Screening and identification of thermophilic microorganisms that produce extracellular lipase from Hulu Langat Hot Spring / Ummul Hanan Mohamad

Lipolytic thermophilic bacteria was successfully isolated from Hulu Langat hot spring water. Molecular biological technique based on 16SRNA analysis revealed that the isolated bacteria species was Bacillus subtilis. Selective isolation of bacteria from hotspring water is performed using Rhodamine-B...

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Main Author:	Mohamad, Ummul Hanan
Format:	Thesis
Language:	English
Published:	2009
Subjects:	Environmental protection
Online Access:	https://ir.uitm.edu.my/id/eprint/70121/1/70121.pdf
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id	my-uitm-ir.70121
record_format	uketd_dc
spelling	my-uitm-ir.701212023-05-21T14:47:32Z Screening and identification of thermophilic microorganisms that produce extracellular lipase from Hulu Langat Hot Spring / Ummul Hanan Mohamad 2009 Mohamad, Ummul Hanan Environmental protection Lipolytic thermophilic bacteria was successfully isolated from Hulu Langat hot spring water. Molecular biological technique based on 16SRNA analysis revealed that the isolated bacteria species was Bacillus subtilis. Selective isolation of bacteria from hotspring water is performed using Rhodamine-B Tween 80 agar in which lipase producer is identified based on presence of orange fluorescence halos around colonies when plate is irradiated with UV light. Further characterisation of isolated bacteria is performed based on the colony characteristics on media as well as Gram staining. Bacillus subtilis isolated from hotspring appeared as clear looking colony with reddish tint. It was irregular in shaped, flat, mucoid and big. It was also motile and catalase positive. Under Gram staining, it was arranged in chains and was big long rods. The isolated lipase producer grew best at 55°C at alkaline pH of 8.1. 2009 Thesis https://ir.uitm.edu.my/id/eprint/70121/ https://ir.uitm.edu.my/id/eprint/70121/1/70121.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Lee, Hung Kiong
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Lee, Hung Kiong
topic	Environmental protection
spellingShingle	Environmental protection Mohamad, Ummul Hanan Screening and identification of thermophilic microorganisms that produce extracellular lipase from Hulu Langat Hot Spring / Ummul Hanan Mohamad
description	Lipolytic thermophilic bacteria was successfully isolated from Hulu Langat hot spring water. Molecular biological technique based on 16SRNA analysis revealed that the isolated bacteria species was Bacillus subtilis. Selective isolation of bacteria from hotspring water is performed using Rhodamine-B Tween 80 agar in which lipase producer is identified based on presence of orange fluorescence halos around colonies when plate is irradiated with UV light. Further characterisation of isolated bacteria is performed based on the colony characteristics on media as well as Gram staining. Bacillus subtilis isolated from hotspring appeared as clear looking colony with reddish tint. It was irregular in shaped, flat, mucoid and big. It was also motile and catalase positive. Under Gram staining, it was arranged in chains and was big long rods. The isolated lipase producer grew best at 55°C at alkaline pH of 8.1.
format	Thesis
qualification_level	Bachelor degree
author	Mohamad, Ummul Hanan
author_facet	Mohamad, Ummul Hanan
author_sort	Mohamad, Ummul Hanan
title	Screening and identification of thermophilic microorganisms that produce extracellular lipase from Hulu Langat Hot Spring / Ummul Hanan Mohamad
title_short	Screening and identification of thermophilic microorganisms that produce extracellular lipase from Hulu Langat Hot Spring / Ummul Hanan Mohamad
title_full	Screening and identification of thermophilic microorganisms that produce extracellular lipase from Hulu Langat Hot Spring / Ummul Hanan Mohamad
title_fullStr	Screening and identification of thermophilic microorganisms that produce extracellular lipase from Hulu Langat Hot Spring / Ummul Hanan Mohamad
title_full_unstemmed	Screening and identification of thermophilic microorganisms that produce extracellular lipase from Hulu Langat Hot Spring / Ummul Hanan Mohamad
title_sort	screening and identification of thermophilic microorganisms that produce extracellular lipase from hulu langat hot spring / ummul hanan mohamad
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Sciences
publishDate	2009
url	https://ir.uitm.edu.my/id/eprint/70121/1/70121.pdf
_version_	1783735924990935040

Screening and identification of thermophilic microorganisms that produce extracellular lipase from Hulu Langat Hot Spring / Ummul Hanan Mohamad

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