In vitro antiproliferative activity of the methanol extract of Bauhinia purpurea leaves / Nurfatihah Nasiha Bahagia

Background: Nowadays, there is an increasing burden of cancer in developed countries. The most common cancers in the world are liver cancer, breast cancer and colon cancer. Previous finding and latest studies show that Bauhinia purpurea or in Malay known as 'pokok tapak kerbau' have potent...

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Main Author:	Bahagia, Nurfatihah Nasiha
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Individual drugs and other agents
Online Access:	https://ir.uitm.edu.my/id/eprint/66796/1/66796.pdf
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id	my-uitm-ir.66796
record_format	uketd_dc
spelling	my-uitm-ir.667962022-09-15T05:03:24Z In vitro antiproliferative activity of the methanol extract of Bauhinia purpurea leaves / Nurfatihah Nasiha Bahagia 2012 Bahagia, Nurfatihah Nasiha Individual drugs and other agents Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic) Background: Nowadays, there is an increasing burden of cancer in developed countries. The most common cancers in the world are liver cancer, breast cancer and colon cancer. Previous finding and latest studies show that Bauhinia purpurea or in Malay known as 'pokok tapak kerbau' have potential inhibition property to human cancer cells lines due to the new compounds which are named Bauhiniastatin 1, 2, 3, and pacharin. The purpose of this study is to evaluate antiproliferative activities of Bauhinia purpurea leaves methanolic extract on cancer cells lines and not affect normal cells lines. This study used four types of cell lines consists of Hep G2 (human hepatocarcinoma cells lines) MCF-7 (human breast adenocarcinoma cell line), HCT 116 (colorectal carcinoma cells lines) and WRL 68 (normal cells lines). Methods: Cells were plated in 96-well plate and incubated in conditions of 95% O2, 5% CO2 at 37°C. Five different concentrations of Bauhinia purpurea leaves methanolic extract were tested on cells lines; 0.1, 1, 10, 100, 1000 ug/mL and 3-[4,5-dimethylthioazol-2-yl]-2-5-diphenyltetrazolium bromide (MTT) assay was used to measure cell viability. The effects of different concentrations on percentage of cell viability and calculation of median inhibitory concentration (IC50) were determined by plotting graph percentage of cell viability versus log concentration of Bauhinia purpurea leaves methanolic extract. Results: Bauhinia purpurea leaves methanolic extract has potential antiproliferative activity against Hep G2 cancer cells lines, MCF-7 cancer cells lines, HCT 116 cancer cells lines by producing median inhibitory concentration IC50 of 143.3 ug/mL, 167.7 ug/mL, 104.2 ug/mL respectively. Conclusion: Methanolic extract of Bauhinia purpurea leaves exhibit potential anticancer activity and can be used as preliminary study to do further research on antiproliferative activity of Bauhinia purpurea leaves. 2012 Thesis https://ir.uitm.edu.my/id/eprint/66796/ https://ir.uitm.edu.my/id/eprint/66796/1/66796.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Pharmacy Yob, Noor Jannah
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Yob, Noor Jannah
topic	Individual drugs and other agents Individual drugs and other agents
spellingShingle	Individual drugs and other agents Individual drugs and other agents Bahagia, Nurfatihah Nasiha In vitro antiproliferative activity of the methanol extract of Bauhinia purpurea leaves / Nurfatihah Nasiha Bahagia
description	Background: Nowadays, there is an increasing burden of cancer in developed countries. The most common cancers in the world are liver cancer, breast cancer and colon cancer. Previous finding and latest studies show that Bauhinia purpurea or in Malay known as 'pokok tapak kerbau' have potential inhibition property to human cancer cells lines due to the new compounds which are named Bauhiniastatin 1, 2, 3, and pacharin. The purpose of this study is to evaluate antiproliferative activities of Bauhinia purpurea leaves methanolic extract on cancer cells lines and not affect normal cells lines. This study used four types of cell lines consists of Hep G2 (human hepatocarcinoma cells lines) MCF-7 (human breast adenocarcinoma cell line), HCT 116 (colorectal carcinoma cells lines) and WRL 68 (normal cells lines). Methods: Cells were plated in 96-well plate and incubated in conditions of 95% O2, 5% CO2 at 37°C. Five different concentrations of Bauhinia purpurea leaves methanolic extract were tested on cells lines; 0.1, 1, 10, 100, 1000 ug/mL and 3-[4,5-dimethylthioazol-2-yl]-2-5-diphenyltetrazolium bromide (MTT) assay was used to measure cell viability. The effects of different concentrations on percentage of cell viability and calculation of median inhibitory concentration (IC50) were determined by plotting graph percentage of cell viability versus log concentration of Bauhinia purpurea leaves methanolic extract. Results: Bauhinia purpurea leaves methanolic extract has potential antiproliferative activity against Hep G2 cancer cells lines, MCF-7 cancer cells lines, HCT 116 cancer cells lines by producing median inhibitory concentration IC50 of 143.3 ug/mL, 167.7 ug/mL, 104.2 ug/mL respectively. Conclusion: Methanolic extract of Bauhinia purpurea leaves exhibit potential anticancer activity and can be used as preliminary study to do further research on antiproliferative activity of Bauhinia purpurea leaves.
format	Thesis
qualification_level	Bachelor degree
author	Bahagia, Nurfatihah Nasiha
author_facet	Bahagia, Nurfatihah Nasiha
author_sort	Bahagia, Nurfatihah Nasiha
title	In vitro antiproliferative activity of the methanol extract of Bauhinia purpurea leaves / Nurfatihah Nasiha Bahagia
title_short	In vitro antiproliferative activity of the methanol extract of Bauhinia purpurea leaves / Nurfatihah Nasiha Bahagia
title_full	In vitro antiproliferative activity of the methanol extract of Bauhinia purpurea leaves / Nurfatihah Nasiha Bahagia
title_fullStr	In vitro antiproliferative activity of the methanol extract of Bauhinia purpurea leaves / Nurfatihah Nasiha Bahagia
title_full_unstemmed	In vitro antiproliferative activity of the methanol extract of Bauhinia purpurea leaves / Nurfatihah Nasiha Bahagia
title_sort	in vitro antiproliferative activity of the methanol extract of bauhinia purpurea leaves / nurfatihah nasiha bahagia
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Pharmacy
publishDate	2012
url	https://ir.uitm.edu.my/id/eprint/66796/1/66796.pdf
_version_	1783735639512973312

In vitro antiproliferative activity of the methanol extract of Bauhinia purpurea leaves / Nurfatihah Nasiha Bahagia

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