Biological activities of dithiocarbazate schiff base derivatives on normal and diabetic rats / Fathi Farag Mughrabi

The present study was performed to evaluate the acute toxicity, anti-ulcer, wound healing, antioxidant and antibacterial activities of a Schiff base namely benzyl N'-(indol-3-ylmethylidene)-hydrazinecarbodithioate (BIHC) and its metal complexes, BIHCZn and BIHCNi. The three compounds namely BIH...

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Main Author:	Mughrabi, Fathi Farag
Format:	Thesis
Language:	English
Published:	2011
Subjects:	Diseases of the digestive system Gastroenterology
Online Access:	https://ir.uitm.edu.my/id/eprint/7808/2/7808.pdf
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spelling	my-uitm-ir.78082022-11-22T00:33:56Z Biological activities of dithiocarbazate schiff base derivatives on normal and diabetic rats / Fathi Farag Mughrabi 2011 Mughrabi, Fathi Farag Diseases of the digestive system. Gastroenterology Emergency surgery. Wounds and injuries The present study was performed to evaluate the acute toxicity, anti-ulcer, wound healing, antioxidant and antibacterial activities of a Schiff base namely benzyl N'-(indol-3-ylmethylidene)-hydrazinecarbodithioate (BIHC) and its metal complexes, BIHCZn and BIHCNi. The three compounds namely BIHC, BIHCZn and BIHCNi are subsequently collectively referred to as BIHCD. Acute and subchronic toxicity investigation of BIHCD did not show any signs or symptoms of toxicity and all animals remained alive. Blood biochemistry, hematology, and histopathology analysis did not show any significant differences between control and treated groups. BIHCD appears to be safe and no drug-related toxicity was detected even at the highest dose investigated (400mg/kg). Antiulcer activity of BIHCD against ethanol-induced gastric ulcer was studied. Gastric juice was collected for the determination of pH and mucous weight. Grossly, the ulcer control group exhibited severe mucosal injury, whereas pre-treatment with either derivative compounds or Omeprazole resulted in significant protection of gastric mucosal injury. Flattening of gastric mucosal folds was also observed in rats pretreated with BIHCD. Histological studies of the gastric wall of ulcer control group revealed severe damage of gastric mucosa, along with oedema and leucocytes infiltration of the submucosal layer compared to rats pre-treated with either BIHCD or Omeprazole where there were marked gastric protection along with the reduction or absence of oedema and leucocytes infiltration of the submucosal layer. BIHCD and its derivatives promote ulcer protection as ascertained by the comparative decreases in ulcer areas, reduction of oedema and leucocytes infiltration of the submucosal layer. Topical application of BIHC and its metal complexes on the wounds in normal and diabetic rats showed that wound dressed with BIHCD healed significantly earlier than those treated with 10% Tween 20. Also, wound dressed with 100 mg/ml BIHCD showed an accelerated rate of wound healing compared to wounds dressed with Intrasite gel (control), 25 mg/ml, 50 mg/ml and lOOmg/ml BIHCD. Histological analysis of healed wound with BIHCD showed less scar width at wound enclosure and granulation tissue of healed wound contained comparatively less macrophages and large amount of collagen with angiogenesis compared to wounds dressed with 10% Tween 20. Based on the antioxidant activity studies, samples appear to contain low antioxidant properties compared to controls (Trolox, Retin, Ascorbic acid and Gallic acid). For the antibacterial activity, BIHCD appears to possess potential antibacterial activity against Staphylococcus aureus Pseudomonas aeruginosa, Klebsiella Phenumoniae but none against Streptococcus Group A. Determination of minimum inhibitory concentration values showed that BIHCD have less potential antibacterial activity than Gentamicin. 2011 Thesis https://ir.uitm.edu.my/id/eprint/7808/ https://ir.uitm.edu.my/id/eprint/7808/2/7808.pdf text en public phd doctoral Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Hashim, Harita
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Hashim, Harita
topic	Diseases of the digestive system Gastroenterology Diseases of the digestive system Gastroenterology
