Cytotoxic activity of methanolic extract of Terminalia catappa. leaves toward MCF-7 breast cancer cell lines / Mohammad Fikkiruddin Jamsari

Terminalia catappa .L extract was popular among folk Chinese Medicine and has several proven activities as antioxidant, anticancer, antimicrobial and antinflammatory effect. In this study cytotoxic effect of two different methanolic extract of Terminalia catappa .L which are green and yellow were t...

Full description

Saved in:

Bibliographic Details
Main Author:	Jamsari, Mohammad Fikkiruddin
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Research Experimentation
Online Access:	https://ir.uitm.edu.my/id/eprint/65560/1/65560.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uitm-ir.65560
record_format	uketd_dc
spelling	my-uitm-ir.655602022-08-23T02:29:20Z Cytotoxic activity of methanolic extract of Terminalia catappa. leaves toward MCF-7 breast cancer cell lines / Mohammad Fikkiruddin Jamsari 2012 Jamsari, Mohammad Fikkiruddin Research. Experimentation Terminalia catappa .L extract was popular among folk Chinese Medicine and has several proven activities as antioxidant, anticancer, antimicrobial and antinflammatory effect. In this study cytotoxic effect of two different methanolic extract of Terminalia catappa .L which are green and yellow were tested in-vitro on positive estrogen receptor breast cancer cell line (MCF-7). By using MTT assay, MCF-7 cell line has been treated with various concentrations ranging from 10000μg/ml to lowest concentration at 24, 48 and 72 hours to evaluate the cell proliferation and viability. Methanolic extract of Terminalia catappa .L inhibited cell proliferation and reduced cell viability following the concentration and time dependent manner. Only 48 and 72 hours of treatment have shown IC50 value for both methanolic extract. Yellow methanol extract seem to be more potent than green methanol extract where the IC50 for 48 hour treatment were 1118.66 μg/ml ; 2019.57 μg/ml and 72 hours treatment were 245.54 μg/ml ; 274.46 μg/ml, respectively. Furthermore, the IC50 for each treatment had been used to assess the apoptosis feature of MCF-7 by using AO/PI staining and observed under blue light of confocal microscope. The results indicated that methanolic extract of Terminalia catappa L. have cytotoxic effect towards MCF-7 cell line. 2012 Thesis https://ir.uitm.edu.my/id/eprint/65560/ https://ir.uitm.edu.my/id/eprint/65560/1/65560.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Pharmacy Eshak, Zolkapli
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Eshak, Zolkapli
topic	Research Experimentation
spellingShingle	Research Experimentation Jamsari, Mohammad Fikkiruddin Cytotoxic activity of methanolic extract of Terminalia catappa. leaves toward MCF-7 breast cancer cell lines / Mohammad Fikkiruddin Jamsari
description	Terminalia catappa .L extract was popular among folk Chinese Medicine and has several proven activities as antioxidant, anticancer, antimicrobial and antinflammatory effect. In this study cytotoxic effect of two different methanolic extract of Terminalia catappa .L which are green and yellow were tested in-vitro on positive estrogen receptor breast cancer cell line (MCF-7). By using MTT assay, MCF-7 cell line has been treated with various concentrations ranging from 10000μg/ml to lowest concentration at 24, 48 and 72 hours to evaluate the cell proliferation and viability. Methanolic extract of Terminalia catappa .L inhibited cell proliferation and reduced cell viability following the concentration and time dependent manner. Only 48 and 72 hours of treatment have shown IC50 value for both methanolic extract. Yellow methanol extract seem to be more potent than green methanol extract where the IC50 for 48 hour treatment were 1118.66 μg/ml ; 2019.57 μg/ml and 72 hours treatment were 245.54 μg/ml ; 274.46 μg/ml, respectively. Furthermore, the IC50 for each treatment had been used to assess the apoptosis feature of MCF-7 by using AO/PI staining and observed under blue light of confocal microscope. The results indicated that methanolic extract of Terminalia catappa L. have cytotoxic effect towards MCF-7 cell line.
format	Thesis
qualification_level	Bachelor degree
author	Jamsari, Mohammad Fikkiruddin
author_facet	Jamsari, Mohammad Fikkiruddin
author_sort	Jamsari, Mohammad Fikkiruddin
title	Cytotoxic activity of methanolic extract of Terminalia catappa. leaves toward MCF-7 breast cancer cell lines / Mohammad Fikkiruddin Jamsari
title_short	Cytotoxic activity of methanolic extract of Terminalia catappa. leaves toward MCF-7 breast cancer cell lines / Mohammad Fikkiruddin Jamsari
title_full	Cytotoxic activity of methanolic extract of Terminalia catappa. leaves toward MCF-7 breast cancer cell lines / Mohammad Fikkiruddin Jamsari
title_fullStr	Cytotoxic activity of methanolic extract of Terminalia catappa. leaves toward MCF-7 breast cancer cell lines / Mohammad Fikkiruddin Jamsari
title_full_unstemmed	Cytotoxic activity of methanolic extract of Terminalia catappa. leaves toward MCF-7 breast cancer cell lines / Mohammad Fikkiruddin Jamsari
title_sort	cytotoxic activity of methanolic extract of terminalia catappa. leaves toward mcf-7 breast cancer cell lines / mohammad fikkiruddin jamsari
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Pharmacy
publishDate	2012
url	https://ir.uitm.edu.my/id/eprint/65560/1/65560.pdf
_version_	1783735559292715008

Cytotoxic activity of methanolic extract of Terminalia catappa. leaves toward MCF-7 breast cancer cell lines / Mohammad Fikkiruddin Jamsari

Similar Items