BPA-induced molecular alteration and brain deformities : oligomeric proanthocyanidins intervention study / Fatin Naziha Osman

This study was conducted to assess the effects of Oligomeric Proanthocyanidins (OPC) supplementation on behavioural characteristics and neurogenesis in Bisphenol-A (BPA)-induced rats. Thirty-Six Adult Sprague Dawley male rats were divided into six groups: Normal, BPA (200 mg/kg b.wt. BPA), OPC 10 (1...

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Main Author:	Osman, Fatin Naziha
Format:	Thesis
Language:	English
Published:	2018
Online Access:	https://ir.uitm.edu.my/id/eprint/79190/1/79190.pdf
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id	my-uitm-ir.79190
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spelling	my-uitm-ir.791902023-09-19T07:55:46Z BPA-induced molecular alteration and brain deformities : oligomeric proanthocyanidins intervention study / Fatin Naziha Osman 2018 Osman, Fatin Naziha This study was conducted to assess the effects of Oligomeric Proanthocyanidins (OPC) supplementation on behavioural characteristics and neurogenesis in Bisphenol-A (BPA)-induced rats. Thirty-Six Adult Sprague Dawley male rats were divided into six groups: Normal, BPA (200 mg/kg b.wt. BPA), OPC 10 (10 ug/kg b.wt. OPC), OPC20 (20 ug/kg b.wt. OPC), BPA+OPC10 (200 mg/kg b.wt. BPA + 10 ug/kg b.wt. OPC) and BPA+OPC20 (200 mg/kg b.wt. BPA + 20 ug/kg b.wt. OPC) treated groups. Rats from the respective groups were treated accordingly with the respective dosages, daily, for 21 consecutive days via oral route. Novel Object Discrimination (NOD) test was performed 24 hours after the final administration. The rats were sacrificed after the completion of NOD test. Brains were collected and fixed in 10% formalin, sectioned, stained using cresyl violet staining and examined to assess neurogenesis in hippocampus. Blood was collected and stored at -80°C for chromosome microarray analysis. During NOD test, Normal, OPC10, OPC20, BPA+OPC10 and BPA+OPC20 groups had a significantly longer novel object exploration time as compared to familiar object (p0.05). Results from NOD test suggested that BPA may cause impairment in the rat's ability to remember the previously encountered familiar object while OPC treatment may ameliorate the effect of BPA towards object recognition memory. A significantly thicker dentate gyrus in BPA+OPC10 group (p 2018 Thesis https://ir.uitm.edu.my/id/eprint/79190/ https://ir.uitm.edu.my/id/eprint/79190/1/79190.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Zakaria, Fatin Nadzirah
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Zakaria, Fatin Nadzirah
description	This study was conducted to assess the effects of Oligomeric Proanthocyanidins (OPC) supplementation on behavioural characteristics and neurogenesis in Bisphenol-A (BPA)-induced rats. Thirty-Six Adult Sprague Dawley male rats were divided into six groups: Normal, BPA (200 mg/kg b.wt. BPA), OPC 10 (10 ug/kg b.wt. OPC), OPC20 (20 ug/kg b.wt. OPC), BPA+OPC10 (200 mg/kg b.wt. BPA + 10 ug/kg b.wt. OPC) and BPA+OPC20 (200 mg/kg b.wt. BPA + 20 ug/kg b.wt. OPC) treated groups. Rats from the respective groups were treated accordingly with the respective dosages, daily, for 21 consecutive days via oral route. Novel Object Discrimination (NOD) test was performed 24 hours after the final administration. The rats were sacrificed after the completion of NOD test. Brains were collected and fixed in 10% formalin, sectioned, stained using cresyl violet staining and examined to assess neurogenesis in hippocampus. Blood was collected and stored at -80°C for chromosome microarray analysis. During NOD test, Normal, OPC10, OPC20, BPA+OPC10 and BPA+OPC20 groups had a significantly longer novel object exploration time as compared to familiar object (p0.05). Results from NOD test suggested that BPA may cause impairment in the rat's ability to remember the previously encountered familiar object while OPC treatment may ameliorate the effect of BPA towards object recognition memory. A significantly thicker dentate gyrus in BPA+OPC10 group (p
format	Thesis
qualification_level	Master's degree
author	Osman, Fatin Naziha
spellingShingle	Osman, Fatin Naziha BPA-induced molecular alteration and brain deformities : oligomeric proanthocyanidins intervention study / Fatin Naziha Osman
author_facet	Osman, Fatin Naziha
author_sort	Osman, Fatin Naziha
title	BPA-induced molecular alteration and brain deformities : oligomeric proanthocyanidins intervention study / Fatin Naziha Osman
title_short	BPA-induced molecular alteration and brain deformities : oligomeric proanthocyanidins intervention study / Fatin Naziha Osman
title_full	BPA-induced molecular alteration and brain deformities : oligomeric proanthocyanidins intervention study / Fatin Naziha Osman
title_fullStr	BPA-induced molecular alteration and brain deformities : oligomeric proanthocyanidins intervention study / Fatin Naziha Osman
title_full_unstemmed	BPA-induced molecular alteration and brain deformities : oligomeric proanthocyanidins intervention study / Fatin Naziha Osman
title_sort	bpa-induced molecular alteration and brain deformities : oligomeric proanthocyanidins intervention study / fatin naziha osman
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Sciences
publishDate	2018
url	https://ir.uitm.edu.my/id/eprint/79190/1/79190.pdf
_version_	1783736266161913856

BPA-induced molecular alteration and brain deformities : oligomeric proanthocyanidins intervention study / Fatin Naziha Osman

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