Cytotoxicity effects of Melastoma malabathricum leaves methanolic extract on cell viability / Wan Norshahadah Wan Shamsuddin

Melastoma malabathricum has a wide distribution around this part of the world. It has been reported to be found growing wild in Southeast Asia including Malaysia. The present study aims to determine the anticancer activities of methanolic extracts from the leaves of Melastoma malabathricum using var...

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Main Author:	Wan Shamsuddin, Wan Norshahadah
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Botanical chemistry Phytochemicals Cancer
Online Access:	https://ir.uitm.edu.my/id/eprint/66822/1/66822.pdf
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id	my-uitm-ir.66822
record_format	uketd_dc
spelling	my-uitm-ir.668222022-09-15T08:16:14Z Cytotoxicity effects of Melastoma malabathricum leaves methanolic extract on cell viability / Wan Norshahadah Wan Shamsuddin 2012 Wan Shamsuddin, Wan Norshahadah Botanical chemistry. Phytochemicals Cancer Herbs. Herbal teas Melastoma malabathricum has a wide distribution around this part of the world. It has been reported to be found growing wild in Southeast Asia including Malaysia. The present study aims to determine the anticancer activities of methanolic extracts from the leaves of Melastoma malabathricum using various established in vitro assays. Four types of cell lines were utilized in this study which were breast cancer cells (MCF-7), hepatocellular carcinoma cells (HepG2), colon cancer cells (HCT 116) and normal liver cells (WRL 68). Cells were plated in 96-well plates and incubated in conditions at 370C under 95% O2 and 5% CO2. Five concentrations of M. malabathricum extracts; 0.1 μg/ml, 1 μg/ml, 10 μg/ml, 100 μg/ml, 1000 μg/ml were selected to verify the anticancer activities and MTT assay was chosen as the method to measure the cell viability. Concentration response curve for methanol extract of M. malabathricum were constructed to determine the effects of concentration on cell viability and the median inhibitory concentration (IC50) was calculated for each cell line. IC50 for methanolic extract of M. malabathricum in MCF-7 cells, HepG2 cells, HCT 116 cells and WRL 68 cells were 126.9 ± 19.9μg/ml, 130.6 ± 29.3μg/ml, 124.9 ± 44.1μg/ml and 261.4 ± 17.7μg/ml respectively. . From the result, MCF-7, HepG2 and HCT 116 cells showed greater cytotoxic activity compared to WRL 68 cells as IC50, the concentration of the extract that provides 50% inhibition to the cells is lower compared to WRL 68. This study can contribute to the development of more effective, safer and cheaper natural based anticancer drugs. 2012 Thesis https://ir.uitm.edu.my/id/eprint/66822/ https://ir.uitm.edu.my/id/eprint/66822/1/66822.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Pharmacy Noor Jannah
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Noor Jannah
topic	Botanical chemistry Phytochemicals Cancer Botanical chemistry Phytochemicals
spellingShingle	Botanical chemistry Phytochemicals Cancer Botanical chemistry Phytochemicals Wan Shamsuddin, Wan Norshahadah Cytotoxicity effects of Melastoma malabathricum leaves methanolic extract on cell viability / Wan Norshahadah Wan Shamsuddin
description	Melastoma malabathricum has a wide distribution around this part of the world. It has been reported to be found growing wild in Southeast Asia including Malaysia. The present study aims to determine the anticancer activities of methanolic extracts from the leaves of Melastoma malabathricum using various established in vitro assays. Four types of cell lines were utilized in this study which were breast cancer cells (MCF-7), hepatocellular carcinoma cells (HepG2), colon cancer cells (HCT 116) and normal liver cells (WRL 68). Cells were plated in 96-well plates and incubated in conditions at 370C under 95% O2 and 5% CO2. Five concentrations of M. malabathricum extracts; 0.1 μg/ml, 1 μg/ml, 10 μg/ml, 100 μg/ml, 1000 μg/ml were selected to verify the anticancer activities and MTT assay was chosen as the method to measure the cell viability. Concentration response curve for methanol extract of M. malabathricum were constructed to determine the effects of concentration on cell viability and the median inhibitory concentration (IC50) was calculated for each cell line. IC50 for methanolic extract of M. malabathricum in MCF-7 cells, HepG2 cells, HCT 116 cells and WRL 68 cells were 126.9 ± 19.9μg/ml, 130.6 ± 29.3μg/ml, 124.9 ± 44.1μg/ml and 261.4 ± 17.7μg/ml respectively. . From the result, MCF-7, HepG2 and HCT 116 cells showed greater cytotoxic activity compared to WRL 68 cells as IC50, the concentration of the extract that provides 50% inhibition to the cells is lower compared to WRL 68. This study can contribute to the development of more effective, safer and cheaper natural based anticancer drugs.
format	Thesis
qualification_level	Bachelor degree
author	Wan Shamsuddin, Wan Norshahadah
author_facet	Wan Shamsuddin, Wan Norshahadah
author_sort	Wan Shamsuddin, Wan Norshahadah
title	Cytotoxicity effects of Melastoma malabathricum leaves methanolic extract on cell viability / Wan Norshahadah Wan Shamsuddin
title_short	Cytotoxicity effects of Melastoma malabathricum leaves methanolic extract on cell viability / Wan Norshahadah Wan Shamsuddin
title_full	Cytotoxicity effects of Melastoma malabathricum leaves methanolic extract on cell viability / Wan Norshahadah Wan Shamsuddin
title_fullStr	Cytotoxicity effects of Melastoma malabathricum leaves methanolic extract on cell viability / Wan Norshahadah Wan Shamsuddin
title_full_unstemmed	Cytotoxicity effects of Melastoma malabathricum leaves methanolic extract on cell viability / Wan Norshahadah Wan Shamsuddin
title_sort	cytotoxicity effects of melastoma malabathricum leaves methanolic extract on cell viability / wan norshahadah wan shamsuddin
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Pharmacy
publishDate	2012
url	https://ir.uitm.edu.my/id/eprint/66822/1/66822.pdf
_version_	1783735643306721280

Cytotoxicity effects of Melastoma malabathricum leaves methanolic extract on cell viability / Wan Norshahadah Wan Shamsuddin

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