spellingShingle	Diseases of the digestive system Gastroenterology Diseases of the digestive system Gastroenterology Mughrabi, Fathi Farag Biological activities of dithiocarbazate schiff base derivatives on normal and diabetic rats / Fathi Farag Mughrabi
description	The present study was performed to evaluate the acute toxicity, anti-ulcer, wound healing, antioxidant and antibacterial activities of a Schiff base namely benzyl N'-(indol-3-ylmethylidene)-hydrazinecarbodithioate (BIHC) and its metal complexes, BIHCZn and BIHCNi. The three compounds namely BIHC, BIHCZn and BIHCNi are subsequently collectively referred to as BIHCD. Acute and subchronic toxicity investigation of BIHCD did not show any signs or symptoms of toxicity and all animals remained alive. Blood biochemistry, hematology, and histopathology analysis did not show any significant differences between control and treated groups. BIHCD appears to be safe and no drug-related toxicity was detected even at the highest dose investigated (400mg/kg). Antiulcer activity of BIHCD against ethanol-induced gastric ulcer was studied. Gastric juice was collected for the determination of pH and mucous weight. Grossly, the ulcer control group exhibited severe mucosal injury, whereas pre-treatment with either derivative compounds or Omeprazole resulted in significant protection of gastric mucosal injury. Flattening of gastric mucosal folds was also observed in rats pretreated with BIHCD. Histological studies of the gastric wall of ulcer control group revealed severe damage of gastric mucosa, along with oedema and leucocytes infiltration of the submucosal layer compared to rats pre-treated with either BIHCD or Omeprazole where there were marked gastric protection along with the reduction or absence of oedema and leucocytes infiltration of the submucosal layer. BIHCD and its derivatives promote ulcer protection as ascertained by the comparative decreases in ulcer areas, reduction of oedema and leucocytes infiltration of the submucosal layer. Topical application of BIHC and its metal complexes on the wounds in normal and diabetic rats showed that wound dressed with BIHCD healed significantly earlier than those treated with 10% Tween 20. Also, wound dressed with 100 mg/ml BIHCD showed an accelerated rate of wound healing compared to wounds dressed with Intrasite gel (control), 25 mg/ml, 50 mg/ml and lOOmg/ml BIHCD. Histological analysis of healed wound with BIHCD showed less scar width at wound enclosure and granulation tissue of healed wound contained comparatively less macrophages and large amount of collagen with angiogenesis compared to wounds dressed with 10% Tween 20. Based on the antioxidant activity studies, samples appear to contain low antioxidant properties compared to controls (Trolox, Retin, Ascorbic acid and Gallic acid). For the antibacterial activity, BIHCD appears to possess potential antibacterial activity against Staphylococcus aureus Pseudomonas aeruginosa, Klebsiella Phenumoniae but none against Streptococcus Group A. Determination of minimum inhibitory concentration values showed that BIHCD have less potential antibacterial activity than Gentamicin.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Mughrabi, Fathi Farag
author_facet	Mughrabi, Fathi Farag
author_sort	Mughrabi, Fathi Farag
title	Biological activities of dithiocarbazate schiff base derivatives on normal and diabetic rats / Fathi Farag Mughrabi
title_short	Biological activities of dithiocarbazate schiff base derivatives on normal and diabetic rats / Fathi Farag Mughrabi
title_full	Biological activities of dithiocarbazate schiff base derivatives on normal and diabetic rats / Fathi Farag Mughrabi
title_fullStr	Biological activities of dithiocarbazate schiff base derivatives on normal and diabetic rats / Fathi Farag Mughrabi
title_full_unstemmed	Biological activities of dithiocarbazate schiff base derivatives on normal and diabetic rats / Fathi Farag Mughrabi
title_sort	biological activities of dithiocarbazate schiff base derivatives on normal and diabetic rats / fathi farag mughrabi
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Sciences
publishDate	2011
url	https://ir.uitm.edu.my/id/eprint/7808/2/7808.pdf
_version_	1783733143085252608

Biological activities of dithiocarbazate schiff base derivatives on normal and diabetic rats / Fathi Farag Mughrabi

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