Embryogenic callus induction of Malaysian rice (Oryza sativa L.) Varieties MR 253 and MR 297 / Erfan Zafran Elias

MR 253 and MR 297 are local Malaysian Oryza sativa L. varieties, were examined for callus induction responses from seed and in vitro plantlets using root part as explants. These varieties were chosen because studies on local Malaysian rice varieties have less findings in terms of plant tissue cultur...

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Main Author:	Elias, Erfan Zafran
Format:	Thesis
Language:	English
Published:	2022
Subjects:	Spermatophyta Phanerogams
Online Access:	https://ir.uitm.edu.my/id/eprint/72922/1/72922.pdf
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id	my-uitm-ir.72922
record_format	uketd_dc
spelling	my-uitm-ir.729222023-02-01T13:53:04Z Embryogenic callus induction of Malaysian rice (Oryza sativa L.) Varieties MR 253 and MR 297 / Erfan Zafran Elias 2022 Elias, Erfan Zafran Spermatophyta. Phanerogams Grain. Cereals MR 253 and MR 297 are local Malaysian Oryza sativa L. varieties, were examined for callus induction responses from seed and in vitro plantlets using root part as explants. These varieties were chosen because studies on local Malaysian rice varieties have less findings in terms of plant tissue culture The explants were treated in MS media with several hormone treatments which added with 2,4-D using 1.0 mg/L, 2.0 mg/L and 3.0 mg/L for both varieties. Callus growth from the seeds and roots became visible after 4 weeks cultured on MS medium supplemented with 2,4-D. Both varieties showed a consistent growth of callus performance. Best treatment was 3.0 mg/L 2,4-D and the average of callus induction frequency were 83.3% and 91.7% for both MR 253 and MR 297 respectively. Then, the best treatment of 3.0 mg/L 2,4-D was proceeded with elicitor addition using casein hydrolysate (1.0, 2.0, 3.0, 4.0 and 5.0 mg/L). Seed was chosen as the best explant to proceed with elicitation treatment. The best treatment with elicitor was 3.0 mg/L 2,4-D + 2.0 mg/L CH and the average of callus induction frequency for MR 253 and MR 297 was 30.0% and 45.0% respectively. Based on the results obtained, comparison of callus growth performance was made between both rice varieties. MR 297 was better than MR 253 because of the callus growth performance consistency. In this experiment, embryogenic callus was obtained in both callus growth by seed and root. First stage of embryogenic callus, globular stage was able to observe by close up microscopic pictures in callus obtained. Thus, it shows the effectiveness and efficiency of using local Malaysian rice varieties in obtaining embryogenic callus. Further experiment may focus more on plant regeneration or callus extraction for secondary metabolites identification. 2022 Thesis https://ir.uitm.edu.my/id/eprint/72922/ https://ir.uitm.edu.my/id/eprint/72922/1/72922.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Plantation and Agrotechnology Awal, Asmah
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Awal, Asmah
topic	Spermatophyta Phanerogams Spermatophyta Phanerogams
spellingShingle	Spermatophyta Phanerogams Spermatophyta Phanerogams Elias, Erfan Zafran Embryogenic callus induction of Malaysian rice (Oryza sativa L.) Varieties MR 253 and MR 297 / Erfan Zafran Elias
description	MR 253 and MR 297 are local Malaysian Oryza sativa L. varieties, were examined for callus induction responses from seed and in vitro plantlets using root part as explants. These varieties were chosen because studies on local Malaysian rice varieties have less findings in terms of plant tissue culture The explants were treated in MS media with several hormone treatments which added with 2,4-D using 1.0 mg/L, 2.0 mg/L and 3.0 mg/L for both varieties. Callus growth from the seeds and roots became visible after 4 weeks cultured on MS medium supplemented with 2,4-D. Both varieties showed a consistent growth of callus performance. Best treatment was 3.0 mg/L 2,4-D and the average of callus induction frequency were 83.3% and 91.7% for both MR 253 and MR 297 respectively. Then, the best treatment of 3.0 mg/L 2,4-D was proceeded with elicitor addition using casein hydrolysate (1.0, 2.0, 3.0, 4.0 and 5.0 mg/L). Seed was chosen as the best explant to proceed with elicitation treatment. The best treatment with elicitor was 3.0 mg/L 2,4-D + 2.0 mg/L CH and the average of callus induction frequency for MR 253 and MR 297 was 30.0% and 45.0% respectively. Based on the results obtained, comparison of callus growth performance was made between both rice varieties. MR 297 was better than MR 253 because of the callus growth performance consistency. In this experiment, embryogenic callus was obtained in both callus growth by seed and root. First stage of embryogenic callus, globular stage was able to observe by close up microscopic pictures in callus obtained. Thus, it shows the effectiveness and efficiency of using local Malaysian rice varieties in obtaining embryogenic callus. Further experiment may focus more on plant regeneration or callus extraction for secondary metabolites identification.
format	Thesis
qualification_level	Master's degree
author	Elias, Erfan Zafran
author_facet	Elias, Erfan Zafran
author_sort	Elias, Erfan Zafran
title	Embryogenic callus induction of Malaysian rice (Oryza sativa L.) Varieties MR 253 and MR 297 / Erfan Zafran Elias
title_short	Embryogenic callus induction of Malaysian rice (Oryza sativa L.) Varieties MR 253 and MR 297 / Erfan Zafran Elias
title_full	Embryogenic callus induction of Malaysian rice (Oryza sativa L.) Varieties MR 253 and MR 297 / Erfan Zafran Elias
title_fullStr	Embryogenic callus induction of Malaysian rice (Oryza sativa L.) Varieties MR 253 and MR 297 / Erfan Zafran Elias
title_full_unstemmed	Embryogenic callus induction of Malaysian rice (Oryza sativa L.) Varieties MR 253 and MR 297 / Erfan Zafran Elias
title_sort	embryogenic callus induction of malaysian rice (oryza sativa l.) varieties mr 253 and mr 297 / erfan zafran elias
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Plantation and Agrotechnology
publishDate	2022
url	https://ir.uitm.edu.my/id/eprint/72922/1/72922.pdf
_version_	1783735960945557504

Embryogenic callus induction of Malaysian rice (Oryza sativa L.) Varieties MR 253 and MR 297 / Erfan Zafran Elias

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