Reprogramming foreskin fibroblast cells from haemophilia Type-A patients into induced pluripotent stem cells / Noor Sabirah Husin

Induced Pluripotent Stem Cells (iPS cells) has an enormous potential in clinical application for example in the modelling of haemophilia. Studies on human pluripotent stem cells give hope to formulate potential cellular therapies for disease modelling. This study was designed to reprogram fibroblast...

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Main Author:	Husin, Noor Sabirah
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Cell populations Diseases of the blood and blood-forming organs
Online Access:	https://ir.uitm.edu.my/id/eprint/62283/1/62283.pdf
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id	my-uitm-ir.62283
record_format	uketd_dc
spelling	my-uitm-ir.622832022-10-25T03:12:56Z Reprogramming foreskin fibroblast cells from haemophilia Type-A patients into induced pluripotent stem cells / Noor Sabirah Husin 2017-11 Husin, Noor Sabirah Cell populations Diseases of the blood and blood-forming organs Induced Pluripotent Stem Cells (iPS cells) has an enormous potential in clinical application for example in the modelling of haemophilia. Studies on human pluripotent stem cells give hope to formulate potential cellular therapies for disease modelling. This study was designed to reprogram fibroblast cells from haemophilia type-A patients into iPS cells using four different techniques. Enzyme dissociation and explant culture technique were used to isolate primary cell fibroblast from haemophilia type-A patient. The enzymatic dissociation technique required three days to develop into fibroblast cells compared to explant culture which required 10 days in culture. Properties of the iPS cells depended on the reprogramming method. Morphological characteristics of iPS cells were observed only in Stemgent mRNA and Neon ® Reprogramming techniques. Expressions of pluripotency were verified using pluripotency markers. Downregulated and upregulated gene expression were analysed using qRT-PCR. This study provides new information on the development of haemophilia modelling, based on patientspecific iPS cells using integration-free methods. 2017-11 Thesis https://ir.uitm.edu.my/id/eprint/62283/ https://ir.uitm.edu.my/id/eprint/62283/1/62283.pdf text en public masters Universiti Teknologi MARA (Kampus Sg. Buloh) Faculty of Medicine Abdul Latiff, Amir Muhriz
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Abdul Latiff, Amir Muhriz
topic	Cell populations Diseases of the blood and blood-forming organs
spellingShingle	Cell populations Diseases of the blood and blood-forming organs Husin, Noor Sabirah Reprogramming foreskin fibroblast cells from haemophilia Type-A patients into induced pluripotent stem cells / Noor Sabirah Husin
description	Induced Pluripotent Stem Cells (iPS cells) has an enormous potential in clinical application for example in the modelling of haemophilia. Studies on human pluripotent stem cells give hope to formulate potential cellular therapies for disease modelling. This study was designed to reprogram fibroblast cells from haemophilia type-A patients into iPS cells using four different techniques. Enzyme dissociation and explant culture technique were used to isolate primary cell fibroblast from haemophilia type-A patient. The enzymatic dissociation technique required three days to develop into fibroblast cells compared to explant culture which required 10 days in culture. Properties of the iPS cells depended on the reprogramming method. Morphological characteristics of iPS cells were observed only in Stemgent mRNA and Neon ® Reprogramming techniques. Expressions of pluripotency were verified using pluripotency markers. Downregulated and upregulated gene expression were analysed using qRT-PCR. This study provides new information on the development of haemophilia modelling, based on patientspecific iPS cells using integration-free methods.
format	Thesis
qualification_level	Master's degree
author	Husin, Noor Sabirah
author_facet	Husin, Noor Sabirah
author_sort	Husin, Noor Sabirah
title	Reprogramming foreskin fibroblast cells from haemophilia Type-A patients into induced pluripotent stem cells / Noor Sabirah Husin
title_short	Reprogramming foreskin fibroblast cells from haemophilia Type-A patients into induced pluripotent stem cells / Noor Sabirah Husin
title_full	Reprogramming foreskin fibroblast cells from haemophilia Type-A patients into induced pluripotent stem cells / Noor Sabirah Husin
title_fullStr	Reprogramming foreskin fibroblast cells from haemophilia Type-A patients into induced pluripotent stem cells / Noor Sabirah Husin
title_full_unstemmed	Reprogramming foreskin fibroblast cells from haemophilia Type-A patients into induced pluripotent stem cells / Noor Sabirah Husin
title_sort	reprogramming foreskin fibroblast cells from haemophilia type-a patients into induced pluripotent stem cells / noor sabirah husin
granting_institution	Universiti Teknologi MARA (Kampus Sg. Buloh)
granting_department	Faculty of Medicine
publishDate	2017
url	https://ir.uitm.edu.my/id/eprint/62283/1/62283.pdf
_version_	1783735251015565312

Reprogramming foreskin fibroblast cells from haemophilia Type-A patients into induced pluripotent stem cells / Noor Sabirah Husin

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