Efficacy of Malaysian propolis as an intracanal medicament against Enterococcus faecalis biofilm: an ex-vivo study / Osamah Hasan Hamood Al-Masoodi

This study aimed to compare the different selected extraction methods of propolis extracts against Enterococcus faecalis (E. faecalis) and to assess and compare the antibacterial activity of calcium hydroxide and an ethanol extract of propolis used as intracanal medicaments on root canals inoculated...

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Main Author:	Hamood Al-Masoodi, Osamah Hasan
Format:	Thesis
Language:	English
Published:	2022
Subjects:	RV Botanic Thomsonian and eclectic medicine SF Animal culture
Online Access:	https://ir.uitm.edu.my/id/eprint/75418/1/75418.pdf
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id	my-uitm-ir.75418
record_format	uketd_dc
spelling	my-uitm-ir.754182023-03-28T11:50:29Z Efficacy of Malaysian propolis as an intracanal medicament against Enterococcus faecalis biofilm: an ex-vivo study / Osamah Hasan Hamood Al-Masoodi 2022 Hamood Al-Masoodi, Osamah Hasan RV Botanic, Thomsonian, and eclectic medicine SF Animal culture This study aimed to compare the different selected extraction methods of propolis extracts against Enterococcus faecalis (E. faecalis) and to assess and compare the antibacterial activity of calcium hydroxide and an ethanol extract of propolis used as intracanal medicaments on root canals inoculated with Enterococcus faecalis. Extraction of Malaysian propolis (MP) was carried out using 70 ethanol by using different selected extraction methods, such as centrifugation-assisted extraction (CAE), vacuum-assisted extraction (VAE), and shaking-assisted extraction (SAE) methods were used. Antimicrobial activity against E. faecalis was assessed using the antibacterial susceptibility test (AST), followed by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Moreover, eighty-four intact human single-rooted teeth were extracted, decoronated and the root canals were chemomechanically prepared. The samples were sterilized before being inoculated with a pure culture of E. faecalis and cultured for 21 days. Prior to medication, colony counts were taken following incubation. Following that, samples were randomly divided into three groups (n = 28). Each group was subsequently subjected to a variety of intracanal medicaments, including Malaysian propolis (group 1), calcium hydroxide [Ca(OH)2] (group 2), and 5% DMSO as a control (group 3). The antibacterial efficacy of each intracanal medicament was determined by calculating the percentage reduction in colony counts (%RCC) on days 1 and 3. The data were analyzed statistically using a one-way analysis of variance and the post hoc Tukey Honestly Significant Difference (HSD) test. 2022 Thesis https://ir.uitm.edu.my/id/eprint/75418/ https://ir.uitm.edu.my/id/eprint/75418/1/75418.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Dentistry Ismail, Ikmal Hisham
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Ismail, Ikmal Hisham
topic	RV Botanic Thomsonian and eclectic medicine SF Animal culture
spellingShingle	RV Botanic Thomsonian and eclectic medicine SF Animal culture Hamood Al-Masoodi, Osamah Hasan Efficacy of Malaysian propolis as an intracanal medicament against Enterococcus faecalis biofilm: an ex-vivo study / Osamah Hasan Hamood Al-Masoodi
description	This study aimed to compare the different selected extraction methods of propolis extracts against Enterococcus faecalis (E. faecalis) and to assess and compare the antibacterial activity of calcium hydroxide and an ethanol extract of propolis used as intracanal medicaments on root canals inoculated with Enterococcus faecalis. Extraction of Malaysian propolis (MP) was carried out using 70 ethanol by using different selected extraction methods, such as centrifugation-assisted extraction (CAE), vacuum-assisted extraction (VAE), and shaking-assisted extraction (SAE) methods were used. Antimicrobial activity against E. faecalis was assessed using the antibacterial susceptibility test (AST), followed by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Moreover, eighty-four intact human single-rooted teeth were extracted, decoronated and the root canals were chemomechanically prepared. The samples were sterilized before being inoculated with a pure culture of E. faecalis and cultured for 21 days. Prior to medication, colony counts were taken following incubation. Following that, samples were randomly divided into three groups (n = 28). Each group was subsequently subjected to a variety of intracanal medicaments, including Malaysian propolis (group 1), calcium hydroxide [Ca(OH)2] (group 2), and 5% DMSO as a control (group 3). The antibacterial efficacy of each intracanal medicament was determined by calculating the percentage reduction in colony counts (%RCC) on days 1 and 3. The data were analyzed statistically using a one-way analysis of variance and the post hoc Tukey Honestly Significant Difference (HSD) test.
format	Thesis
qualification_level	Master's degree
author	Hamood Al-Masoodi, Osamah Hasan
author_facet	Hamood Al-Masoodi, Osamah Hasan
author_sort	Hamood Al-Masoodi, Osamah Hasan
title	Efficacy of Malaysian propolis as an intracanal medicament against Enterococcus faecalis biofilm: an ex-vivo study / Osamah Hasan Hamood Al-Masoodi
title_short	Efficacy of Malaysian propolis as an intracanal medicament against Enterococcus faecalis biofilm: an ex-vivo study / Osamah Hasan Hamood Al-Masoodi
title_full	Efficacy of Malaysian propolis as an intracanal medicament against Enterococcus faecalis biofilm: an ex-vivo study / Osamah Hasan Hamood Al-Masoodi
title_fullStr	Efficacy of Malaysian propolis as an intracanal medicament against Enterococcus faecalis biofilm: an ex-vivo study / Osamah Hasan Hamood Al-Masoodi
title_full_unstemmed	Efficacy of Malaysian propolis as an intracanal medicament against Enterococcus faecalis biofilm: an ex-vivo study / Osamah Hasan Hamood Al-Masoodi
title_sort	efficacy of malaysian propolis as an intracanal medicament against enterococcus faecalis biofilm: an ex-vivo study / osamah hasan hamood al-masoodi
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Dentistry
publishDate	2022
url	https://ir.uitm.edu.my/id/eprint/75418/1/75418.pdf
_version_	1783736053649113088

Efficacy of Malaysian propolis as an intracanal medicament against Enterococcus faecalis biofilm: an ex-vivo study / Osamah Hasan Hamood Al-Masoodi

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