In vitro free radical formation following exposure to Technetium-99M (99mTc) / Ahmad Akmal Mohamad Bee

This study is about the formation of free radical following exposure to technetium-99m (99mTc). The objectives are to assess the presence of free radical following administration of 99mTc-HMPAO and also to assess the optimum time and activity to induce the free radical formation. This 99mTc will ir...

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Main Author:	Mohamad Bee, Ahmad Akmal
Format:	Thesis
Language:	English
Published:	2012
Subjects:	DNA Deoxyribonucleic acids Analytical chemistry
Online Access:	https://ir.uitm.edu.my/id/eprint/66674/1/66674.pdf
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id	my-uitm-ir.66674
record_format	uketd_dc
spelling	my-uitm-ir.666742022-09-14T03:25:30Z In vitro free radical formation following exposure to Technetium-99M (99mTc) / Ahmad Akmal Mohamad Bee 2012 Mohamad Bee, Ahmad Akmal DNA. Deoxyribonucleic acids Analytical chemistry This study is about the formation of free radical following exposure to technetium-99m (99mTc). The objectives are to assess the presence of free radical following administration of 99mTc-HMPAO and also to assess the optimum time and activity to induce the free radical formation. This 99mTc will irradiate and produce the free radical known as auger electrons. The method that used in this study is as follow. The 99mTc is produced by the 99mTc generator. Then it must undergo the radionuclide purity test to measure the purity of 99mTc by the generator. After that, the 99mTc is form a complex with the Hexamethylpropyleneamine oxime (HMPAO). The amount of complex that formed was measured to get the purity standard. Then, the complex will be labelled into the cell and the percentage uptake of complex by cell will be recorded. The last stage of this experiment is the thiobarbituric acid reactive substance. This test is done to detect the presence of free radical that form in the cell by using the malondialdehyde (MDA) standard curve. 2012 Thesis https://ir.uitm.edu.my/id/eprint/66674/ https://ir.uitm.edu.my/id/eprint/66674/1/66674.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Pharmacy Othman, Muhamad Faiz
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Othman, Muhamad Faiz
topic	DNA Deoxyribonucleic acids Analytical chemistry
spellingShingle	DNA Deoxyribonucleic acids Analytical chemistry Mohamad Bee, Ahmad Akmal In vitro free radical formation following exposure to Technetium-99M (99mTc) / Ahmad Akmal Mohamad Bee
description	This study is about the formation of free radical following exposure to technetium-99m (99mTc). The objectives are to assess the presence of free radical following administration of 99mTc-HMPAO and also to assess the optimum time and activity to induce the free radical formation. This 99mTc will irradiate and produce the free radical known as auger electrons. The method that used in this study is as follow. The 99mTc is produced by the 99mTc generator. Then it must undergo the radionuclide purity test to measure the purity of 99mTc by the generator. After that, the 99mTc is form a complex with the Hexamethylpropyleneamine oxime (HMPAO). The amount of complex that formed was measured to get the purity standard. Then, the complex will be labelled into the cell and the percentage uptake of complex by cell will be recorded. The last stage of this experiment is the thiobarbituric acid reactive substance. This test is done to detect the presence of free radical that form in the cell by using the malondialdehyde (MDA) standard curve.
format	Thesis
qualification_level	Bachelor degree
author	Mohamad Bee, Ahmad Akmal
author_facet	Mohamad Bee, Ahmad Akmal
author_sort	Mohamad Bee, Ahmad Akmal
title	In vitro free radical formation following exposure to Technetium-99M (99mTc) / Ahmad Akmal Mohamad Bee
title_short	In vitro free radical formation following exposure to Technetium-99M (99mTc) / Ahmad Akmal Mohamad Bee
title_full	In vitro free radical formation following exposure to Technetium-99M (99mTc) / Ahmad Akmal Mohamad Bee
title_fullStr	In vitro free radical formation following exposure to Technetium-99M (99mTc) / Ahmad Akmal Mohamad Bee
title_full_unstemmed	In vitro free radical formation following exposure to Technetium-99M (99mTc) / Ahmad Akmal Mohamad Bee
title_sort	in vitro free radical formation following exposure to technetium-99m (99mtc) / ahmad akmal mohamad bee
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Pharmacy
publishDate	2012
url	https://ir.uitm.edu.my/id/eprint/66674/1/66674.pdf
_version_	1783735629493829632

In vitro free radical formation following exposure to Technetium-99M (99mTc) / Ahmad Akmal Mohamad Bee

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