Comparison of different reverse transcriptase to generate full-length mRNA transcript by CapFinder method / Noor Haslina Zainor Abidin

Comparison of different reverse transcriptase to generate full-length mRNA transcript by CapFinder method / Noor Haslina Zainor Abidin

The CapFinder method is a method to amplifiy full-length mRNA transcripts. CapFinder technology is a much simpler method and it requires a cDNA synthesis reaction and PCR reaction to amplify the desired 5’ or 3’ ends of a cDNA. The major objective of this study is compare three different reverse tra...

Full description

Saved in:
Bibliographic Details
Main Author: Zainor Abidin, Noor Haslina
Format: Thesis
Language:English
Published: 2012
Subjects:
Genetic polymorphisms
Online Access:https://ir.uitm.edu.my/id/eprint/65565/1/65565.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-uitm-ir.65565
record_format uketd_dc
spelling my-uitm-ir.655652022-08-23T02:54:09Z Comparison of different reverse transcriptase to generate full-length mRNA transcript by CapFinder method / Noor Haslina Zainor Abidin 2012 Zainor Abidin, Noor Haslina Genetic polymorphisms The CapFinder method is a method to amplifiy full-length mRNA transcripts. CapFinder technology is a much simpler method and it requires a cDNA synthesis reaction and PCR reaction to amplify the desired 5’ or 3’ ends of a cDNA. The major objective of this study is compare three different reverse transcriptases from three different point-mutant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase which are SMARTScribe Reverse Transcriptase (Clontech), SuperScript III Reverse Transcriptase (Invitrogen) and RevertAid H Minus Reverse Transcriptase (Fermentas) to generate full-length mRNA transcript via capFinder method. The study will compare these three reverse transcriptases that vary with price range with regards to sensitivity and efficiency in generating full-length first-strand cDNA by agarose gel analysis of the amplified cDNA. 2012 Thesis https://ir.uitm.edu.my/id/eprint/65565/ https://ir.uitm.edu.my/id/eprint/65565/1/65565.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Pharmacy Mohd Yusoff, Rosmadi
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
advisor Mohd Yusoff, Rosmadi
topic Genetic polymorphisms
spellingShingle Genetic polymorphisms
Zainor Abidin, Noor Haslina
Comparison of different reverse transcriptase to generate full-length mRNA transcript by CapFinder method / Noor Haslina Zainor Abidin
description The CapFinder method is a method to amplifiy full-length mRNA transcripts. CapFinder technology is a much simpler method and it requires a cDNA synthesis reaction and PCR reaction to amplify the desired 5’ or 3’ ends of a cDNA. The major objective of this study is compare three different reverse transcriptases from three different point-mutant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase which are SMARTScribe Reverse Transcriptase (Clontech), SuperScript III Reverse Transcriptase (Invitrogen) and RevertAid H Minus Reverse Transcriptase (Fermentas) to generate full-length mRNA transcript via capFinder method. The study will compare these three reverse transcriptases that vary with price range with regards to sensitivity and efficiency in generating full-length first-strand cDNA by agarose gel analysis of the amplified cDNA.
format Thesis
qualification_level Bachelor degree
author Zainor Abidin, Noor Haslina
author_facet Zainor Abidin, Noor Haslina
author_sort Zainor Abidin, Noor Haslina
title Comparison of different reverse transcriptase to generate full-length mRNA transcript by CapFinder method / Noor Haslina Zainor Abidin
title_short Comparison of different reverse transcriptase to generate full-length mRNA transcript by CapFinder method / Noor Haslina Zainor Abidin
title_full Comparison of different reverse transcriptase to generate full-length mRNA transcript by CapFinder method / Noor Haslina Zainor Abidin
title_fullStr Comparison of different reverse transcriptase to generate full-length mRNA transcript by CapFinder method / Noor Haslina Zainor Abidin
title_full_unstemmed Comparison of different reverse transcriptase to generate full-length mRNA transcript by CapFinder method / Noor Haslina Zainor Abidin
title_sort comparison of different reverse transcriptase to generate full-length mrna transcript by capfinder method / noor haslina zainor abidin
granting_institution Universiti Teknologi MARA (UiTM)
granting_department Faculty of Pharmacy
publishDate 2012
url https://ir.uitm.edu.my/id/eprint/65565/1/65565.pdf
_version_ 1783735560323465216

Similar Items