Screening of antagonistic bacteria from healthy pineapple variety MD2 and its potential against bacterial heart rot disease / Naimah Husin

Bacterial heart rot disease caused by pathogenic bacteria Erwinia chrysanthemi is one of the common diseases of pineapple. Current industry practices for controlling the disease through cultural practices and application of chemical synthetic of Benomyl which is not a long lasting control method. Th...

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Main Author:	Husin, Naimah
Format:	Thesis
Language:	English
Published:	2019
Online Access:	https://ir.uitm.edu.my/id/eprint/79342/1/79342.pdf
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id	my-uitm-ir.79342
record_format	uketd_dc
spelling	my-uitm-ir.793422023-09-19T04:56:11Z Screening of antagonistic bacteria from healthy pineapple variety MD2 and its potential against bacterial heart rot disease / Naimah Husin 2019 Husin, Naimah Bacterial heart rot disease caused by pathogenic bacteria Erwinia chrysanthemi is one of the common diseases of pineapple. Current industry practices for controlling the disease through cultural practices and application of chemical synthetic of Benomyl which is not a long lasting control method. This study aims to evaluate the potential of beneficial bacteria isolated from healthy pineapple leaves against the disease pathogen. The disease pathogen was isolated from symptomatic pineapple leaves with the appearance of water soaked and heart rot disease symptoms such as rotten basal tissues. Pathogenicity test was conducted to fulfil the Koch's Postulates. The same procedure for isolation of beneficial bacteria from healthy pineapple plant variety MD2 was carried out. A total of 25 bacterial isolates were successfully isolated from the healthy pineapple leaves and tested against the bacterial pathogen via agar well diffusion method. In vitro assay was utilized to measure the ability of isolated beneficial bacteria in the form of inhibition zone and its growth on media. An isolate identified as Bacillus cereus (BC3) showed the potential of antagonistic activity towards the pathogen. The isolate was further assessed in vivo under rain shelter conditions. The result showed that the isolate was able to suppress the disease symptoms. This study suggested that B. cereus (BC3) could be further explored for its potential as biological control agent for controlling the disease under field condition. 2019 Thesis https://ir.uitm.edu.my/id/eprint/79342/ https://ir.uitm.edu.my/id/eprint/79342/1/79342.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Plantation and Agrotechnology Sapak, Zaiton
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Sapak, Zaiton
description	Bacterial heart rot disease caused by pathogenic bacteria Erwinia chrysanthemi is one of the common diseases of pineapple. Current industry practices for controlling the disease through cultural practices and application of chemical synthetic of Benomyl which is not a long lasting control method. This study aims to evaluate the potential of beneficial bacteria isolated from healthy pineapple leaves against the disease pathogen. The disease pathogen was isolated from symptomatic pineapple leaves with the appearance of water soaked and heart rot disease symptoms such as rotten basal tissues. Pathogenicity test was conducted to fulfil the Koch's Postulates. The same procedure for isolation of beneficial bacteria from healthy pineapple plant variety MD2 was carried out. A total of 25 bacterial isolates were successfully isolated from the healthy pineapple leaves and tested against the bacterial pathogen via agar well diffusion method. In vitro assay was utilized to measure the ability of isolated beneficial bacteria in the form of inhibition zone and its growth on media. An isolate identified as Bacillus cereus (BC3) showed the potential of antagonistic activity towards the pathogen. The isolate was further assessed in vivo under rain shelter conditions. The result showed that the isolate was able to suppress the disease symptoms. This study suggested that B. cereus (BC3) could be further explored for its potential as biological control agent for controlling the disease under field condition.
format	Thesis
qualification_level	Master's degree
author	Husin, Naimah
spellingShingle	Husin, Naimah Screening of antagonistic bacteria from healthy pineapple variety MD2 and its potential against bacterial heart rot disease / Naimah Husin
author_facet	Husin, Naimah
author_sort	Husin, Naimah
title	Screening of antagonistic bacteria from healthy pineapple variety MD2 and its potential against bacterial heart rot disease / Naimah Husin
title_short	Screening of antagonistic bacteria from healthy pineapple variety MD2 and its potential against bacterial heart rot disease / Naimah Husin
title_full	Screening of antagonistic bacteria from healthy pineapple variety MD2 and its potential against bacterial heart rot disease / Naimah Husin
title_fullStr	Screening of antagonistic bacteria from healthy pineapple variety MD2 and its potential against bacterial heart rot disease / Naimah Husin
title_full_unstemmed	Screening of antagonistic bacteria from healthy pineapple variety MD2 and its potential against bacterial heart rot disease / Naimah Husin
title_sort	screening of antagonistic bacteria from healthy pineapple variety md2 and its potential against bacterial heart rot disease / naimah husin
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Plantation and Agrotechnology
publishDate	2019
url	https://ir.uitm.edu.my/id/eprint/79342/1/79342.pdf
_version_	1783736275545620480

Screening of antagonistic bacteria from healthy pineapple variety MD2 and its potential against bacterial heart rot disease / Naimah Husin

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