The effect of leptin on utero-placental expression of EPHB4, Ephrin-B1, Ephrin-B2 and blood pressure in Sprague Dawley Rats / Norizan Kamal Basah

Background: Ephs and ephrins might play a role in the migration of cytotrophoblast during endometrial invasion. Objective: This study attempts to establish the pattern of expression of EPHB4, Ephrin-B1 and Ephrin-B2 in the utero-placental unit during pregnancy in control and leptin-treated rats. Met...

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Main Author:	Kamal Basah, Norizan
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Chordates Vertebrates Organic substances Diseases of the circulatory (Cardiovascular) system
Online Access:	https://ir.uitm.edu.my/id/eprint/62407/1/62407.pdf
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spelling	my-uitm-ir.624072022-06-28T07:59:53Z The effect of leptin on utero-placental expression of EPHB4, Ephrin-B1, Ephrin-B2 and blood pressure in Sprague Dawley Rats / Norizan Kamal Basah 2017-07 Kamal Basah, Norizan Chordates. Vertebrates Organic substances Diseases of the circulatory (Cardiovascular) system Background: Ephs and ephrins might play a role in the migration of cytotrophoblast during endometrial invasion. Objective: This study attempts to establish the pattern of expression of EPHB4, Ephrin-B1 and Ephrin-B2 in the utero-placental unit during pregnancy in control and leptin-treated rats. Method: Normotensive female Sprague Dawley rats, aged 12 weeks in pro-estrous were individually housed overnight with a male. Upon confirmation of mating the rats were randomized into two groups; control group and leptin-treated group. These groups were further divided into 10 subgroups (n=6 per group). Rats in the leptin-treated groups were given daily 60 µg/kg BW/day of leptin subcutaneously from two weeks prior to mating and until the day they were euthanized. Rats were euthanized every 2 days from day 5 of pregnancy and the placentae were collected for RT-PCR and immunohistochemistry. Data were analyzed using two-way ANOVA and expressed as mean ± SEM. Result: Results showed that Ephrin-B1 and EPHB4 expressions in the placenta and uterus decreased significantly from early to mid-pregnancy and then increased towards the end of pregnancy in normal rats (p<0.001). Ephrin-B2 expression decreased over the course of pregnancy in both the placenta and uterus (p<0.001). Ephrin-B1 expression was significantly higher in the leptin-treated group at day 21 of pregnancy. It appears that Ephrins-B1, B2 and EPHB4 are expressed by both the placenta and the uterus of the rat and their expression varies along the course of pregnancy. Conclusion: In conclusion, although the expression of Ephrin-B1 is affected by leptin, it might not have a role in leptin induced hypertension during pregnancy in the rats. 2017-07 Thesis https://ir.uitm.edu.my/id/eprint/62407/ https://ir.uitm.edu.my/id/eprint/62407/1/62407.pdf text en public masters Universiti Teknologi MARA (Kampus Sg. Buloh) Faculty of Medicine Sergey, Gupalo
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Sergey, Gupalo
topic	Chordates Vertebrates Organic substances Diseases of the circulatory (Cardiovascular) system
spellingShingle	Chordates Vertebrates Organic substances Diseases of the circulatory (Cardiovascular) system Kamal Basah, Norizan The effect of leptin on utero-placental expression of EPHB4, Ephrin-B1, Ephrin-B2 and blood pressure in Sprague Dawley Rats / Norizan Kamal Basah
description	Background: Ephs and ephrins might play a role in the migration of cytotrophoblast during endometrial invasion. Objective: This study attempts to establish the pattern of expression of EPHB4, Ephrin-B1 and Ephrin-B2 in the utero-placental unit during pregnancy in control and leptin-treated rats. Method: Normotensive female Sprague Dawley rats, aged 12 weeks in pro-estrous were individually housed overnight with a male. Upon confirmation of mating the rats were randomized into two groups; control group and leptin-treated group. These groups were further divided into 10 subgroups (n=6 per group). Rats in the leptin-treated groups were given daily 60 µg/kg BW/day of leptin subcutaneously from two weeks prior to mating and until the day they were euthanized. Rats were euthanized every 2 days from day 5 of pregnancy and the placentae were collected for RT-PCR and immunohistochemistry. Data were analyzed using two-way ANOVA and expressed as mean ± SEM. Result: Results showed that Ephrin-B1 and EPHB4 expressions in the placenta and uterus decreased significantly from early to mid-pregnancy and then increased towards the end of pregnancy in normal rats (p<0.001). Ephrin-B2 expression decreased over the course of pregnancy in both the placenta and uterus (p<0.001). Ephrin-B1 expression was significantly higher in the leptin-treated group at day 21 of pregnancy. It appears that Ephrins-B1, B2 and EPHB4 are expressed by both the placenta and the uterus of the rat and their expression varies along the course of pregnancy. Conclusion: In conclusion, although the expression of Ephrin-B1 is affected by leptin, it might not have a role in leptin induced hypertension during pregnancy in the rats.
format	Thesis
qualification_level	Master's degree
author	Kamal Basah, Norizan
author_facet	Kamal Basah, Norizan
author_sort	Kamal Basah, Norizan
title	The effect of leptin on utero-placental expression of EPHB4, Ephrin-B1, Ephrin-B2 and blood pressure in Sprague Dawley Rats / Norizan Kamal Basah
title_short	The effect of leptin on utero-placental expression of EPHB4, Ephrin-B1, Ephrin-B2 and blood pressure in Sprague Dawley Rats / Norizan Kamal Basah
title_full	The effect of leptin on utero-placental expression of EPHB4, Ephrin-B1, Ephrin-B2 and blood pressure in Sprague Dawley Rats / Norizan Kamal Basah
title_fullStr	The effect of leptin on utero-placental expression of EPHB4, Ephrin-B1, Ephrin-B2 and blood pressure in Sprague Dawley Rats / Norizan Kamal Basah
title_full_unstemmed	The effect of leptin on utero-placental expression of EPHB4, Ephrin-B1, Ephrin-B2 and blood pressure in Sprague Dawley Rats / Norizan Kamal Basah
title_sort	effect of leptin on utero-placental expression of ephb4, ephrin-b1, ephrin-b2 and blood pressure in sprague dawley rats / norizan kamal basah
granting_institution	Universiti Teknologi MARA (Kampus Sg. Buloh)
granting_department	Faculty of Medicine
publishDate	2017
url	https://ir.uitm.edu.my/id/eprint/62407/1/62407.pdf
_version_	1783735254359474176

The effect of leptin on utero-placental expression of EPHB4, Ephrin-B1, Ephrin-B2 and blood pressure in Sprague Dawley Rats / Norizan Kamal Basah

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