$The effects of tocotrienol-rich fraction on imiquimod-induced psoriatic lesion in mice / Awla Mohd Azraai$

The effects of tocotrienol-rich fraction on imiquimod-induced psoriatic lesion in mice / Awla Mohd Azraai

Background: Psoriasis is a chronic, immune-mediated, inflammatory disorder involving mainly the skin and joints. It is a global health problem which results in localised or extensive development of erythematous scaly plaques, interfering with daily activities. Psoriasis has no cure however available...

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Main Author:	Mohd Azraai, Awla
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Organic substances Vitamins Psoriasis
Online Access:	https://ir.uitm.edu.my/id/eprint/64424/1/64424.pdf
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id	my-uitm-ir.64424
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spelling	my-uitm-ir.644242022-08-08T00:47:35Z The effects of tocotrienol-rich fraction on imiquimod-induced psoriatic lesion in mice / Awla Mohd Azraai 2017-12 Mohd Azraai, Awla Organic substances Vitamins Psoriasis Background: Psoriasis is a chronic, immune-mediated, inflammatory disorder involving mainly the skin and joints. It is a global health problem which results in localised or extensive development of erythematous scaly plaques, interfering with daily activities. Psoriasis has no cure however available treatment aims at controlling symptoms despite having many side effects. Imiquimod (IMQ)-induced psoriasis murine model has been widely used in research as it mimics human psoriatic lesions at morphological, histological, immunological and genetic levels. Studies comparing the effects of IMQ on male and female mice are scarce. Tocotrienols have anti-oxidative, anti-inflammatory, anti-mitotic and anti-angiogenic properties making them a potential therapeutic candidate for psoriasis. Aim: 1) To compare between IMQ induced psoriatic lesions in male and female mice. 2) To investigate the clinical and histological effects of TRF application on the IMQ-induced psoriatic lesions. Method: Twenty-three 8-12 weeks old BALB/c mice were distributed into 4 groups; a female IMQ-TRF, male IMQ-TRF, IMQ-Placebo and IMQ control group. IMQ was applied topically to shaved backs of mice for six days. Psoriasis severity was scored every two days, using the Psoriasis Area Severity Index (PASI) including erythema, thickness and scaling. Tocotrienol-rich fraction (TRF) was applied on the skin lesions for 10 days and the PASI score recorded. The mice were sacrificed and back skin excised then examined histologically. Results: Both male and female mice developed similar clinical lesions following IMQ induction and the histological changes were comparable with features of human psoriasis. TRF improved the psoriatic lesions in male and female mice in similar days. Placebo treatment resulted in significantly less healing time compared to TRF. The histological analysis of the mice skin following TRF and placebo treatment showed little difference. Conclusion: Both male and female IMQ psoriasis mice model may be used. TRF ameliorates psoriatic lesions in mice however placebo resulted in less healing time. Red palm oil has potential therapeutic effects on psoriatic lesions. 2017-12 Thesis https://ir.uitm.edu.my/id/eprint/64424/ https://ir.uitm.edu.my/id/eprint/64424/1/64424.pdf text en public masters Universiti Teknologi MARA (Kampus Sg. Buloh) Faculty of Medicine Omar @ Abdul Rahman, Effat
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Omar @ Abdul Rahman, Effat
topic	Organic substances Vitamins Psoriasis
spellingShingle	Organic substances Vitamins Psoriasis Mohd Azraai, Awla The effects of tocotrienol-rich fraction on imiquimod-induced psoriatic lesion in mice / Awla Mohd Azraai
description	Background: Psoriasis is a chronic, immune-mediated, inflammatory disorder involving mainly the skin and joints. It is a global health problem which results in localised or extensive development of erythematous scaly plaques, interfering with daily activities. Psoriasis has no cure however available treatment aims at controlling symptoms despite having many side effects. Imiquimod (IMQ)-induced psoriasis murine model has been widely used in research as it mimics human psoriatic lesions at morphological, histological, immunological and genetic levels. Studies comparing the effects of IMQ on male and female mice are scarce. Tocotrienols have anti-oxidative, anti-inflammatory, anti-mitotic and anti-angiogenic properties making them a potential therapeutic candidate for psoriasis. Aim: 1) To compare between IMQ induced psoriatic lesions in male and female mice. 2) To investigate the clinical and histological effects of TRF application on the IMQ-induced psoriatic lesions. Method: Twenty-three 8-12 weeks old BALB/c mice were distributed into 4 groups; a female IMQ-TRF, male IMQ-TRF, IMQ-Placebo and IMQ control group. IMQ was applied topically to shaved backs of mice for six days. Psoriasis severity was scored every two days, using the Psoriasis Area Severity Index (PASI) including erythema, thickness and scaling. Tocotrienol-rich fraction (TRF) was applied on the skin lesions for 10 days and the PASI score recorded. The mice were sacrificed and back skin excised then examined histologically. Results: Both male and female mice developed similar clinical lesions following IMQ induction and the histological changes were comparable with features of human psoriasis. TRF improved the psoriatic lesions in male and female mice in similar days. Placebo treatment resulted in significantly less healing time compared to TRF. The histological analysis of the mice skin following TRF and placebo treatment showed little difference. Conclusion: Both male and female IMQ psoriasis mice model may be used. TRF ameliorates psoriatic lesions in mice however placebo resulted in less healing time. Red palm oil has potential therapeutic effects on psoriatic lesions.
format	Thesis
qualification_level	Master's degree
author	Mohd Azraai, Awla
author_facet	Mohd Azraai, Awla
author_sort	Mohd Azraai, Awla
title	The effects of tocotrienol-rich fraction on imiquimod-induced psoriatic lesion in mice / Awla Mohd Azraai
title_short	The effects of tocotrienol-rich fraction on imiquimod-induced psoriatic lesion in mice / Awla Mohd Azraai
title_full	The effects of tocotrienol-rich fraction on imiquimod-induced psoriatic lesion in mice / Awla Mohd Azraai
title_fullStr	The effects of tocotrienol-rich fraction on imiquimod-induced psoriatic lesion in mice / Awla Mohd Azraai
title_full_unstemmed	The effects of tocotrienol-rich fraction on imiquimod-induced psoriatic lesion in mice / Awla Mohd Azraai
title_sort	effects of tocotrienol-rich fraction on imiquimod-induced psoriatic lesion in mice / awla mohd azraai
granting_institution	Universiti Teknologi MARA (Kampus Sg. Buloh)
granting_department	Faculty of Medicine
publishDate	2017
url	https://ir.uitm.edu.my/id/eprint/64424/1/64424.pdf
_version_	1783735458626273280

The effects of tocotrienol-rich fraction on imiquimod-induced psoriatic lesion in mice / Awla Mohd Azraai

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