Molecular subtyping of Blastocystis sp. isolates from humans and animals in the Senoi and Proto-Malay tribes of Orang Asli, Pahang / Nabilah Amelia Mohammad

Infection by the parasite with unsettled clinical significance; Blastocystis is a common health problem in developing countries. The genetically diverse parasite currently classified into 17 subtypes (STs) with human isolates categorised into ST1 to ST9. Infections with genotypic homology animal iso...

Full description

Saved in:

Bibliographic Details
Main Author:	Mohammad, Nabilah Amelia
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Indigenous peoples Threatened societies
Online Access:	https://ir.uitm.edu.my/id/eprint/64116/1/64116.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uitm-ir.64116
record_format	uketd_dc
spelling	my-uitm-ir.641162023-01-27T02:47:18Z Molecular subtyping of Blastocystis sp. isolates from humans and animals in the Senoi and Proto-Malay tribes of Orang Asli, Pahang / Nabilah Amelia Mohammad 2017 Mohammad, Nabilah Amelia Indigenous peoples. Threatened societies Infection by the parasite with unsettled clinical significance; Blastocystis is a common health problem in developing countries. The genetically diverse parasite currently classified into 17 subtypes (STs) with human isolates categorised into ST1 to ST9. Infections with genotypic homology animal isolates are demonstrated in all of these nine subtypes. Hence, this study was undertaken to comprehensively determine the subtype distributions, zoonotic potential and pathogenicity of the parasite in 359 stool samples obtained from the Senoi and Proto-Malay tribes and animals from Sungai Lembing, Pahang. Samples were examined for the presence of Blastocystis using microscopic techniques (Wheatley's trichrome stain and in-vitro culture) and PCR assay. Demographic and socioeconomic data were collected using standardised questionnaire. Amplicons of 550 to 585-bp of the SSU rRNA gene were purified, sequenced and phylogenetic tree was constructed using maximum likelihood method in MEGA6. Among Orang Asli isolates, ST3 (9.5%) was the predominant subtype, followed by ST1 (5.5%), and ST2 (2.8%). ST2 (1.0%) and ST10 (11.3%) were detected in macaque and deer, respectively. ST3 was commonly found in symptomatic (6) and asymptomatic (18) individuals, thus, its pathogenic potential remains controversial. These findings deduced that Blastocystis is still prevalent among Orang Asli and animals. It also highlights the possible zoonotic risks as ST2 was found in both hosts. In addition, combination of both microscopic techniques are more superior (K = 0.353) than individual technique. Nevertheless, these informations may be beneficial to reassess the existing intervention strategies. It is hoped to prevent future expansion of the parasite hence significantly improve the quality of life and health of Orang Asli. 2017 Thesis https://ir.uitm.edu.my/id/eprint/64116/ https://ir.uitm.edu.my/id/eprint/64116/1/64116.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Health Sciences Tengku Ahmad Basri, Tengku Shahrul Anuar
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Tengku Ahmad Basri, Tengku Shahrul Anuar
topic	Indigenous peoples Threatened societies
spellingShingle	Indigenous peoples Threatened societies Mohammad, Nabilah Amelia Molecular subtyping of Blastocystis sp. isolates from humans and animals in the Senoi and Proto-Malay tribes of Orang Asli, Pahang / Nabilah Amelia Mohammad
description	Infection by the parasite with unsettled clinical significance; Blastocystis is a common health problem in developing countries. The genetically diverse parasite currently classified into 17 subtypes (STs) with human isolates categorised into ST1 to ST9. Infections with genotypic homology animal isolates are demonstrated in all of these nine subtypes. Hence, this study was undertaken to comprehensively determine the subtype distributions, zoonotic potential and pathogenicity of the parasite in 359 stool samples obtained from the Senoi and Proto-Malay tribes and animals from Sungai Lembing, Pahang. Samples were examined for the presence of Blastocystis using microscopic techniques (Wheatley's trichrome stain and in-vitro culture) and PCR assay. Demographic and socioeconomic data were collected using standardised questionnaire. Amplicons of 550 to 585-bp of the SSU rRNA gene were purified, sequenced and phylogenetic tree was constructed using maximum likelihood method in MEGA6. Among Orang Asli isolates, ST3 (9.5%) was the predominant subtype, followed by ST1 (5.5%), and ST2 (2.8%). ST2 (1.0%) and ST10 (11.3%) were detected in macaque and deer, respectively. ST3 was commonly found in symptomatic (6) and asymptomatic (18) individuals, thus, its pathogenic potential remains controversial. These findings deduced that Blastocystis is still prevalent among Orang Asli and animals. It also highlights the possible zoonotic risks as ST2 was found in both hosts. In addition, combination of both microscopic techniques are more superior (K = 0.353) than individual technique. Nevertheless, these informations may be beneficial to reassess the existing intervention strategies. It is hoped to prevent future expansion of the parasite hence significantly improve the quality of life and health of Orang Asli.
format	Thesis
qualification_level	Master's degree
author	Mohammad, Nabilah Amelia
author_facet	Mohammad, Nabilah Amelia
author_sort	Mohammad, Nabilah Amelia
title	Molecular subtyping of Blastocystis sp. isolates from humans and animals in the Senoi and Proto-Malay tribes of Orang Asli, Pahang / Nabilah Amelia Mohammad
title_short	Molecular subtyping of Blastocystis sp. isolates from humans and animals in the Senoi and Proto-Malay tribes of Orang Asli, Pahang / Nabilah Amelia Mohammad
title_full	Molecular subtyping of Blastocystis sp. isolates from humans and animals in the Senoi and Proto-Malay tribes of Orang Asli, Pahang / Nabilah Amelia Mohammad
title_fullStr	Molecular subtyping of Blastocystis sp. isolates from humans and animals in the Senoi and Proto-Malay tribes of Orang Asli, Pahang / Nabilah Amelia Mohammad
title_full_unstemmed	Molecular subtyping of Blastocystis sp. isolates from humans and animals in the Senoi and Proto-Malay tribes of Orang Asli, Pahang / Nabilah Amelia Mohammad
title_sort	molecular subtyping of blastocystis sp. isolates from humans and animals in the senoi and proto-malay tribes of orang asli, pahang / nabilah amelia mohammad
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Health Sciences
publishDate	2017
url	https://ir.uitm.edu.my/id/eprint/64116/1/64116.pdf
_version_	1783735400548794368

Molecular subtyping of Blastocystis sp. isolates from humans and animals in the Senoi and Proto-Malay tribes of Orang Asli, Pahang / Nabilah Amelia Mohammad

Similar Items