Mechanism of apoptosis on pure polyphenols: analysis of flow cytometry / Nurul Zuhanis Mohamed

The incidence of hepatocellular carcinoma is increasing in Malaysia and several new cases are being diagnosed every year. The purpose of this research was to find out the mechanism of apoptosis on pure polyphenols ; curcumin and gallic acid on HepG2 (hepatocellular carcinoma) cell line by using flow...

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Main Author:	Mohamed, Nurul Zuhanis
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Research Experimentation
Online Access:	https://ir.uitm.edu.my/id/eprint/65620/1/65620.pdf
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id	my-uitm-ir.65620
record_format	uketd_dc
spelling	my-uitm-ir.656202022-11-07T07:52:52Z Mechanism of apoptosis on pure polyphenols: analysis of flow cytometry / Nurul Zuhanis Mohamed 2012 Mohamed, Nurul Zuhanis Research. Experimentation The incidence of hepatocellular carcinoma is increasing in Malaysia and several new cases are being diagnosed every year. The purpose of this research was to find out the mechanism of apoptosis on pure polyphenols ; curcumin and gallic acid on HepG2 (hepatocellular carcinoma) cell line by using flow cytometry. HepG2 cell line was exposed to different concentrations of gallic acid and curcumin (IC25, IC50 and IC75) in 24 and 48 hours. The outer membrane protrusion of phosphatidylserine which is typically inaccessible in viable cells, is detected by annexin V-FITC, and indicates the early stages of apoptosis. From the results, these polyphenolic compounds significantly induced apoptosis in HepG2 cells. Result also revealed that gallic acid have the ability to induce early-apoptotic cell death (P<0.05) in HepG2 cells at lower concentration than curcumin. In conclusion, polyphenols which are curcumin and gallic acid are able to induce apoptosis on HepG2 cell line. This may be a beginning in the search for a new anticancer agent. 2012 Thesis https://ir.uitm.edu.my/id/eprint/65620/ https://ir.uitm.edu.my/id/eprint/65620/1/65620.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Pharmacy Hazizul Hasan, Mizaton
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Hazizul Hasan, Mizaton
topic	Research Experimentation
spellingShingle	Research Experimentation Mohamed, Nurul Zuhanis Mechanism of apoptosis on pure polyphenols: analysis of flow cytometry / Nurul Zuhanis Mohamed
description	The incidence of hepatocellular carcinoma is increasing in Malaysia and several new cases are being diagnosed every year. The purpose of this research was to find out the mechanism of apoptosis on pure polyphenols ; curcumin and gallic acid on HepG2 (hepatocellular carcinoma) cell line by using flow cytometry. HepG2 cell line was exposed to different concentrations of gallic acid and curcumin (IC25, IC50 and IC75) in 24 and 48 hours. The outer membrane protrusion of phosphatidylserine which is typically inaccessible in viable cells, is detected by annexin V-FITC, and indicates the early stages of apoptosis. From the results, these polyphenolic compounds significantly induced apoptosis in HepG2 cells. Result also revealed that gallic acid have the ability to induce early-apoptotic cell death (P<0.05) in HepG2 cells at lower concentration than curcumin. In conclusion, polyphenols which are curcumin and gallic acid are able to induce apoptosis on HepG2 cell line. This may be a beginning in the search for a new anticancer agent.
format	Thesis
qualification_level	Bachelor degree
author	Mohamed, Nurul Zuhanis
author_facet	Mohamed, Nurul Zuhanis
author_sort	Mohamed, Nurul Zuhanis
title	Mechanism of apoptosis on pure polyphenols: analysis of flow cytometry / Nurul Zuhanis Mohamed
title_short	Mechanism of apoptosis on pure polyphenols: analysis of flow cytometry / Nurul Zuhanis Mohamed
title_full	Mechanism of apoptosis on pure polyphenols: analysis of flow cytometry / Nurul Zuhanis Mohamed
title_fullStr	Mechanism of apoptosis on pure polyphenols: analysis of flow cytometry / Nurul Zuhanis Mohamed
title_full_unstemmed	Mechanism of apoptosis on pure polyphenols: analysis of flow cytometry / Nurul Zuhanis Mohamed
title_sort	mechanism of apoptosis on pure polyphenols: analysis of flow cytometry / nurul zuhanis mohamed
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Pharmacy
publishDate	2012
url	https://ir.uitm.edu.my/id/eprint/65620/1/65620.pdf
_version_	1783735562588389376

Mechanism of apoptosis on pure polyphenols: analysis of flow cytometry / Nurul Zuhanis Mohamed

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