Isolation of bioactive fungal metabolites from endophytic fungi / Nasreen Nazmi

The study was done to investigate the secondary metabolites produced by endophytic fungi of 3PR3 that can be used for medicinal plant purpose. The study was done by growing the fungi on Potato Dextrose Agar Plate (PDA). Then the cultures were incubated for a two-week period. After that, extraction o...

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Main Author:	Nazmi, Nasreen
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Antibacterial agents Medicinal plants (Culture only)
Online Access:	https://ir.uitm.edu.my/id/eprint/66704/1/66704.pdf
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id	my-uitm-ir.66704
record_format	uketd_dc
spelling	my-uitm-ir.667042022-09-14T03:15:19Z Isolation of bioactive fungal metabolites from endophytic fungi / Nasreen Nazmi 2012 Nazmi, Nasreen Antibacterial agents Medicinal plants (Culture only) The study was done to investigate the secondary metabolites produced by endophytic fungi of 3PR3 that can be used for medicinal plant purpose. The study was done by growing the fungi on Potato Dextrose Agar Plate (PDA). Then the cultures were incubated for a two-week period. After that, extraction of the fungi metabolite was performed by using ethyl acetate as a solvent. Fungal extracts had been subjected to High performance liquid chromatography (HPLC) using a diode array detector (DAD) to see the chromatogram and compare between batches. Then proceed to Semi PREP HPLC and collected all peaks in vial. After that fractions had been biologically tested on antibacterial activity and cytotoxic activity. From the observation, antibacterial test by MTT assay show negative result on all plate means there is no antibacterial activity produced by the extract of fungi 3PR3 against bacteria Pseudomonas Aeroginosa. 2012 Thesis https://ir.uitm.edu.my/id/eprint/66704/ https://ir.uitm.edu.my/id/eprint/66704/1/66704.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Pharmacy Sultan, Sadia Weber Abdullah, J.F.F. Wan Iryani Ramasamy, Kalavathy Syed Adnan
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Sultan, Sadia Weber Abdullah, J.F.F. Wan Iryani Ramasamy, Kalavathy Syed Adnan
topic	Antibacterial agents Medicinal plants (Culture only)
spellingShingle	Antibacterial agents Medicinal plants (Culture only) Nazmi, Nasreen Isolation of bioactive fungal metabolites from endophytic fungi / Nasreen Nazmi
description	The study was done to investigate the secondary metabolites produced by endophytic fungi of 3PR3 that can be used for medicinal plant purpose. The study was done by growing the fungi on Potato Dextrose Agar Plate (PDA). Then the cultures were incubated for a two-week period. After that, extraction of the fungi metabolite was performed by using ethyl acetate as a solvent. Fungal extracts had been subjected to High performance liquid chromatography (HPLC) using a diode array detector (DAD) to see the chromatogram and compare between batches. Then proceed to Semi PREP HPLC and collected all peaks in vial. After that fractions had been biologically tested on antibacterial activity and cytotoxic activity. From the observation, antibacterial test by MTT assay show negative result on all plate means there is no antibacterial activity produced by the extract of fungi 3PR3 against bacteria Pseudomonas Aeroginosa.
format	Thesis
qualification_level	Bachelor degree
author	Nazmi, Nasreen
author_facet	Nazmi, Nasreen
author_sort	Nazmi, Nasreen
title	Isolation of bioactive fungal metabolites from endophytic fungi / Nasreen Nazmi
title_short	Isolation of bioactive fungal metabolites from endophytic fungi / Nasreen Nazmi
title_full	Isolation of bioactive fungal metabolites from endophytic fungi / Nasreen Nazmi
title_fullStr	Isolation of bioactive fungal metabolites from endophytic fungi / Nasreen Nazmi
title_full_unstemmed	Isolation of bioactive fungal metabolites from endophytic fungi / Nasreen Nazmi
title_sort	isolation of bioactive fungal metabolites from endophytic fungi / nasreen nazmi
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Pharmacy
publishDate	2012
url	https://ir.uitm.edu.my/id/eprint/66704/1/66704.pdf
_version_	1783735634164187136

Isolation of bioactive fungal metabolites from endophytic fungi / Nasreen Nazmi

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