicity was determined using WST-1 proliferation assay and Trypan blue exclusion test. Apoptosis mechanism was investigated using Annexin V-FITC/PI staining and DNA fragmentation assay. Both genes and proteins involved in the regulation of cell cycle (p53, p21, CDK1, CDK2, cyclin B1 and cyclin E1) and apoptosis (Fas, FADD, Caspase-3, Caspase-8, Caspase-9, Bax, Bcl-2, and Cytochrome c) in aloe emodin-treated MCF-7 were determined using Quantigene 2.0 Plex and protein ELISA assays respectively. Maximum treatment time was set up to 72 hours in all assays. Aloe emodin inhibited the proliferation of MCF-7 with IC₅₀ of 80μM. No IC₅₀ value was obtained on MDA-MB-231 and MCF-10A, even up to 150μM. In contrast, tamoxifen was non-selective to all cells with IC₅₀ of 27µM, 19μM and 42μM, respectively. IC₅₀ values obtained were used in all the other assays. Results from Trypan blue exclusion test were in concordance with the proliferation assay. Study on Annexin/PI staining showed the presence of early and late apoptosis (18.42% ± 3.53 to 29.25% ± 0.55; p<0.05, n=3 and 28.45% ± 2.36 to 30.22% ± 0.56; p>0.05, n=3, respectively) in aloe emodin and tamoxifen-treated MCF-7 cells. Accordingly, DNA fragmentation was observed. Aloe emodin and tamoxifen enhanced MCF-7 cytotoxicity through apoptosis. In cell cycle signalling, aloe emodin upregulated the expression of p53 and p21 proteins; while downregulating CDK1. Only CDK1 protein is in accordance with gene expression. In intrinsic apoptosis signalling, Bax, Cytochrome c and Caspase-9 proteins were upregulated; while no change observed in Bcl-2 protein. Except for Caspase-9, these results are in accordance with gene expression. In extrinsic apoptosis, Fas and Caspase-8 were upregulated, contrary to gene expressions. These findings indicate that aloe emodin cytotoxic action on MCF-7 cells is through G2/M arrest; both extrinsic and intrinsic apoptosis pathways. Its actions on G2/M phase arrest and activation of intrinsic apoptosis pathways were p53-dependent, while extrinsic apoptosis was p53independent. Data obtained suggests (i) aloe emodin has potential as a selective apoptotic inducer in ER+ -breast cancer management and (ii) and the present study could be used as a basic rationale for in vivo experiment setting. 2016-09 Thesis https://ir.uitm.edu.my/id/eprint/62199/ https://ir.uitm.edu.my/id/eprint/62199/1/62199.pdf text en public phd doctoral Universiti Teknologi MARA (Kampus Sg. Buloh) Faculty of Medicine Abdul Hamid Hasani, Narimah
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Abdul Hamid Hasani, Narimah
topic	Breast Mammary glands Diseases of the breast
spellingShingle	Breast Mammary glands Diseases of the breast Mohd Amin, Indah Mechanism of Aloe Emodin-Induced Apoptosis in ER+ - Breast Cancer Cells, MCF-7 / Indah Mohd Amin
description	Aloe emodin, an anthraquinone exhibits higher cytotoxicity to hepatoma, prostate and cervical cancer cells through cell cycle arrest and apoptosis compared to normal cells. However, its underlying mechanism on ER+ -breast cancer cell death remains unclear. Therefore, this study was done to investigate aloe emodin cytotoxicity and its mechanism on estrogen receptor (ER)-positive (MCF-7), ER-negative breast cancer cells (MDA-MB-231) and control breast cells (MCF-10A) in comparison with tamoxifen. Cytotoxicity was determined using WST-1 proliferation assay and Trypan blue exclusion test. Apoptosis mechanism was investigated using Annexin V-FITC/PI staining and DNA fragmentation assay. Both genes and proteins involved in the regulation of cell cycle (p53, p21, CDK1, CDK2, cyclin B1 and cyclin E1) and apoptosis (Fas, FADD, Caspase-3, Caspase-8, Caspase-9, Bax, Bcl-2, and Cytochrome c) in aloe emodin-treated MCF-7 were determined using Quantigene 2.0 Plex and protein ELISA assays respectively. Maximum treatment time was set up to 72 hours in all assays. Aloe emodin inhibited the proliferation of MCF-7 with IC₅₀ of 80μM. No IC₅₀ value was obtained on MDA-MB-231 and MCF-10A, even up to 150μM. In contrast, tamoxifen was non-selective to all cells with IC₅₀ of 27µM, 19μM and 42μM, respectively. IC₅₀ values obtained were used in all the other assays. Results from Trypan blue exclusion test were in concordance with the proliferation assay. Study on Annexin/PI staining showed the presence of early and late apoptosis (18.42% ± 3.53 to 29.25% ± 0.55; p<0.05, n=3 and 28.45% ± 2.36 to 30.22% ± 0.56; p>0.05, n=3, respectively) in aloe emodin and tamoxifen-treated MCF-7 cells. Accordingly, DNA fragmentation was observed. Aloe emodin and tamoxifen enhanced MCF-7 cytotoxicity through apoptosis. In cell cycle signalling, aloe emodin upregulated the expression of p53 and p21 proteins; while downregulating CDK1. Only CDK1 protein is in accordance with gene expression. In intrinsic apoptosis signalling, Bax, Cytochrome c and Caspase-9 proteins were upregulated; while no change observed in Bcl-2 protein. Except for Caspase-9, these results are in accordance with gene expression. In extrinsic apoptosis, Fas and Caspase-8 were upregulated, contrary to gene expressions. These findings indicate that aloe emodin cytotoxic action on MCF-7 cells is through G2/M arrest; both extrinsic and intrinsic apoptosis pathways. Its actions on G2/M phase arrest and activation of intrinsic apoptosis pathways were p53-dependent, while extrinsic apoptosis was p53independent. Data obtained suggests (i) aloe emodin has potential as a selective apoptotic inducer in ER+ -breast cancer management and (ii) and the present study could be used as a basic rationale for in vivo experiment setting.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Mohd Amin, Indah
author_facet	Mohd Amin, Indah
author_sort	Mohd Amin, Indah
title	Mechanism of Aloe Emodin-Induced Apoptosis in ER+ - Breast Cancer Cells, MCF-7 / Indah Mohd Amin
title_short	Mechanism of Aloe Emodin-Induced Apoptosis in ER+ - Breast Cancer Cells, MCF-7 / Indah Mohd Amin
title_full	Mechanism of Aloe Emodin-Induced Apoptosis in ER+ - Breast Cancer Cells, MCF-7 / Indah Mohd Amin
title_fullStr	Mechanism of Aloe Emodin-Induced Apoptosis in ER+ - Breast Cancer Cells, MCF-7 / Indah Mohd Amin
title_full_unstemmed	Mechanism of Aloe Emodin-Induced Apoptosis in ER+ - Breast Cancer Cells, MCF-7 / Indah Mohd Amin
title_sort	mechanism of aloe emodin-induced apoptosis in er+ - breast cancer cells, mcf-7 / indah mohd amin
granting_institution	Universiti Teknologi MARA (Kampus Sg. Buloh)
granting_department	Faculty of Medicine
publishDate	2016
url	https://ir.uitm.edu.my/id/eprint/62199/1/62199.pdf
_version_	1783735248954064896

Mechanism of Aloe Emodin-Induced Apoptosis in ER+ - Breast Cancer Cells, MCF-7 / Indah Mohd Amin

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