Optimization of nitrofurazone degradation by local Aspergillus tamarii KX610719.1 / Nurul Tasha Zulkifle

Microbial transformation is a biological process during which microorganisms transform organic molecules. Nitrofurazone is widely applied in poultry and aquaculture veterinary drugs. Without appropriate treatment, nitrofurazone residue from agriculture animal waste may have a negative impact on micr...

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Main Author:	Zulkifle, Nurul Tasha
Format:	Thesis
Language:	English
Published:	2022
Subjects:	Bacteria Antibacterial agents
Online Access:	https://ir.uitm.edu.my/id/eprint/78076/1/78076.pdf
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id	my-uitm-ir.78076
record_format	uketd_dc
spelling	my-uitm-ir.780762023-06-01T08:16:34Z Optimization of nitrofurazone degradation by local Aspergillus tamarii KX610719.1 / Nurul Tasha Zulkifle 2022 Zulkifle, Nurul Tasha Bacteria Antibacterial agents Microbial transformation is a biological process during which microorganisms transform organic molecules. Nitrofurazone is widely applied in poultry and aquaculture veterinary drugs. Without appropriate treatment, nitrofurazone residue from agriculture animal waste may have a negative impact on microorganisms. Thus, a study to enhance nitrofurazone degradation using local Aspergillus tamariiKX610719.1 was explored by optimizing the selected parameters. The specific aims of the exploration were: 1) to optimize parameters (pH, temperature and agitation speed)for nitrofurazone degradation rate, 2) to determine the nitrofurazone residue using a High-Performance Liquid Chromatography-diode array detector (HPLC-DAD), 3) to verify the optimum parameters performance in degrading nitrofurazone. Response Surface Methodology (RSM) based on Central Composite Design (CCD) was employed to evaluate and optimize the effect of parameters as independent parameters on the nitro furazone degradation rate as the response function. The interaction effects and optimum parameters were obtained using Design Expert Version 13.0 software (Stat Ease, Inc., Minneapolis, USA). Statistical analysis of variance (ANOVA) with a 95 %confidence level was used to identify the adequacy of the model test and revealed good agreement between the experimental data and the proposed model. The results demonstrate that the optimum conditions for nitrofurazone degradation rate were at thepH value (4.80), temperature (35.84 ºC) and agitation speed (121.33 rpm) with acoefficient of determination, R2 of 0.9612. Based on the verification process, the actual and predicted results was did not significantly differ (p<0.01). After 96 hours of incubation, the percentage of nitrofurazone residue was measured by HPLC-DAD isf ound to be 8.40 %. Aspergillus tamarii KX610719.1 showed a great ability indegrading nitrofurazone under optimum parameters. 2022 Thesis https://ir.uitm.edu.my/id/eprint/78076/ https://ir.uitm.edu.my/id/eprint/78076/1/78076.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Science Zainal Ariffin, Zaidah
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Zainal Ariffin, Zaidah
topic	Bacteria Antibacterial agents
spellingShingle	Bacteria Antibacterial agents Zulkifle, Nurul Tasha Optimization of nitrofurazone degradation by local Aspergillus tamarii KX610719.1 / Nurul Tasha Zulkifle
description	Microbial transformation is a biological process during which microorganisms transform organic molecules. Nitrofurazone is widely applied in poultry and aquaculture veterinary drugs. Without appropriate treatment, nitrofurazone residue from agriculture animal waste may have a negative impact on microorganisms. Thus, a study to enhance nitrofurazone degradation using local Aspergillus tamariiKX610719.1 was explored by optimizing the selected parameters. The specific aims of the exploration were: 1) to optimize parameters (pH, temperature and agitation speed)for nitrofurazone degradation rate, 2) to determine the nitrofurazone residue using a High-Performance Liquid Chromatography-diode array detector (HPLC-DAD), 3) to verify the optimum parameters performance in degrading nitrofurazone. Response Surface Methodology (RSM) based on Central Composite Design (CCD) was employed to evaluate and optimize the effect of parameters as independent parameters on the nitro furazone degradation rate as the response function. The interaction effects and optimum parameters were obtained using Design Expert Version 13.0 software (Stat Ease, Inc., Minneapolis, USA). Statistical analysis of variance (ANOVA) with a 95 %confidence level was used to identify the adequacy of the model test and revealed good agreement between the experimental data and the proposed model. The results demonstrate that the optimum conditions for nitrofurazone degradation rate were at thepH value (4.80), temperature (35.84 ºC) and agitation speed (121.33 rpm) with acoefficient of determination, R2 of 0.9612. Based on the verification process, the actual and predicted results was did not significantly differ (p<0.01). After 96 hours of incubation, the percentage of nitrofurazone residue was measured by HPLC-DAD isf ound to be 8.40 %. Aspergillus tamarii KX610719.1 showed a great ability indegrading nitrofurazone under optimum parameters.
format	Thesis
qualification_level	Master's degree
author	Zulkifle, Nurul Tasha
author_facet	Zulkifle, Nurul Tasha
author_sort	Zulkifle, Nurul Tasha
title	Optimization of nitrofurazone degradation by local Aspergillus tamarii KX610719.1 / Nurul Tasha Zulkifle
title_short	Optimization of nitrofurazone degradation by local Aspergillus tamarii KX610719.1 / Nurul Tasha Zulkifle
title_full	Optimization of nitrofurazone degradation by local Aspergillus tamarii KX610719.1 / Nurul Tasha Zulkifle
title_fullStr	Optimization of nitrofurazone degradation by local Aspergillus tamarii KX610719.1 / Nurul Tasha Zulkifle
title_full_unstemmed	Optimization of nitrofurazone degradation by local Aspergillus tamarii KX610719.1 / Nurul Tasha Zulkifle
title_sort	optimization of nitrofurazone degradation by local aspergillus tamarii kx610719.1 / nurul tasha zulkifle
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Science
publishDate	2022
url	https://ir.uitm.edu.my/id/eprint/78076/1/78076.pdf
_version_	1783736206636351488

Optimization of nitrofurazone degradation by local Aspergillus tamarii KX610719.1 / Nurul Tasha Zulkifle

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