Antiviral activity of N-substituted 5-(Phenylamino) uracil derivatives against chikungunya virus / Noor Farah Omar Ahmad

Chikungunya virus (CHIKV), is an arthropod-borne disease that causes Chikungunya fever. Currently, there is no available drug or vaccine to treat the infected CHIKV patients. Based on literature, novel N-substituted 5-(phenylamino)uracil derivatives exhibit inhibitory effects against HIV and Hepatit...

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Main Author:	Omar Ahmad, Noor Farah
Format:	Thesis
Language:	English
Published:	2018
Subjects:	Communicable diseases and public health Infectious and parasitic diseases
Online Access:	https://ir.uitm.edu.my/id/eprint/63344/1/63344.pdf
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id	my-uitm-ir.63344
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spelling	my-uitm-ir.633442022-12-01T04:34:08Z Antiviral activity of N-substituted 5-(Phenylamino) uracil derivatives against chikungunya virus / Noor Farah Omar Ahmad 2018-04 Omar Ahmad, Noor Farah Communicable diseases and public health Infectious and parasitic diseases Chikungunya virus (CHIKV), is an arthropod-borne disease that causes Chikungunya fever. Currently, there is no available drug or vaccine to treat the infected CHIKV patients. Based on literature, novel N-substituted 5-(phenylamino)uracil derivatives exhibit inhibitory effects against HIV and Hepatitis C virus but not yet been tested on CHIKV. The half maximal cytotoxic concentration (CC₅₀) of six 5-substituted(phenylamino) uracil and five 2,4-dioxo-3,4-dihydropyrimidine acetic acid compounds were at 200 µM and 800 µM respectively. Two compounds (Z214 and Z364) exhibited the best antiviral activity at concentration of 50 μM and 100 μM. Time-addition assay revealed that the inhibition was most efficient when Z214 (50 µΜ) and Z364 (100 μM) were added at 4 hour of post-infection (hpi) and at 6 hpi. This suggests that, these compounds have inhibitory effect as anti-CHIKV inhibitors at post-entry step of CHIKV replication cycle. Prophylactic treatment showed a 50 decrease in number of CHIKV plaques when Z214 (50 µM) and Z364 (100 μM) were added 5 hours before infection by 100% and 71% ± 7.01 respectively. Z214 and Z354 exhibited a significant effect against CHIKV attachment and adsorption to the Vero cells at all tested concentrations (1.56 µM to 100 µM) as compared to the virus control. Both compounds exhibited inhibition against CHIKV internalization when the compounds (at all tested concentration ranging from 1.56 µM to 100 µM) were added during virus internalization. In conclusion, these compounds under novel Nsubstituted 5-(phenylamino)uracil derivatives exhibited promising antiviral activity for Chikungunya virus and it could be further studied. 2018-04 Thesis https://ir.uitm.edu.my/id/eprint/63344/ https://ir.uitm.edu.my/id/eprint/63344/1/63344.pdf text en public masters Universiti Teknologi MARA (Kampus Sg. Buloh) Faculty of Medicine Wang, Seok Mui
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Wang, Seok Mui
topic	Communicable diseases and public health Infectious and parasitic diseases
spellingShingle	Communicable diseases and public health Infectious and parasitic diseases Omar Ahmad, Noor Farah Antiviral activity of N-substituted 5-(Phenylamino) uracil derivatives against chikungunya virus / Noor Farah Omar Ahmad
description	Chikungunya virus (CHIKV), is an arthropod-borne disease that causes Chikungunya fever. Currently, there is no available drug or vaccine to treat the infected CHIKV patients. Based on literature, novel N-substituted 5-(phenylamino)uracil derivatives exhibit inhibitory effects against HIV and Hepatitis C virus but not yet been tested on CHIKV. The half maximal cytotoxic concentration (CC₅₀) of six 5-substituted(phenylamino) uracil and five 2,4-dioxo-3,4-dihydropyrimidine acetic acid compounds were at 200 µM and 800 µM respectively. Two compounds (Z214 and Z364) exhibited the best antiviral activity at concentration of 50 μM and 100 μM. Time-addition assay revealed that the inhibition was most efficient when Z214 (50 µΜ) and Z364 (100 μM) were added at 4 hour of post-infection (hpi) and at 6 hpi. This suggests that, these compounds have inhibitory effect as anti-CHIKV inhibitors at post-entry step of CHIKV replication cycle. Prophylactic treatment showed a 50 decrease in number of CHIKV plaques when Z214 (50 µM) and Z364 (100 μM) were added 5 hours before infection by 100% and 71% ± 7.01 respectively. Z214 and Z354 exhibited a significant effect against CHIKV attachment and adsorption to the Vero cells at all tested concentrations (1.56 µM to 100 µM) as compared to the virus control. Both compounds exhibited inhibition against CHIKV internalization when the compounds (at all tested concentration ranging from 1.56 µM to 100 µM) were added during virus internalization. In conclusion, these compounds under novel Nsubstituted 5-(phenylamino)uracil derivatives exhibited promising antiviral activity for Chikungunya virus and it could be further studied.
format	Thesis
qualification_level	Master's degree
author	Omar Ahmad, Noor Farah
author_facet	Omar Ahmad, Noor Farah
author_sort	Omar Ahmad, Noor Farah
title	Antiviral activity of N-substituted 5-(Phenylamino) uracil derivatives against chikungunya virus / Noor Farah Omar Ahmad
title_short	Antiviral activity of N-substituted 5-(Phenylamino) uracil derivatives against chikungunya virus / Noor Farah Omar Ahmad
title_full	Antiviral activity of N-substituted 5-(Phenylamino) uracil derivatives against chikungunya virus / Noor Farah Omar Ahmad
title_fullStr	Antiviral activity of N-substituted 5-(Phenylamino) uracil derivatives against chikungunya virus / Noor Farah Omar Ahmad
title_full_unstemmed	Antiviral activity of N-substituted 5-(Phenylamino) uracil derivatives against chikungunya virus / Noor Farah Omar Ahmad
title_sort	antiviral activity of n-substituted 5-(phenylamino) uracil derivatives against chikungunya virus / noor farah omar ahmad
granting_institution	Universiti Teknologi MARA (Kampus Sg. Buloh)
granting_department	Faculty of Medicine
publishDate	2018
url	https://ir.uitm.edu.my/id/eprint/63344/1/63344.pdf
_version_	1783735317730164736

Antiviral activity of N-substituted 5-(Phenylamino) uracil derivatives against chikungunya virus / Noor Farah Omar Ahmad

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