Antimicrobial peptides from the skin secretions of Malaysian frogs from Kuala Pelangai forest reserve / Wan Muhamad Syahmin Wan Ghazali @ Wan Azmi

Amphibians produce substances in their skin that protect against infection by pathogenic microorganisms. Antimicrobial peptides (AMPs) are a diverse class of naturally occurring molecules that exhibit antimicrobial properties, functioning as part of the amphibian’s defence against microbial infectio...

Full description

Saved in:

Bibliographic Details
Main Author:	Wan Ghazali @ Wan Azmi, Wan Muhamad Syahmin
Format:	Thesis
Language:	English
Published:	2022
Subjects:	Chordates Vertebrates QR Microbiology
Online Access:	https://ir.uitm.edu.my/id/eprint/77910/1/77910.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uitm-ir.77910
record_format	uketd_dc
spelling	my-uitm-ir.779102023-05-29T07:21:25Z Antimicrobial peptides from the skin secretions of Malaysian frogs from Kuala Pelangai forest reserve / Wan Muhamad Syahmin Wan Ghazali @ Wan Azmi 2022 Wan Ghazali @ Wan Azmi, Wan Muhamad Syahmin Chordates. Vertebrates QR Microbiology Amphibians produce substances in their skin that protect against infection by pathogenic microorganisms. Antimicrobial peptides (AMPs) are a diverse class of naturally occurring molecules that exhibit antimicrobial properties, functioning as part of the amphibian’s defence against microbial infections. Skin secretions were collected from seven morphologically distinct specimen of Malaysian frogs and screened for antimicrobial activity. The results indicate the several samples of the collected frog skin secretion have antimicrobial effects against Gram-negative and Gram-positive bacteria Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis. Size fractionation chromatography and high performance liquid chromatography (HPLC) was used to purified two samples (samples 3A and 6B) to reveal a single antimicrobial peptide each. Mass spectroscopy fragment profiling of the AMP from sample 3A revealed a probable amino acid sequence of LCQSLPFGGVK. Sequence searches among protein and peptide databases indicate that this peptide does not match any known peptides. The AMP from sample 6B was characterised by Edman end-sequencing to provide an amino acid sequence of ALADDLQCXFIDL. Database search again indicate that this peptide has no sequence similarity to known peptides. Thus, both AMPs could be novel peptides. The frog species that produced these AMPs were preliminarily identified using mitochondrial 16S RNA gene sequence analysis to be Fejervarya multistriata (sample 3A) and Fejevarya limnocharis (sample 6B). Malaysian frogs thus represent a treasure trove of undiscovered AMPs that could be exploited for medicinal purposes. 2022 Thesis https://ir.uitm.edu.my/id/eprint/77910/ https://ir.uitm.edu.my/id/eprint/77910/1/77910.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Abdullah, Mohd Faiz Foong
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Abdullah, Mohd Faiz Foong
topic	Chordates Vertebrates QR Microbiology
spellingShingle	Chordates Vertebrates QR Microbiology Wan Ghazali @ Wan Azmi, Wan Muhamad Syahmin Antimicrobial peptides from the skin secretions of Malaysian frogs from Kuala Pelangai forest reserve / Wan Muhamad Syahmin Wan Ghazali @ Wan Azmi
description	Amphibians produce substances in their skin that protect against infection by pathogenic microorganisms. Antimicrobial peptides (AMPs) are a diverse class of naturally occurring molecules that exhibit antimicrobial properties, functioning as part of the amphibian’s defence against microbial infections. Skin secretions were collected from seven morphologically distinct specimen of Malaysian frogs and screened for antimicrobial activity. The results indicate the several samples of the collected frog skin secretion have antimicrobial effects against Gram-negative and Gram-positive bacteria Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis. Size fractionation chromatography and high performance liquid chromatography (HPLC) was used to purified two samples (samples 3A and 6B) to reveal a single antimicrobial peptide each. Mass spectroscopy fragment profiling of the AMP from sample 3A revealed a probable amino acid sequence of LCQSLPFGGVK. Sequence searches among protein and peptide databases indicate that this peptide does not match any known peptides. The AMP from sample 6B was characterised by Edman end-sequencing to provide an amino acid sequence of ALADDLQCXFIDL. Database search again indicate that this peptide has no sequence similarity to known peptides. Thus, both AMPs could be novel peptides. The frog species that produced these AMPs were preliminarily identified using mitochondrial 16S RNA gene sequence analysis to be Fejervarya multistriata (sample 3A) and Fejevarya limnocharis (sample 6B). Malaysian frogs thus represent a treasure trove of undiscovered AMPs that could be exploited for medicinal purposes.
format	Thesis
qualification_level	Master's degree
author	Wan Ghazali @ Wan Azmi, Wan Muhamad Syahmin
author_facet	Wan Ghazali @ Wan Azmi, Wan Muhamad Syahmin
author_sort	Wan Ghazali @ Wan Azmi, Wan Muhamad Syahmin
title	Antimicrobial peptides from the skin secretions of Malaysian frogs from Kuala Pelangai forest reserve / Wan Muhamad Syahmin Wan Ghazali @ Wan Azmi
title_short	Antimicrobial peptides from the skin secretions of Malaysian frogs from Kuala Pelangai forest reserve / Wan Muhamad Syahmin Wan Ghazali @ Wan Azmi
title_full	Antimicrobial peptides from the skin secretions of Malaysian frogs from Kuala Pelangai forest reserve / Wan Muhamad Syahmin Wan Ghazali @ Wan Azmi
title_fullStr	Antimicrobial peptides from the skin secretions of Malaysian frogs from Kuala Pelangai forest reserve / Wan Muhamad Syahmin Wan Ghazali @ Wan Azmi
title_full_unstemmed	Antimicrobial peptides from the skin secretions of Malaysian frogs from Kuala Pelangai forest reserve / Wan Muhamad Syahmin Wan Ghazali @ Wan Azmi
title_sort	antimicrobial peptides from the skin secretions of malaysian frogs from kuala pelangai forest reserve / wan muhamad syahmin wan ghazali @ wan azmi
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Sciences
publishDate	2022
url	https://ir.uitm.edu.my/id/eprint/77910/1/77910.pdf
_version_	1783736180411465728

Antimicrobial peptides from the skin secretions of Malaysian frogs from Kuala Pelangai forest reserve / Wan Muhamad Syahmin Wan Ghazali @ Wan Azmi

Similar Items