FCGR3B copy number variation and host susceptibility to vascular leakage in dengue hemorrhagic fever / Umairah Mokhtar

Dengue is a serious public health threat in many countries around the world. It is estimated that 50- 100 million cases of severe dengue requiring hospitalization occurs annually. Approximately 500,000 of these cases manifested as DHF/ Dengue Shock Syndrome (DSS) with more than 20, 000 deaths worldw...

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Main Author:	Mokhtar, Umairah
Format:	Thesis
Language:	English
Published:	2011
Subjects:	Dengue
Online Access:	https://ir.uitm.edu.my/id/eprint/72170/1/72170.pdf
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id	my-uitm-ir.72170
record_format	uketd_dc
spelling	my-uitm-ir.721702024-01-02T08:45:51Z FCGR3B copy number variation and host susceptibility to vascular leakage in dengue hemorrhagic fever / Umairah Mokhtar 2011 Mokhtar, Umairah Dengue Dengue is a serious public health threat in many countries around the world. It is estimated that 50- 100 million cases of severe dengue requiring hospitalization occurs annually. Approximately 500,000 of these cases manifested as DHF/ Dengue Shock Syndrome (DSS) with more than 20, 000 deaths worldwide. Dengue deaths occur usually as a complication of severe vascular leakage. The mechanisms that lead to severe vascular leakage are still unknown. Recent studies suggested the potential role of Fe gamma receptors (FCGRs) in the pathogenesis of dengue. In the present study, copy number variation of the FCGR3B genes in a select dengue patient population in Malaysia is estimated. Methodology: Total cell DNA was extracted from dengue patients' whole blood. CNV genotyping was . performed using the real time quantitative PCR (qPCR) with SYBR Green fluorescent dye assay (Bio Rad). In qPCR analysis, the relative quantification was used to analyse relative changes in CNV by comparing the fold change ratio of targeted gene and reference gene. Data from qPCR were analysed using Student t-test. Results: 120 patients diagnosed with dengue were included in this study. 2011 Thesis https://ir.uitm.edu.my/id/eprint/72170/ https://ir.uitm.edu.my/id/eprint/72170/1/72170.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Medicine
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Dengue
spellingShingle	Dengue Mokhtar, Umairah FCGR3B copy number variation and host susceptibility to vascular leakage in dengue hemorrhagic fever / Umairah Mokhtar
description	Dengue is a serious public health threat in many countries around the world. It is estimated that 50- 100 million cases of severe dengue requiring hospitalization occurs annually. Approximately 500,000 of these cases manifested as DHF/ Dengue Shock Syndrome (DSS) with more than 20, 000 deaths worldwide. Dengue deaths occur usually as a complication of severe vascular leakage. The mechanisms that lead to severe vascular leakage are still unknown. Recent studies suggested the potential role of Fe gamma receptors (FCGRs) in the pathogenesis of dengue. In the present study, copy number variation of the FCGR3B genes in a select dengue patient population in Malaysia is estimated. Methodology: Total cell DNA was extracted from dengue patients' whole blood. CNV genotyping was . performed using the real time quantitative PCR (qPCR) with SYBR Green fluorescent dye assay (Bio Rad). In qPCR analysis, the relative quantification was used to analyse relative changes in CNV by comparing the fold change ratio of targeted gene and reference gene. Data from qPCR were analysed using Student t-test. Results: 120 patients diagnosed with dengue were included in this study.
format	Thesis
qualification_level	Bachelor degree
author	Mokhtar, Umairah
author_facet	Mokhtar, Umairah
author_sort	Mokhtar, Umairah
title	FCGR3B copy number variation and host susceptibility to vascular leakage in dengue hemorrhagic fever / Umairah Mokhtar
title_short	FCGR3B copy number variation and host susceptibility to vascular leakage in dengue hemorrhagic fever / Umairah Mokhtar
title_full	FCGR3B copy number variation and host susceptibility to vascular leakage in dengue hemorrhagic fever / Umairah Mokhtar
title_fullStr	FCGR3B copy number variation and host susceptibility to vascular leakage in dengue hemorrhagic fever / Umairah Mokhtar
title_full_unstemmed	FCGR3B copy number variation and host susceptibility to vascular leakage in dengue hemorrhagic fever / Umairah Mokhtar
title_sort	fcgr3b copy number variation and host susceptibility to vascular leakage in dengue hemorrhagic fever / umairah mokhtar
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Medicine
publishDate	2011
url	https://ir.uitm.edu.my/id/eprint/72170/1/72170.pdf
_version_	1794191887197798400

FCGR3B copy number variation and host susceptibility to vascular leakage in dengue hemorrhagic fever / Umairah Mokhtar

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