Cytotoxic activity of myricetin and mahanimbine against human glioblastoma cell line (SF268) and normal cell line (WRL 68) / Norhamiza Abd Hamid

Nature has been a source of medicinal agents for thousands of years, particularly plants have a long history of use in the treatment of cancer. The study aims to evaluate cytotoxicity of compounds from plants which are mahanimbine from Murraya koenigii and myricetin as a flavonoid against glioblasto...

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Main Author:	Abd Hamid, Norhamiza
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Cytology Cytotoxic drugs
Online Access:	https://ir.uitm.edu.my/id/eprint/66743/1/66743.pdf
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id	my-uitm-ir.66743
record_format	uketd_dc
spelling	my-uitm-ir.667432022-09-15T00:28:21Z Cytotoxic activity of myricetin and mahanimbine against human glioblastoma cell line (SF268) and normal cell line (WRL 68) / Norhamiza Abd Hamid 2012 Abd Hamid, Norhamiza Cytology Cytotoxic drugs Nature has been a source of medicinal agents for thousands of years, particularly plants have a long history of use in the treatment of cancer. The study aims to evaluate cytotoxicity of compounds from plants which are mahanimbine from Murraya koenigii and myricetin as a flavonoid against glioblastoma cell line SF268 and also identified their selectivity towards normal cell line WRL68. Both cells were cultured in MEM supplemented with 10% (v/v) deactivated fetal bovine serum and 1% penicillin/streptomycin at 37oC with 5% CO2. The compounds (50μg/mL to 0.005μg/mL) were screened for their cytotoxicity activity against both cell lines, SF268 and WRL68. After 72hours incubation, the inhibitory concentration to afford 50% cell viability (IC50) for these compounds was determined by MTT assays and the data generated from ELISA plate reader were used to plot the dose response curve. The myricetin exhibited an IC50 of 3μg/mL and mahanimbine has an IC50 of 9.5μg/mL against SF268 cell lines. Nevertheless, these compounds also showed cytotoxic towards normal cell line WRL68. From this study showed both compounds have potential as anticancer agent but need further research to improve the selectivity of the compounds towards cancerous cells rather than damaging the normal cells. 2012 Thesis https://ir.uitm.edu.my/id/eprint/66743/ https://ir.uitm.edu.my/id/eprint/66743/1/66743.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Pharmacy Mani, Vasudevan Ramasamy, Kalavathy Mohd Nor Hazamin, Nurul Aqmar
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Mani, Vasudevan Ramasamy, Kalavathy Mohd Nor Hazamin, Nurul Aqmar
topic	Cytology Cytotoxic drugs
spellingShingle	Cytology Cytotoxic drugs Abd Hamid, Norhamiza Cytotoxic activity of myricetin and mahanimbine against human glioblastoma cell line (SF268) and normal cell line (WRL 68) / Norhamiza Abd Hamid
description	Nature has been a source of medicinal agents for thousands of years, particularly plants have a long history of use in the treatment of cancer. The study aims to evaluate cytotoxicity of compounds from plants which are mahanimbine from Murraya koenigii and myricetin as a flavonoid against glioblastoma cell line SF268 and also identified their selectivity towards normal cell line WRL68. Both cells were cultured in MEM supplemented with 10% (v/v) deactivated fetal bovine serum and 1% penicillin/streptomycin at 37oC with 5% CO2. The compounds (50μg/mL to 0.005μg/mL) were screened for their cytotoxicity activity against both cell lines, SF268 and WRL68. After 72hours incubation, the inhibitory concentration to afford 50% cell viability (IC50) for these compounds was determined by MTT assays and the data generated from ELISA plate reader were used to plot the dose response curve. The myricetin exhibited an IC50 of 3μg/mL and mahanimbine has an IC50 of 9.5μg/mL against SF268 cell lines. Nevertheless, these compounds also showed cytotoxic towards normal cell line WRL68. From this study showed both compounds have potential as anticancer agent but need further research to improve the selectivity of the compounds towards cancerous cells rather than damaging the normal cells.
format	Thesis
qualification_level	Bachelor degree
author	Abd Hamid, Norhamiza
author_facet	Abd Hamid, Norhamiza
author_sort	Abd Hamid, Norhamiza
title	Cytotoxic activity of myricetin and mahanimbine against human glioblastoma cell line (SF268) and normal cell line (WRL 68) / Norhamiza Abd Hamid
title_short	Cytotoxic activity of myricetin and mahanimbine against human glioblastoma cell line (SF268) and normal cell line (WRL 68) / Norhamiza Abd Hamid
title_full	Cytotoxic activity of myricetin and mahanimbine against human glioblastoma cell line (SF268) and normal cell line (WRL 68) / Norhamiza Abd Hamid
title_fullStr	Cytotoxic activity of myricetin and mahanimbine against human glioblastoma cell line (SF268) and normal cell line (WRL 68) / Norhamiza Abd Hamid
title_full_unstemmed	Cytotoxic activity of myricetin and mahanimbine against human glioblastoma cell line (SF268) and normal cell line (WRL 68) / Norhamiza Abd Hamid
title_sort	cytotoxic activity of myricetin and mahanimbine against human glioblastoma cell line (sf268) and normal cell line (wrl 68) / norhamiza abd hamid
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Pharmacy
publishDate	2012
url	https://ir.uitm.edu.my/id/eprint/66743/1/66743.pdf
_version_	1783735636544454656

Cytotoxic activity of myricetin and mahanimbine against human glioblastoma cell line (SF268) and normal cell line (WRL 68) / Norhamiza Abd Hamid

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