Anti-Inflammation effect of Genistein and Daidzein on Oxidised Low-Density Lipoprotein-Stimulated Human Coronary Artery Endothelial Cells / Lina Izzati Amer

Oxidised low-density lipoprotein (OxLDL) exerts adverse reaction to the endothelial cells in chronic oxidative disorder like atherosclerosis. Adhesion molecules such as VCAM-1 and ICAM-1, are regarded as key inflammatory markers promoting the endothelial cell injury, whereas nitric oxide regulates t...

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Main Author:	Amer, Lina Izzati
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Organic substances Diseases of the circulatory (Cardiovascular) system
Online Access:	https://ir.uitm.edu.my/id/eprint/63510/2/63510.pdf
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id	my-uitm-ir.63510
record_format	uketd_dc
spelling	my-uitm-ir.635102024-07-10T07:09:41Z Anti-Inflammation effect of Genistein and Daidzein on Oxidised Low-Density Lipoprotein-Stimulated Human Coronary Artery Endothelial Cells / Lina Izzati Amer 2019-10 Amer, Lina Izzati Organic substances Diseases of the circulatory (Cardiovascular) system Oxidised low-density lipoprotein (OxLDL) exerts adverse reaction to the endothelial cells in chronic oxidative disorder like atherosclerosis. Adhesion molecules such as VCAM-1 and ICAM-1, are regarded as key inflammatory markers promoting the endothelial cell injury, whereas nitric oxide regulates the disease process under OxLDL exposure. Isoflavones, genistein and daidzein, possess anti-inflammatory effects to improve the endothelial deterioration. However, the effects of genistein and daidzein on OxLDL stimulated endothelial injury and its inflammatory changes remain unexplored. In this study, the anti-inflammation role of genistein and daidzein on human coronary artery endothelial cells (HCAECs) under OxLDL exposure was investigated. The cells stimulated with 100 μg/mL of OxLDL showed reduced in cell viability rate. Different concentrations of genistein and daidzein were used as treatment for OxLDL stimulated HCAECs. VCAM-1 and ICAM-1 along with endothelial nitric oxide synthase (eNOS), were analysed quantitatively by expression of ELISA and supported by the immunofluorescence staining. Following treatment with low concentration of genistein at 6.25 µg/ml and high concentrations of daidzein at 12.5 and 25 µg/ml showed lower expression of VCAM-1 and ICAM-1 which regulated the progression of OxLDL-stimulated endothelial injury in HCAECs. Increased level of eNOS was observed in genistein and daidzein treated groups when compared to the untreated OxLDL group. Our study showed that genistein and daidzein, improved the endothelial cell viability, eNOS production and reduced inflammatory reactions under OxLDL exposure. Thus, genistein and daidzein were believed to have potential therapeutic effect in rescuing endothelial cells against OxLDL. 2019-10 Thesis https://ir.uitm.edu.my/id/eprint/63510/ https://ir.uitm.edu.my/id/eprint/63510/2/63510.pdf text en public masters Universiti Teknologi MARA (Kampus Sg. Buloh) Faculty of Medicine Syed Ahmad Fuad, Syed Baharom
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Syed Ahmad Fuad, Syed Baharom
topic	Organic substances Diseases of the circulatory (Cardiovascular) system
spellingShingle	Organic substances Diseases of the circulatory (Cardiovascular) system Amer, Lina Izzati Anti-Inflammation effect of Genistein and Daidzein on Oxidised Low-Density Lipoprotein-Stimulated Human Coronary Artery Endothelial Cells / Lina Izzati Amer
description	Oxidised low-density lipoprotein (OxLDL) exerts adverse reaction to the endothelial cells in chronic oxidative disorder like atherosclerosis. Adhesion molecules such as VCAM-1 and ICAM-1, are regarded as key inflammatory markers promoting the endothelial cell injury, whereas nitric oxide regulates the disease process under OxLDL exposure. Isoflavones, genistein and daidzein, possess anti-inflammatory effects to improve the endothelial deterioration. However, the effects of genistein and daidzein on OxLDL stimulated endothelial injury and its inflammatory changes remain unexplored. In this study, the anti-inflammation role of genistein and daidzein on human coronary artery endothelial cells (HCAECs) under OxLDL exposure was investigated. The cells stimulated with 100 μg/mL of OxLDL showed reduced in cell viability rate. Different concentrations of genistein and daidzein were used as treatment for OxLDL stimulated HCAECs. VCAM-1 and ICAM-1 along with endothelial nitric oxide synthase (eNOS), were analysed quantitatively by expression of ELISA and supported by the immunofluorescence staining. Following treatment with low concentration of genistein at 6.25 µg/ml and high concentrations of daidzein at 12.5 and 25 µg/ml showed lower expression of VCAM-1 and ICAM-1 which regulated the progression of OxLDL-stimulated endothelial injury in HCAECs. Increased level of eNOS was observed in genistein and daidzein treated groups when compared to the untreated OxLDL group. Our study showed that genistein and daidzein, improved the endothelial cell viability, eNOS production and reduced inflammatory reactions under OxLDL exposure. Thus, genistein and daidzein were believed to have potential therapeutic effect in rescuing endothelial cells against OxLDL.
format	Thesis
qualification_level	Master's degree
author	Amer, Lina Izzati
author_facet	Amer, Lina Izzati
author_sort	Amer, Lina Izzati
title	Anti-Inflammation effect of Genistein and Daidzein on Oxidised Low-Density Lipoprotein-Stimulated Human Coronary Artery Endothelial Cells / Lina Izzati Amer
title_short	Anti-Inflammation effect of Genistein and Daidzein on Oxidised Low-Density Lipoprotein-Stimulated Human Coronary Artery Endothelial Cells / Lina Izzati Amer
title_full	Anti-Inflammation effect of Genistein and Daidzein on Oxidised Low-Density Lipoprotein-Stimulated Human Coronary Artery Endothelial Cells / Lina Izzati Amer
title_fullStr	Anti-Inflammation effect of Genistein and Daidzein on Oxidised Low-Density Lipoprotein-Stimulated Human Coronary Artery Endothelial Cells / Lina Izzati Amer
title_full_unstemmed	Anti-Inflammation effect of Genistein and Daidzein on Oxidised Low-Density Lipoprotein-Stimulated Human Coronary Artery Endothelial Cells / Lina Izzati Amer
title_sort	anti-inflammation effect of genistein and daidzein on oxidised low-density lipoprotein-stimulated human coronary artery endothelial cells / lina izzati amer
granting_institution	Universiti Teknologi MARA (Kampus Sg. Buloh)
granting_department	Faculty of Medicine
publishDate	2019
url	https://ir.uitm.edu.my/id/eprint/63510/2/63510.pdf
_version_	1804889651322814464

Anti-Inflammation effect of Genistein and Daidzein on Oxidised Low-Density Lipoprotein-Stimulated Human Coronary Artery Endothelial Cells / Lina Izzati Amer

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